Comparison of different constitutive and inducible promoters for the overexpression of transgenes in Arabidopsis thaliana

1995 ◽  
Vol 29 (4) ◽  
pp. 637-646 ◽  
Author(s):  
S�nke Holtorf ◽  
Klaus Apel ◽  
Holger Bohlmann
2008 ◽  
Vol 95 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Suchandra Deb Roy ◽  
Mukesh Saxena ◽  
Prasanna S. Bhomkar ◽  
Mikhail Pooggin ◽  
Thomas Hohn ◽  
...  

2005 ◽  
Vol 46 (4) ◽  
pp. 547-556 ◽  
Author(s):  
Takeshi Masaki ◽  
Naoko Mitsui ◽  
Hironaka Tsukagoshi ◽  
Terumi Nishii ◽  
Atsushi Morikami ◽  
...  

2018 ◽  
Vol 97 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Neeraj Kumar Dubey ◽  
Devesh Kumar Mishra ◽  
Asif Idris ◽  
Deepti Nigam ◽  
Pradhyumna Kumar Singh ◽  
...  

2021 ◽  
Vol 22 (4) ◽  
pp. 1971
Author(s):  
Pramod Rathor ◽  
Tudor Borza ◽  
Sophia Stone ◽  
Thierry Tonon ◽  
Svetlana Yurgel ◽  
...  

Brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae that evolved complex multicellularity. Ectocarpus sp. is a dominant seaweed in temperate regions, abundant mostly in the intertidal zones, an environment with high levels of abiotic stresses. Previous transcriptomic analysis of Ectocarpus sp. revealed several genes consistently induced by various abiotic stresses; one of these genes is Esi0017_0056, which encodes a protein with unknown function. Bioinformatics analyses indicated that the protein encoded by Esi0017_0056 is soluble and monomeric. The protein was successfully expressed in Escherichia coli,Arabidopsis thaliana and Nicotiana benthamiana. In A. thaliana the gene was expressed under constitutive and stress inducible promoters which led to improved tolerance to high salinity and temperature stresses. The expression of several key abiotic stress-related genes was studied in transgenic and wild type A. thaliana by qPCR. Expression analysis revealed that genes involved in ABA-induced abiotic stress tolerance, K+ homeostasis, and chaperon activities were significantly up-regulated in the transgenic line. This study is the first report in which an unknown function Ectocarpus sp. gene, highly responsive to abiotic stresses, was successfully expressed in A. thaliana, leading to improved tolerance to salt and temperature stress.


1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  

1995 ◽  
Vol 95 (2) ◽  
pp. 260-266 ◽  
Author(s):  
Maarten Koornneef ◽  
Corrie Hanhart ◽  
Patty van Loenen-Martinet ◽  
Hetty Blankestijn de Vries

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document