Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene

1993 ◽  
Vol 22 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Udo Johanningmeier ◽  
Silvia Heiss
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Psba Gene

Nonrandom distribution of chloroplast recombination events in Chlamydomonas reinhardtii: evidence for a hotspot and an adjacent cold region.

Genetics ◽  
1992 ◽  
Vol 132 (2) ◽  
pp. 413-429
Author(s):  
S M Newman ◽  
E H Harris ◽  
A M Johnson ◽  
J E Boynton ◽  
N W Gillham
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Point Mutations ◽  
Chloroplast Transformation ◽  
Psba Gene ◽  
Reading Frame ◽  
Recombination Hotspots ◽  
Chloroplast Genomes ◽  
Intergenic Regions ◽  
Sexual Crosses ◽  
Rna Genes

Abstract Intermolecular recombination of Chlamydomonas chloroplast genes has been analyzed in sexual crosses and following biolistic transformation. The pattern and position of specific exchange events within 15 kb of the 22-kb inverted repeat have been mapped with respect to known restriction fragment length polymorphism markers that distinguish the chloroplast genomes of the interfertile species Chlamydomonas reinhardtii and Chlamydomonas smithii. Recombinant progeny were selected from two- and three-factor crosses involving point mutations conferring herbicide (dr) and antibiotic resistance (er and spr) in the psbA, 23S and 16S ribosomal RNA genes, respectively. Exchange events were not randomly distributed over the 15-kb region, but were found to occur preferentially in a 0.7-kb sequence spanning the 3' end of the psbA gene and were much less common in an adjacent region of ca. 2.0 kb. These findings are corroborated by data showing that the dr mutation is unlinked genetically (3% recombination/kb) to the er and spr rRNA mutations, which are themselves linked and show ca. 1% recombination/kb. This discrepancy is significant since the dr-er and er-spr intervals are about the same length (ca. 7 kb). During chloroplast transformation, the 0.7-kb recombination hotspot also functions as a preferential site for exchange events leading to the integration of donor psbA gene sequences. The 0.7-kb hotspot region contains four classes of 18-37-bp direct repeats also found in other intergenic regions, but no open reading frame. Using deletion constructs in a chloroplast transformation assay, the hotspot was localized to a 500-bp region that lacks most of these repeats, which suggests that the repeats themselves are not responsible for the increased recombination frequency. Within this region, a 400-bp sequence is highly conserved between the chloroplast genomes of C. reinhardtii and C. smithii and includes several structural motifs characteristic of recombination hotspots in other systems.

Site-Directed Mutagenesis of the psbA Gene in the Green Alga, Chlamydomonas reinhardtii

1995 ◽  
pp. 703-706
Author(s):  
Yuichiro Takahashi ◽  
Keiichiro Utsumi ◽  
Aya Hatano ◽  
Yumiko Yamamoto ◽  
Kimiyuki Satoh
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Psba Gene

A nuclear mutation affects the synthesis of the chloroplast psbA gene production Chlamydomonas reinhardtii

1992 ◽  
Vol 22 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Jacqueline Girard-Bascou ◽  
Yves Pierre ◽  
Dominique Drapier
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Psba Gene ◽  
Nuclear Mutation
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