Large scale fermentation and alkaloid production of cell suspension cultures of Catharanthus roseus

1987 ◽  
Vol 8 (2) ◽  
pp. 153-161 ◽  
Author(s):  
Otmar Schiel ◽  
Jochen Berlin
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Large Scale ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Alkaloid Production

Monoterpenoid Oxindole Alkaloid Production by Uncaria tomentosa (Willd) D.C. Cell Suspension Cultures in a Stirred Tank Bioreactor

2008 ◽  
Vol 21 (3) ◽  
pp. 786-792 ◽  
Author(s):  
Gabriela Trejo-Tapia ◽  
Carlos M. Cerda-García-Rojas ◽  
Mario Rodríguez-Monroy ◽  
Ana C. Ramos-Valdivia
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Stirred Tank ◽  
Alkaloid Production ◽  
Stirred Tank Bioreactor ◽  
Uncaria Tomentosa

scholarly journals Biotransformation of Cyclodextrine-Complexed Semisynthetic Betulin Derivatives by Plant Cells

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 743-748
Author(s):  
Suvi Häkkinen ◽  
Heli Nygren ◽  
Natalia Maiorova ◽  
Raisa Haavikko ◽  
Sami Alakurtti ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Plant Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Water Soluble ◽  
Betulonic Acid ◽  
Poorly Water Soluble ◽  
Betulin Derivatives

AbstractIn this study, three semisynthetic betulonic acid-based compounds, 20(29)-dihydrolup-2-en[2,3-d]isoxazol-28-oic acid, 1-betulonoylpyrrolidine, and lupa-2,20(29)-dieno[2,3-b]pyrazin-28-oic acid, were studied in biotransformation experiments using Nicotiana tabacum and Catharanthus roseus cell suspension cultures. Biotransformation was performed using cyclodextrin to aid dissolving poorly water-soluble substrates. Several new derivatives were found, consisting of oxidized and glycosylated (pentose- and hexose-conjugated) products.

Regioselective hydroxylation of warfarin by cell suspension cultures of Catharanthus roseus

1993 ◽  
Vol 33 (3) ◽  
pp. 599-600 ◽  
Author(s):  
Hiroki Hamada ◽  
Yoshihiro Fuchikami ◽  
Robert L. Jansing ◽  
Laurence S. Kaminsky
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regioselective Hydroxylation

Influence of Medium Composition on the Formation of Secondary Compounds in Cell Suspension Cultures of Catharanthus roseus (L.) G. Don

1980 ◽  
Vol 35 (7-8) ◽  
pp. 551-556 ◽  
Author(s):  
K.-H. Knobloch ◽  
J. Berlin
Keyword(s):  
Phenolic Compounds ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Culture Media ◽  
Sucrose Solution ◽  
Cell Suspension Cultures ◽  
Secondary Compounds ◽  
Suspension Cultures ◽  
Medium Composition ◽  
High Concentrations

Cell suspension cultures of Catharanthus roseus have been subjected to various media condi­tions in order to stimulate the formation of indole alkaloids. High ajmalicine contents (up to 0.5 mg/g cell fresh weight) were achieved by transferring 2-week-old cell suspensions to a 10-fold volume of a 8% sucrose solution. The alkaloid accumulation started two days after the transfer and reached a plateau after ten days. Furthermore an enhanced level of phenolic compounds was found, whereas growth of the culture was low. The accumulation of both, alkaloids and poly­phenols was stimulated by high concentrations of sucrose and low concentrations of nitrogen con­taining salts and phosphate. When these minerals were added to the sucrose solution in con­centrations commonly used for cell culture media, the accumulation of alkaloids and phenolic compounds was largely suppressed.

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