In vitro plantlet regeneration of Ocotea catharinensis, an endangered Brazilian hardwood forest tree

1993 ◽  
Vol 35 (3) ◽  
pp. 279-286 ◽  
Author(s):  
P. H. Moura-Costa ◽  
A. M. Viana ◽  
S. H. Mantell
Keyword(s):  
Forest Tree ◽  
Plantlet Regeneration ◽  
Hardwood Forest

scholarly journals In vitro multiplication of Cedrela fissilis Vell.: A threatened brazilian hardwood forest tree

2021 ◽  
Vol 17 ◽  
pp. 1-11
Author(s):  
Yasmim Smarsaro Bonfá ◽  
Vitor de Laia Nascimento ◽  
Elias Terra Werner
Keyword(s):  
Forest Tree ◽  
Hardwood Forest ◽  
In Vitro Multiplication

scholarly journals In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

2014 ◽  
Vol 8 (24) ◽  
pp. 847-856
Author(s):  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
Gopal Ghanta Rama ◽  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
...  
Keyword(s):  
Callus Induction ◽  
Plantlet Regeneration

In vitro plantlet regeneration of Olea europaea ssp. maderensis

2003 ◽  
Vol 97 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Conceição V. Santos ◽  
Gina Brito ◽  
Gloria Pinto ◽  
Henrique M.A.C. Fonseca
Keyword(s):  
Olea Europaea ◽  
Plantlet Regeneration ◽  
Olea Europaéa

scholarly journals In vitro regeneration performance of Corchorus olitorius

1970 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
M Hoque ◽  
KM Nasiruddin ◽  
GKMN Haque ◽  
GC Biswas
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Root Formation ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Corchorus Olitorius ◽  
The Media ◽  
Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

scholarly journals Naphthenic acids - alternative rooting stimulators in black locust microshoots

2014 ◽  
pp. 7-13
Author(s):  
Branislav Kovacevic ◽  
Slavko Kevresan ◽  
Sasa Orlovic ◽  
Dragana Miladinovic ◽  
Vera Cirin-Novta ◽  
...  
Keyword(s):  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Forest Tree ◽  
Naphthenic Acids ◽  
Control Treatment ◽  
In Vitro Cultivation ◽  
Test Treatments ◽  
Robinia Pseudoacacia L ◽  
Positive Effect

The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type ?Velebit? from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 ?M of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole- 3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 ?M of IBA. However, treatment with 10 ?M of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings.

scholarly journals Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 248
Author(s):  
Barry Flinn ◽  
Savanah Dale ◽  
Andrew Disharoon ◽  
Stephen Kresovich
Keyword(s):  
Plantlet Regeneration ◽  
Induction Phase ◽  
Genetic Studies ◽  
Response Profiles ◽  
The Common ◽  
Parental Lines ◽  
Sorghum Genotypes ◽  
Better Than

Sorghum has been considered a recalcitrant plant in vitro and suffers from a lack of regeneration protocols that function broadly and efficiently across a range of genotypes. This study was initiated to identify differential genotype-in vitro protocol responses across a range of bioenergy sorghum parental lines and the common grain sorghum genotype Tx430 in order to characterize response profiles for use in future genetic studies. Two different in vitro protocols, LG and WU, were used for comparisons. Distinct genotype-protocol responses were observed, and the WU protocol performed significantly better for plantlet regeneration. Most bioenergy genotypes performed as well, if not better than Tx430, with Rio and PI329311 as the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic exudation responses, as indicated by medium browning. During the callus induction phase, genotypes prone to medium browning exhibited a response on WU medium which was either equal or greater than on LG medium. Genotype- and protocol-dependent albino plantlet regeneration was also noted, with three of the bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were susceptible to albino plantlet regeneration, with the response strongly associated with the WU protocol. These bioenergy parental genotypes, and their differential responses under two in vitro protocols, provide tools to further explore and assess the role of genetic loci, candidate genes, and allelic variants in the regulation of in vitro responsiveness in sorghum.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

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