Genotypic and media effects on plant regeneration from cotyledon explant cultures of someBrassica species

1988 ◽  
Vol 14 (3) ◽  
pp. 197-206 ◽  
Author(s):  
R. K. Jain ◽  
J. B. Chowdhury ◽  
D. R. Sharma ◽  
W. Friedt
1990 ◽  
Vol 40 (3) ◽  
pp. 321-327 ◽  
Author(s):  
Qing-chang LIU ◽  
Teiji KOKUBU ◽  
Muneharu SATO

1999 ◽  
Vol 45 (3) ◽  
pp. 639-646 ◽  
Author(s):  
Reda E.A. Moghaieb ◽  
Hirofumi Saneoka ◽  
Kounosuke Fujita

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 618c-618 ◽  
Author(s):  
Hyo-Geur. Park ◽  
Kyung-Young Choi ◽  
Do-Hyun Lee

Hot pepper is the most important vegetable in Korea in terms of both acreage and product value. Morphogenic response in tissue culture of hot pepper varied greatly with explant source and growth regulators. Somatic enbryogenesis, which we believe had not been reported yet, was only induced from 5-month-old callus derived from anther culture. Embryogenic callus was solely observed in the MS medium supplemented with 2 mg/l of NAA and 2 mg/l of BA. Better somatic embryogenesis and plant regeneration was observed on hormone free medium. Adventitious organogenesis was well induced from both hypocotyl and cotyledon. Polarity of explant for shoot regeneration was observed. Success rate of regeneration has been continuously improved mainly due to combinations of growth regulators. The best combination so far was 2 mg/l of zeatin and 1 mg/l of IAA, resulting in 92% of regeneration from cotyledon explant.


Sign in / Sign up

Export Citation Format

Share Document