A description of the Rubisco large subunit gene and its transcript in Olisthodiscus luteus

1992 ◽  
Vol 18 (3) ◽  
pp. 595-599 ◽  
Author(s):  
Linda K. Hardison ◽  
Barbara A. Boczar ◽  
Ann E. Reynolds ◽  
Rose Ann Cattolico
2021 ◽  
Author(s):  
Myat T. Lin ◽  
Douglas J. Orr ◽  
Dawn Worrall ◽  
Martin A. J. Parry ◽  
Elizabete Carmo‐Silva ◽  
...  

Plant Science ◽  
2005 ◽  
Vol 169 (5) ◽  
pp. 882-893 ◽  
Author(s):  
Jinpeng Xing ◽  
Xiangyang Li ◽  
Yuying Luo ◽  
Thomas J. Gianfagna ◽  
Harry W. Janes

1985 ◽  
Vol 5 (10) ◽  
pp. 2733-2745 ◽  
Author(s):  
L Hanley-Bowdoin ◽  
E M Orozco ◽  
N H Chua

The large subunit gene (rbcL) of ribulose 1,5-bisphosphate carboxylase was transcribed in vitro by using maize and pea chloroplast extracts and a cloned plastid DNA template containing 172 base pairs (bp) of the maize rbcL protein-coding region and 791 bp of upstream sequences. Three major in vitro RNA species were synthesized which correspond to in vivo maize rbcL RNAs with 5' termini positioned 300, 100 to 105, and 63 nucleotides upstream of the protein-coding region. A deletion of 109 bp, including the "-300" 5' end (the 5' end at position -300), depressed all rbcL transcription in vitro. A plasmid DNA containing this 109-bp fragment was sufficient to direct correct transcription initiation in vitro. A cloned template, containing 191 bp of plastid DNA which includes the -105 and -63 rbcL termini, did not support transcription in vitro. Exogenously added -300 RNA could be converted to the -63 transcript by maize chloroplast extract. These results established that the -300 RNA is the primary maize rbcL transcript, the -63 RNA is a processed form of the -300 transcript, and synthesis of the -105 RNA is dependent on the -300 region. The promoter for the maize rbcL gene is located within the 109 bp flanking the -300 site. Mutagenesis of the 109-bp chloroplast sequence 11 bp upstream of the -300 transcription initiation site reduced rbcL promoter activity in vitro.


2018 ◽  
Vol 365 (14) ◽  
Author(s):  
Xiang-Qun Chi ◽  
Long Wang ◽  
Ruoyu Guo ◽  
Dexi Zhao ◽  
Jia Li ◽  
...  

Cell ◽  
1983 ◽  
Vol 34 (3) ◽  
pp. 1007-1014 ◽  
Author(s):  
David M. Lonsdale ◽  
Tony P. Hodge ◽  
Christopher J. Howe ◽  
David B. Stem

2020 ◽  
Vol 8 (2) ◽  
pp. 14-21
Author(s):  
Trinh Van Hanh ◽  
Vu Tien Luyen ◽  
Pham Huu Hung ◽  
Truong Binh Nguyen ◽  
Tran Duc Vinh ◽  
...  

Fungal species of Cantharellales order are found worldwide including Europe, Africa, Asia, and North America. This clade includes two smaller, separate, but closely related genera Cantharellus and Craterellus. Cantharellus was firstly identified in the middle of the 17th century. Besides traditional classification based on morphology, molecular phylogeny of the nuclear ribosomal large subunit gene (nrLSU) can be an effective tool to provide more information during species identification for this group. In this current report, a dataset of the nrLSU gene including 60 sequences of Cantharellus species and 1 sequence belonging to Craterellus (outgroup), was obtained from Genbank. Phylogenetic analyses by MEGA 6.0 showed a monophyletic relationship between the six well-established clades, namely Cantharellus (Clade 1), Rubrinus (Clade 2), Cinnabarinus (Clade 3), Parvocantharellus (Clade 4), Pseudocantharellus (Clade 5) and subgenus Afrocantharellus (Clade 6) similar to that of morphological classification. Therefore, this dataset is efficient to support the identification of the fungal samples, collected at the Langbian Mountain, Lam Dong.


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