Thidiazuron stimulates shoot organogenesis and somatic embryogenesis in white ash (Fraxinus americana L.)

1992 ◽  
Vol 31 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Sharon Bates ◽  
John E. Preece ◽  
Nadia E. Navarrete ◽  
J. W. Van Sambeek ◽  
Gerald R. Gaffney
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Organogenesis ◽  
Fraxinus Americana ◽  
White Ash

scholarly journals ADVENTITIOUS SHOOT ORGANOGENESIS IN WHITE ASH (FRAXINUS AMERICANA L.)

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 584e-584
Author(s):  
Sharon Bates ◽  
John E. Preece ◽  
John YOPP ◽  
Robert Trigiano
Keyword(s):  
Silver Nitrate ◽  
Shoot Organogenesis ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Adventitious Buds ◽  
Fraxinus Americana ◽  
White Ash ◽  
Adventitious Shoot Organogenesis

Dissected white ash seeds were placed on a MS basal medium containing 10 μM TDZ and 1 μM 2,4-D. Adventitious buds formed directly and indirectly on cotyledons and hypocotyls that were in contact with the medium. Histological observations after 7 days from initiation indicated early divisional events originated directly in subepidermal layers on adaxial portions of the cotyledons. As these cells divided, the growth ruptured the epidermis. Bud-like structures were seen at 3 weeks. After transfer to a secondary medium containing 3 μM TDZ, 1 μM BA, and 1 μM IBA, some of adventitious buds elongated. Efforts (gibberellin, etiolation, ABA, and silver nitrate treatments) to increase the number of elongated buds have been unsuccessful. Excised adventitious shoots were rooted under mist and established in the greenhouse.

FRAXINUS AMERICANA, WHITE ASH

2014 ◽  
pp. 901-905
Author(s):  
Henry John Elwes ◽  
Augustine Henry
Keyword(s):  
Fraxinus Americana ◽  
White Ash

Plant regeneration from protoplasts of Vicia narbonensis via somatic embryogenesis and shoot organogenesis

1996 ◽  
Vol 16 (1-2) ◽  
pp. 22-25
Author(s):  
Mechthild Tegeder ◽  
Hubertus Kohn ◽  
Matthias Nibbe ◽  
Otto Schieder ◽  
Thomas Pickardt
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Shoot Organogenesis ◽  
Vicia Narbonensis
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