Ultrastructural and protein changes in cell suspension cultures of peach associated with low temperature-induced cold acclimation and abscisic acid treatment

1995 ◽  
Vol 40 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Rajeev Arora ◽  
Michael E. Wisniewski
Keyword(s):  
Abscisic Acid ◽  
Low Temperature ◽  
Cell Suspension ◽  
Cold Acclimation ◽  
Acid Treatment ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Abscisic acid and low temperature induced polypeptide changes in alfalfa (Medicago sativa) cell suspension cultures

1986 ◽  
Vol 64 (11) ◽  
pp. 2758-2763 ◽  
Author(s):  
Albert J. Robertson ◽  
Lawrence V. Gusta
Keyword(s):  
Abscisic Acid ◽  
Low Temperature ◽  
Medicago Sativa ◽  
Cell Suspension ◽  
Sodium Dodecyl ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Total Cell ◽  
Cell Protein ◽  
Major Protein

Changes in extracellular, cellular, and subcellular proteins during abscisic acid and low temperature induced cold hardening of alfalfa (Medicago sativa L. cv. Wisconsin 22C) cell suspension cultures were investigated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Extracellular proteins from 4- to 6-day-old ABA and low temperature grown alfalfa cells showed decreased electrophoretic mobilities, lacked a 190-kDa glycoprotein, and had reduced amounts of four other polypeptides. In total cell protein analyses, a 42-kDa protein was enriched in both ABA and low temperature treated alfalfa cells. Several proteins increased or induced by exogenous ABA treatment were identified in the extracellular (12.5 and 13 to 15 kDa), total cell and cell wall (24 kDa), and soluble (20, 37, and 41 kDa) fractions. However, no major protein changes were resolved by one-dimensional electrophoretic analyses of crude membrane proteins.

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Fuyuan Jing ◽  
Ling Zhang ◽  
Meiya Li ◽  
Yueli Tang ◽  
Yuliang Wang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Cell Suspension ◽  
Biosynthetic Pathway ◽  
Gene Expression Analysis ◽  
Artemisia Annua ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Expression Of Genes ◽  
Artemisinin Content

AbstractArtemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L., is the most effective antimalarial drug. In an effort to increase the artemisinin production, abscisic acid (ABA) with different concentrations (1, 10 and 100 µM) was tested by treating A. annua plants. As a result, the artemisinin content in ABA-treated plants was significantly increased. Especially, artemisinin content in plants treated by 10 µM ABA was 65% higher than that in the control plants, up to an average of 1.84% dry weight. Gene expression analysis showed that in both the ABA-treated plants and cell suspension cultures, HMGR, FPS, CYP71AV1 and CPR, the important genes in the artemisinin biosynthetic pathway, were significantly induced. While only a slight increase of ADS expression was observed in ABA-treated plants, no expression of ADS was detected in cell suspension cultures. This study suggests that there is probably a crosstalk between the ABA signaling pathway and artemisinin biosynthetic pathway and that CYP71AV1, which was induced most significantly, may play a key regulatory role in the artemisinin biosynthetic pathway.

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