Shoot regeneration of hybrid seed geranium (Pelargonium x hortorum) and regal geranium (Pelargonium x domesticum) from primary callus cultures

1989 ◽  
Vol 19 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Kerry B. Dunbar ◽  
Christine T. Stephens
Keyword(s):  
Shoot Regeneration ◽  
Callus Cultures ◽  
Hybrid Seed ◽  
Pelargonium X Hortorum ◽  
Primary Callus ◽  
Pelargonium X Domesticum

scholarly journals IMPROVED PLANT REGENERATION OF SOLANUM AND LYCOPERSICON GENOTYPES FROM LONG-TERM CALLUS CULTURE.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121b-1121
Author(s):  
Chang-Yeon Yu ◽  
John Masiunas
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Callus Cultures ◽  
Solanum Nigrum ◽  
Regeneration Capacity ◽  
Regeneration Medium ◽  
Solanum Ptycanthum ◽  
Shoot Differentiation ◽  
Solanum Nigrum L

Repeated callus sub-culture reduce the regeneration capacity in many species. Our studies determined the effect of genotype and medium on regeneration of several Solanum and Lycopersicon genotypes from long-term callus cultures. In the first study, 13 genotypes were transferred to regeneration medium, including: Murashige and Skoog plus Gamborg Vitamins (MG); Murashige and Skoog (MS); Gamborg (GM); and white (WM). The greatest shoot regeneration was on the MG medium, containing the highest levels of thiamine. Shoot differentiation was greatest with 0.2 mg/l IAA and 2 mg/l BA. No plants were regenerated on GM or WM medium. In a second study, the effect of thiamine (0 to 200 mg/l) on shoot regeneration of the L. peruvianum genotypes PI199380, PI126945, PI251301, and PI128652, along with Solanum ptycanthum, Solanum nigrum, and L. esculentum `Diego' was evaluated. Shoot regeneration of Solanum ptycanthum, Solanum nigrum, L. peruvianum PI 199380 and PI25301 was best with 20 mg/l of thiamine.

scholarly journals In vitro Propagation of Ionidium suffruticosum Ging. ? A Seasonal Multipotent Medicinal Herb.

1970 ◽  
Vol 19 (2) ◽  
pp. 143-150
Author(s):  
Arunkumar B. Sonappanavar ◽  
M. Jayaraj ◽  
Asha N. Bagadekar ◽  
Anant V. Bhandarkar
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Callus Cultures ◽  
Medicinal Herb ◽  
In Vitro Shoots

Indirect regeneration of plant was obtained through organogenesis in leaf callus cultures of Ionidium suffruticosum. Leaf explants were found to be best suited for callus induction on MS with 2, 4-D (0.5 and 1.0 mg/l). Maximum shoot regeneration was obtained in MS supplemented with Kn (4.0 mg/l) alone and NAA (0.4 mg/l) with Kn (2.0 m/l).  The in vitro shoots thus obtained were successfully rooted in MS supplemented with Kn (4.0 mg/l) alone and with NAA (2.0 mg/l) and Kn (0.2  mg/l).  Seventy per cent of the rooted plants survived and they were successfully acclimated in soil. Key words: Ionidium suffruticosum, micropropagation, Medicinal herb D.O.I. 10.3329/ptcb.v19i2.5431 Plant Tissue Cult. & Biotech. 19(2): 143-150, 2009 (December)

scholarly journals Regeneration and Cormels Production of White Prosperity and Priscilla Gladiolus Varities In Vitro

2009 ◽  
Vol 6 (3) ◽  
pp. 432-441
Author(s):  
Baghdad Science Journal
Keyword(s):  
Shoot Regeneration ◽  
Callus Cultures ◽  
Peat Moss ◽  
Naphthalene Acetic Acid ◽  
Root Number ◽  
Benzyl Adenine ◽  
River Sand ◽  
Callus Initiation

Plant regeneration and cormel production was carried out from callus cultures initiated from White Prosperity and Priscilla Gladiolus Varities. It is aimed to produce plants and cormels in vitro all year round. The study included many experiments, these were the effect of Naphthalene acetic acid (NAA) and Kinetin (Kin) interaction on callus initiation, effect of Benzyl adenine (BA) on shoot regeneration from callus culture, effect of NAA on rooting after 30, 40 and 50 days in culture. The role of the type of agricultural medium (Peat moss or river sand and their mixture on plantlets survival after weaning was studied. Results showed that the interaction between NAA and Kin induced callus on axillary bud explants. Callus was best initiated by using a combination (10.0, 0.5) mg/l for White Prosperity, (0.5, 1.0) and (10.0, 0.5) mgl for Priscilla of NAA and Kin respectively. Regeneration for the two varieties was best occurred when media were supplemented with BA at 1.0 mg/l achieving maximum number of shoots (6.2) and height (4.96 cm.). Highest response for shoot regeneration from callus occurred at a concentration of 0.5 mg/l NAA reached 100% and 83.3% for White Prosperity and Priscilla respectively. An obvious increase in rooting percentage, root number and length over time. Both varieties showed 100% response for cormels formation 50 days after rooting. Plantlets are well established in peat moss.

Sign in / Sign up

Export Citation Format

Share Document