Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ-related alteration of NDK activities during rice seed germination (Oryza sativa L.)

1995 ◽  
Vol 27 (5) ◽  
pp. 1053-1058 ◽  
Author(s):  
Akira Yano ◽  
Masaaki Umeda ◽  
Hirofumi Uchimiya
Keyword(s):  
Escherichia Coli ◽  
Oryza Sativa ◽  
Seed Germination ◽  
Oryza Sativa L ◽  
Nucleoside Diphosphate Kinase ◽  
Rice Seed

scholarly journals Germination and seedling growth of rice (Oryza sativa L.) as affected by varying concentrations of loom-dye effluent

2019 ◽  
Vol 17 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Md. Arifur Rahman ◽  
Md. Sohanur Rahman ◽  
K.M. Mohiuddin ◽  
Md. Akhter Hossain Chowdhury ◽  
Md. Abul Khair Chowdhury
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Seedling Growth ◽  
Oryza Sativa L ◽  
Industrial Effluent ◽  
Rice Seed ◽  
Early Seedling Growth ◽  
Significant Difference ◽  
Early Seedling ◽  
Radical Length

Indiscriminate discharge of industrial effluent has become a serious problem for the agro-ecological environment in most of the areas of Bangladesh. The effects of loom-dye effluents on seed germination and early seedling growth of rice (Oryza sativa L.) were investigated by conducting an experiment in the laboratory of the Department of Agricultural Chemistry of Bangladesh Agricultural University, Mymensingh. Three types of loom-dye effluents were applied in sterilized petridishes at different loading ratios. Seven treatments (i.e., T0, T1, T2, T3, T4, T5 and T6 with 0, 5, 10, 25, 50, 75 and 100% effluent, respectively) of each effluent were used following completely randomized design (CRD) and replicated three times. Subsequently, Pb, Fe, Zn, Mn and Cr accumulation were also investigated in the harvested rice seedlings. Rice seed showed a significant difference in germination percentage with varying levels of effluent application at different days after setting of germination. The decreased seed germination rate and seedling growth of rice were observed with increased concentration of loom-dye effluents. The highest germination speed (97.8%) was obtained from control whereas the lowest germination speeds were obtained from T5 of black, and T6 of both black and violet effluents. Phytotoxic effects of loom-dye effluents on germination and radical length were extreme at 100% effluent concentration having the order of black > violet > pink. The maximum radical length (6.4 cm) and plumule length (7.5 cm) were observed with T1 of pink dye effluent whereas the minimum length of radical and plumule were obtained from T6 treatment of the effluents. The highest fresh weight (39.8 mg petridish–1), dry weight (5.7 mg petridish–1) and seedling vigor index (746.7%) were also observed from T1 of pink dye effluent. The maximum uptake of Pb, Fe, Mn and Zn was 0.48, 3.81, 0.79 and 0.13 μg g−1, respectively. The uptake of Cr was below the detectable limit. Total heavy metal accumulation in rice was in the following order: Fe>Mn>Pb>Zn>Cr. Results showed that the higher concentration of loom-dye effluent showed the higher toxic effects on different parameters of germination and early seedling growth compared with the lower effluent concentrations. J. Bangladesh Agril. Univ. 17(2): 153–160, June 2019

Allelopathic Potential of Rice Seed (Oryza sativa L.) on Seed Germination of Monochoria vaginalis var. plantaginea.

1997 ◽  
Vol 42 (3) ◽  
pp. 262-267 ◽  
Author(s):  
Shun Kawaguchi ◽  
Yasutomo Takeuchi ◽  
Masaru Ogasawara ◽  
Koichi Yoneyama ◽  
Makoto Konnai
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Oryza Sativa L ◽  
Rice Seed ◽  
Allelopathic Potential

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

Changes in Cytokinin Activity during Seed Germination in Rice (Oryza sativa L.)

