Site-directed mutagenesis and expression inEscherichia coli of WMAI-1, a wheat monomeric inhibitor of insect ?-amylase

1991 ◽  
Vol 17 (5) ◽  
pp. 1005-1011 ◽  
Author(s):  
Federico Garc�a-Maroto ◽  
Pilar Carbonero ◽  
Francisco GArc�a-Olmedo
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Inescherichia Coli ◽  
Expression Inescherichia Coli

scholarly journals Two Amino Acid Residues of Transposase Contributing to Differential Transposability of IS1 Elements inEscherichia coli

1998 ◽  
Vol 180 (19) ◽  
pp. 5279-5283 ◽  
Author(s):  
Jiann-Hwa Chen ◽  
Wen-Ben Hsu ◽  
Jiing-Luen Hwang
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Inescherichia Coli ◽  
Amino Acid Residues ◽  
Insertion Element ◽  
Resistance Plasmid ◽  
Insertion Mutants

ABSTRACT Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspection of the transposase sequences of the four IS1 types and the IS1 of the resistance plasmid R100 showed that two amino acid residues, His-193 and Leu-217 of transposase, might contribute to differential transposability of IS1 elements in W3110. The two amino acid residues of the transposase in IS1A (IS1E and IS1G) were altered separately by site-directed mutagenesis, and each mutant was found to mediate transposition at a frequency about 30-fold lower than that of IS1A (IS1E and IS1G). Thus, the assumption that His-193 and Leu-217 of transposase contribute to differential transposability of IS1 elements in W3110 was confirmed.

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Site-directed mutagenesis on alkaline phosphatasefrompseudomonas aeruginosapao1 for enhanced activity

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
UMA SELVARAJ ◽  
THIRUMALAI MUTHUKUMARESAN ◽  
GAYATHRI VIJAYENDRAN ◽  
SENTHIL KUMAR DEVAN ◽  
VENU BABU P ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enhanced Activity
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