Inheritance of resistance to cucumber green mottle mosaic virus in muskmelon (Cucumis melo L.)

Euphytica ◽  
1990 ◽  
Vol 47 (2) ◽  
pp. 93-97 ◽  
Author(s):  
L. Rajamony ◽  
T. A. More ◽  
V. S. Seshadri
Keyword(s):  
Mosaic Virus ◽  
Cucumis Melo ◽  
Inheritance Of Resistance ◽  
Cucumis Melo L

scholarly journals Inheritance of Resistance to Cucumber Green Mottle Mosaic Virus in Cucumis melo L. ‘Chang Bougi’

2007 ◽  
Vol 76 (4) ◽  
pp. 316-318 ◽  
Author(s):  
Mitsuhiro Sugiyama ◽  
Takayoshi Ohara ◽  
Yoshiteru Sakata
Keyword(s):  
Mosaic Virus ◽  
Cucumis Melo ◽  
Inheritance Of Resistance ◽  
Cucumis Melo L

scholarly journals Squash Mosaic Virus Isolated from Melon (Cucumis melo L.) in Hokkaido

1980 ◽  
Vol 46 (3) ◽  
pp. 349-356 ◽  
Author(s):  
Kouji YOSHIDA ◽  
Tadanori GOTO ◽  
Masayasu NEMOTO ◽  
Tsuneo TSUCHIZAKI
Keyword(s):  
Mosaic Virus ◽  
Cucumis Melo ◽  
Cucumis Melo L

scholarly journals First report of Cucurbit chlorotic yellows virus infecting Cucumis melo (muskmelon and oriental melon) in Korea

Plant Disease ◽  
2021 ◽  
Author(s):  
In Sook Cho ◽  
Tae-Bok Kim ◽  
Ju-Yeon Yoon ◽  
Bong Nam Chung ◽  
John Hammond ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Cucumis Melo ◽  
Korean Isolate ◽  
Rt Pcr ◽  
Genome Database ◽  
Total Rna ◽  
First Report ◽  
Oriental Melon ◽  
Cucumis Melo L ◽  
Cucurbit Chlorotic Yellows Virus

In December 2018, virus-like symptoms (yellowing, vein clearing) were observed on 2% of muskmelon (Cucumis melo L.) plants in plastic houses on a farm in Gyeongsang province, Korea Total RNA from two symptomatic and two asymptomatic plants was extracted using RNeasy Plant Mini Kit (Qiagen, Germany) for high throughput sequencing (HTS). After pre-processing and Ribo-Zero rRNA removal, a cDNA library was prepared (Illumina TruSeq Stranded Total RNA kit) and sequenced (Illumina NovaSeq 6000 system: Macrogen Inc. Korea). De novo assembly of 88,222,684 HTS reads with Trinity software (r20140717) yielded 146,269 contigs of 201-28,442 bp, which were screened against the NCBI viral genome database by BLASTn. Contigs from cucumber mosaic virus (CMV), melon necrotic spot virus (MNSV), tobacco mosaic virus (TMV) and watermelon mosaic virus (WMV) were identified, all previously reported in Korea. Two contigs (8,539 and 8,040 bp) with 99.9% sequence identity to distinct cucurbit chlorotic yellows virus (CCYV) isolates (JN641883, RNA1, Taiwan; MH819191, RNA2, China) were also identified. The ten sequences most closely related to each RNA of the Korean isolate (≥99% coverage, ≥99.6% nt identity) were from Japan, China, Taiwan, or Israel. CCYV presence was confirmed by reverse transcription-PCR (RT-PCR) using newly designed specific primers, RdRp-F/RdRp-R (5’-ACCGAACACTTGGCTATCCAA-3’/5’-CTTAATGCCGCGTATGAACTCA-3’) span style="font-family:'Times New Roman'; letter-spacing:-0.5pt">and HSP-F/HSP-R (5’-TGAACGACACTGAGTTCATTCCTA-3’/5’-CGCCAAGATCGTACATGAGGAA-3’), against RNA dependent RNA polymerase (RdRp; RNA1) and the heat shock protein 70 homolog (HSP70h; RNA2). Symptomatic samples yielded products of expected sizes (RdRp,450 bp; HSP70h, 510 bp) while asymptomatic samples did not. The amplicons were cloned, and two clones of each were sequenced (BIONEER, Korea; GenBank acc. nos. LC592226 and LC592227) showing 100% and 99.2% nt identity with RdRp and HSP70h genes of Chinese CCYV isolate SD (MH819190 and MH819191, respectively) and other Asian isolates. Primers specific for CMV, WMV, beet pseudo-yellows virus (BPYV) (Okuda et al., 2007), TMV (Kim et al., 2018), MNSV (F/R, 5ʹ-ATCTCGCATTTGGCATTACTC-3ʹ/5ʹ-ATTTGTAGAGATGCCAACGTA-3ʹ), cucurbit yellow stunting disorder virus (CYSDV; Zeng et al., 2011) and cucurbit aphid-borne yellows virus (CABYV; F/R, 5ʹ-CGGTCTATTGTCTGCAGTACCA-3ʹ/5ʹ- GTAGAGGATCTTGAATTGGTCCTCA-3ʹ) were also used. None of these viruses were detected in the symptomatic samples, but both asymptomatic plants were positive for CMV and WMV, and one also for MNSV. In June and September 2020, muskmelon and oriental melon (Cucumis melo L. var. makuwa) plants with yellowing disease (incidence 80-90%) and whiteflies were observed in all investigated plastic houses of one muskmelon and one oriental melon farm in Gyeonggi and Jeolla provinces. Symptomatic samples (14 muskmelon; 6 oriental melon) were collected and RT-PCR tested as above; 19/20 samples were positive for CCYV, but none for the other viruses. The oriental melon sequence (LC592895, LC592230) showed 99.7% and 100% nt identity with the RdRp and HSP70h genes of Chinese isolate SD, respectively. CCYV was first reported in Japan (Okuda et al., 2010), Taiwan, and China (Huang et al., 2010; Gu et al., 2011); to our knowledge, this is the first report of CCYV infecting muskmelon and oriental melon in Korea. Whitefly-transmitted CCYV could present a serious threat of yield losses to cucurbit crops in Korea, requiring control of vector populations to prevent spread of CCYV.