1984 ◽  
Vol 54 (1) ◽  
pp. 1-5 ◽  
Author(s):  
SHYAMALI SAHA ◽  
P. K. NAGAR ◽  
P. K. SIRCAR
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Oryza Sativa L ◽  
Cytokinin Activity

scholarly journals Mechanism of delayed seed germination caused by high temperature during grain filling in rice (Oryza sativa L.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chetphilin Suriyasak ◽  
Yui Oyama ◽  
Toshiaki Ishida ◽  
Kiyoshi Mashiguchi ◽  
Shinjiro Yamaguchi ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
High Temperature ◽  
Seed Germination ◽  
Oryza Sativa L ◽  
Grain Filling ◽  
Aleurone Layer ◽  
Gene Promoters ◽  
Aba Catabolism ◽  
Amylase Genes ◽  
Transcriptional Changes

Abstract High temperature during grain filling considerably reduces yield and quality in rice (Oryza sativa L.); however, how high temperature affects seed germination of the next generation is not yet well understood. Here, we report that seeds from plants exposed to high temperature during the grain filling stage germinated significantly later than seeds from unstressed plants. This delay remained even after dormancy release treatments, suggesting that it was not due to primary seed dormancy determined during grain filling. In imbibed embryos of heat-stressed seeds, expression of abscisic acid (ABA) biosynthesis genes (OsNCEDs) was higher than in those of control seeds, whereas that of ABA catabolism genes (OsABA8′OHs) was lower. In the aleurone layer, despite no change in GA signaling as evidenced by no effect of heat stress on OsGAMYB gene expression, the transcripts of α-amylase genes OsAmy1C, OsAmy3B, and OsAmy3E were significantly down-regulated in heat-stressed seeds in comparison with controls. Changes in promoter methylation levels were consistent with transcriptional changes of ABA catabolism-related and α-amylase genes. These data suggest that high temperature during grain filling results in DNA methylation of ABA catabolism-related and α-amylase gene promoters, delaying germination of heat-stressed seeds.

scholarly journals Cyclin-Dependent Kinase Inhibitors KRP1 and KRP2 Are Involved in Grain Filling and Seed Germination in Rice (Oryza sativa L.)

2019 ◽  
Vol 21 (1) ◽  
pp. 245 ◽  
Author(s):  
Abolore Adijat Ajadi ◽  
Xiaohong Tong ◽  
Huimei Wang ◽  
Juan Zhao ◽  
Liqun Tang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Cellular Localization ◽  
Kinase Inhibitors ◽  
Oryza Sativa L ◽  
Grain Filling ◽  
Rice Seed ◽  
Cyclin Dependent Kinase ◽  
Cell Cycles ◽  
Transgenic Lines ◽  
Dependent Protein

Cyclin-dependent kinase inhibitors known as KRPs (kip-related proteins) control the progression of plant cell cycles and modulate various plant developmental processes. However, the function of KRPs in rice remains largely unknown. In this study, two rice KRPs members, KRP1 and KRP2, were found to be predominantly expressed in developing seeds and were significantly induced by exogenous abscisic acid (ABA) and Brassinosteroid (BR) applications. Sub-cellular localization experiments showed that KRP1 was mainly localized in the nucleus of rice protoplasts. KRP1 overexpression transgenic lines (OxKRP1), krp2 single mutant (crkrp2), and krp1/krp2 double mutant (crkrp1/krp2) all exhibited significantly smaller seed width, seed length, and reduced grain weight, with impaired seed germination and retarded early seedling growth, suggesting that disturbing the normal steady state of KRP1 or KRP2 blocks seed development partly through inhibiting cell proliferation and enlargement during grain filling and seed germination. Furthermore, two cyclin-dependent protein kinases, CDKC;2 and CDKF;3, could interact with KRP1 in a yeast-two-hybrid system, indicating that KRP1 might regulate the mitosis cell cycle and endoreduplication through the two targets. In a word, this study shed novel insights into the regulatory roles of KRPs in rice seed maturation and germination.

The submergence-induced gene OsCTP in rice (Oryza sativa L.) is similar to Escherichia coli cation transport protein ChaC

Plant Science ◽  
2005 ◽  
Vol 168 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Yanhua Qi ◽  
Yasuo Yamauchi ◽  
Jianqun Ling ◽  
Naoyoshi Kawano ◽  
Debao Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Cation Transport ◽  
Transport Protein
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