Chromosomal location of genes controlling 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase isozymes in cultivated rye

Euphytica ◽  
1983 ◽  
Vol 32 (3) ◽  
pp. 783-790 ◽  
Author(s):  
J. Salinas ◽  
C. Benito
Keyword(s):  
Glutamate Dehydrogenase ◽  
Chromosomal Location ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose 6 Phosphate Dehydrogenase

Constancy of enzyme electrofocusing patterns in a stand of the lichen Umbilicaria mammulata

1986 ◽  
Vol 64 (9) ◽  
pp. 1928-1934 ◽  
Author(s):  
C. Hageman ◽  
D. Fahselt
Keyword(s):  
Alkaline Phosphatase ◽  
Superoxide Dismutase ◽  
Glutamate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Mannitol Dehydrogenase ◽  
Specific Staining ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Sampling Times ◽  
Umbilicaria Mammulata

Thalli of the lichen Umbilicaria mammulata (Ach.) Tuck, were collected from one site, 24 at each of five different sampling times, over a period of 1 year. Protein extracts from each individual thallus were subjected to isoelectric focusing, followed by specific staining for eight enzyme systems (mannitol dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, superoxide dismutase, esterase, and alkaline phosphatase). A total of 55 bands were resolved, 25 of which were constant in all thalli through all sampling dates. Principal components analysis of the electromorph data showed the existence of groups corresponding to sets of thalli collected at the same times; esterase and alkaline phosphatase variation were primarily responsible for these groupings. Banding patterns of 6-phosphogluconate dehydrogenase and superoxide dismutase were constant regardless of date of collection. Most bands of glucose-6-phosphate dehydrogenase and glutamate dehydrogenase were consistent through all sampling times, but each system exhibited one band that differed in frequency from one collection date to another. Electromorphs of mannitol dehydrogenase and isocitrate dehydrogenase exhibited only minor variability. Dehydrogenase enzymes, since they tended to vary least from one sampling time to the next, can thus be used more readily for taxonomic purposes. If esterases and alkaline phosphatases are to be applied as taxonomic criteria, samples for comparison should be collected simultaneously.

Inheritance of needle tissue isozymes in Douglas-fir

1984 ◽  
Vol 26 (4) ◽  
pp. 459-468 ◽  
Author(s):  
D. B. Neale ◽  
J. C. Weber ◽  
W. T. Adams
Keyword(s):  
Glutamate Dehydrogenase ◽  
Pseudotsuga Menziesii ◽  
Isocitrate Dehydrogenase ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Glutamate Oxaloacetate Transaminase ◽  
Electrophoretic Variants ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase

Methods for resolving electrophoretic variants from extracts of needle tissue of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco) are described, and the inheritance of 12 of at least 15 loci that control allozymes from 11 enzyme systems are established. Evidence for the inheritance of allozyme variants was obtained in three ways: (i) comparison in seed orchard clones of allozyme genotypes determined from both megagametophyte and needle tissue; (ii) analysis of segregating full-sib progenies of seed orchard clones; and (iii) comparison of needle allozyme pattern phenotypes to previously reported embryo phenotypes. Ten of the 12 loci (coding phosphoglucomutase, PGM(1) and PGM(2); glycerate dehydrogenase, GLYDH; phosphoglucose isomerase, PG1(2); glutamate dehydrogenase, GDH; glucose-6-phosphate dehydrogenase, G-6PD; 6-phosphogluconate dehydrogenase, 6-PGD(1); isocitrate dehydrogenase, IDH; diaphorase, DIA(2); malate dehydrogenase, MDH(1)) produce clear bands in seed tissue; however, glutamate oxaloacetate transaminase GOT(3) (N) was not found in seeds and shikimic dehydrogenase (SDH) could only be clearly resolved in needles (N). Several enzymes active in seed tissue could not be detected in needle tissues.Key words: Douglas-fir, needle tissue isozymes, inheritance.

CHANGES IN ENZYMES OF CARBOHYDRATE METABOLISM IN RAT UTERUS DURING EARLY PREGNANCY

1971 ◽  
Vol 68 (4) ◽  
pp. 805-816 ◽  
Author(s):  
M. A. H. Surani ◽  
P. J. Heald
Keyword(s):  
Energy Requirement ◽  
Lipid Synthesis ◽  
Dry Weight ◽  
Glycolytic Enzymes ◽  
Rat Uterus ◽  
Malic Dehydrogenase ◽  
Phosphogluconate Dehydrogenase ◽  
Tissue Changes ◽  
Implantation Sites ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT The enzymes phosphofructokinase (PFK), pyruvate kinase (PK), isocitric dehydrogenase (ICDH), malic dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G-6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) have been measured in rat uterus during the first 9 days of pregnancy. It was found that after implantation on day 6, the activities of PFK and PK (the key glycolytic enzymes) increased in terms of dry weight — and in terms of protein in the implantation sites, but decreased in non-implanted tissue. The pentose shunt enzymes changed similarly to those of the glycolytic enzymes. ICDH activity increased in the non-implanted tissue and decreased in the implanted tissue. Changes in malic dehydrogenase were extremely variable and did not show a consistent pattern. Administration of Actinomycin D on day 6 of pregnancy abolished the increase in PK and PFK in the implantation sites and indeed led to a major decrease in activity. This implies that the increased PK and PFK in the implantation sites, arise from a DNA dependent RNA directed synthesis of new enzyme protein. The results are discussed in relation to the energy requirement of the decidualising tissue and the need for increased pentose for RNA synthesis. It is suggested that the extra NADPH resulting from the pentose shunt is involved in increased lipid synthesis.

EINFLUSS VON CYCLUS UND EXOGENER HORMONZUFUHR AUF STOFFWECHSELVORGÄNGE DES RATTENUTERUS

1967 ◽  
Vol 56 (2) ◽  
pp. 231-243 ◽  
Author(s):  
H. Schmidt ◽  
I. Noack ◽  
H. Walther ◽  
K. D. Voigt
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Leucine Aminopeptidase ◽  
Intraperitoneal Administration ◽  
Exogenous Hormone ◽  
Rat Uterus ◽  
Normal Cycle ◽  
Phosphogluconate Dehydrogenase ◽  
Dna And Rna ◽  
Hormone Administration ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT The first significant increase of weight, RNA and protein was observed in the uterus of spayed rats twelve hours after the intraperitoneal administration of a single dose of 1 μg oestradiol. There was no significant increase of DNA. At the same time the activities of glucose-6-phosphate dehydrogenase, fructose-1,6-diphosphate aldolase, isocitrate dehydrogenase and leucine aminopeptidase had increased significantly. Twentyfour hours after the injection the augmented values began to decline. Three injections of 1 μg oestradiol, given at 24 hour intervals obtained similar changes, the only difference being that these changes were more marked and that a DNA increase was also observed. The augmentation of protein, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and fructose-1,6-diphosphate aldolase content of cells induced by repeated oestradiol injections was inhibited partly by 1 mg progesterone when administered together with the last dose of oestradiol. During the normal oestrus cycle of the rat uterus an increase of uterine weight, DNA and RNA content and also of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and 1,6-diphosphate aldolase activities was observed, whereas isocitrate dehydrogenase, malate dehydrogenase and leucine aminopeptidase did not change significantly. It would appear that the changes after exogenous hormone administration reflect those of the normal cycle as regards both their extent and timing. The importance of these findings in connection with hormone-induced pathways of uterine metabolism is discussed.

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