scholarly journals PEWARISAN KETAHANAN MELON (Cucumis melo L.) KULTIVAR MELODI GAMA 3 TERHADAP Kyuri green mottle mosaic virus (RESISTANCE’S INHERITANCE TO Kyuri green mottle mosaic virus IN MELON (Cucumis melo L.) MELODI GAMA 3 CULTIVAR)

2017 ◽  
Vol 20 (2) ◽  
pp. 59 ◽  
Author(s):  
Budi Setiadi Daryono ◽  
Faizatul Fitriyah
Keyword(s):  
Mosaic Virus ◽  
Optical Density ◽  
Immunosorbent Assay ◽  
Cucumis Melo ◽  
Mosaic Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Financial Loss ◽  
Double Antibody ◽  
Cucumis Melo L ◽  
Das Elisa

Melon (Cucumis melo L.) belongs to Cucurbitaceae. Melon has high potential to be developed as main horticultural product in Indonesia. Melon is one of important foreign exchange and is the fifth biggest horticulture commodity in Indonesia. One of the problems in melon farming is mosaic disease caused by Kyuri green mottle mosaic virus (KGMMV). KGMMV infection reduces the quality and the amount of melon production. Melon farmers suffered a significant financial loss. Melodi Gama 3 (MG3) is a high yielding melon cultivar from the Genetics Laboratory, Faculty of Biology, Universitas Gadjah Mada. The use of genetically resistant melon cultivar has beneficial outcome for agriculture sector. The aim of this research was to study the resistance’s inherintance to KGMMV in MG3 melon cultivar. Two cultivars of MG3, MG3|5and MG3|8, were cultivated in the greenhouse. MAI, Glamour, Ladika, and Action melon cultivars were used as references. Resistance of KGMMV was analyzed by symptom observation and serological detection using Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA). DAS-ELISA result analyzed further to establish resistance category. Description to melon cultivar phenotype variation was done. The result of this research indicates that MG3 melon cultivar is tolerant to KGMMV. The decrease of MG3 optical density was directly related with the lowering of KGMMV symptoms. The character of tolerance to KGMMV was inherited from Melodi Gama 1 (MG1) cultivar. Melon (Cucumis melo L.) merupakan tanaman buah yang tergolong dalam familia Cucurbitaceae. Tanaman melon berpotensi untuk dikembangkan sebagai produk unggulan hortikultura di Indonesia. Tanaman melon juga merupakan salah satu penghasil devisa penting Indonesia dan menempati urutan ke-5 dari kelompok hortikultura. Salah satu kendala yang sering dihadapi oleh petani melon adalah penyakit mosaik yang disebabkan oleh Kyuri green mottle mosaic virus (KGMMV). Infeksi KGMMV pada pertanian melon mengakibatkan penurunan kualitas dan kuantitas hasil, sehingga petani mengalami kerugian ekonomi yang cukup berarti. Melodi Gama 3 (MG3) merupakan kultivar melon unggul hasil rakitan Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada. Penggunaan kultivar melon yang tahan terhadap infeksi KGMMV secara genetis merupakan alternatif yang sangat bermanfaat dalam bidang pertanian. Penelitian ini dilakukan untuk mengetahui pewarisan ketahanan MG3 terhadap infeksi KGMMV. Melon kultivar MG3, ditumbuhkan di greenhouse. Sebagai pembanding digunakan melon kultivar yang umum ditanam petani, yaitu MAI, Glamour, Ladika, dan Action. Kelima kultivar melon tersebut diinokulasi dengan KGMMV. Parameter ketahanan KGMMV yang digunakan adalah segregasi gejala dan uji serologis dengan Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA). Hasil DAS-ELISA selanjutnya dianalisis untuk mengetahui kategori ketahanannya. Dilakukan pula deskripsi pada variasi fenotip kultivar melon yang ditanam. Hasil penelitian ini menunjukkan bahwa tanaman melon kultivar Melodi Gama 3 memiliki sifat toleransi terhadap infeksi KGMMV. Toleransi ditunjukkan dengan nilai optical density (OD) yang menurun seiring dengan penurunan gejala infeksi KGMMV. Sifat ketahanan terhadap KGMMV diwariskan dari kultivar Melodi Gama 1 (MG1).

scholarly journals Successful Transfer of the Cucumber Mosaic Virus Coat Protein Gene to Cucumis melo L.

1992 ◽  
Vol 42 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Keiko YOSHIOKA ◽  
Kaoru HANADA ◽  
Yukou NAKAZAKI ◽  
Yuzo MlNOBE ◽  
Toshiro YAKUWA ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Cucumis Melo ◽  
Protein Gene ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Cucumis Melo L ◽  
Successful Transfer
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