Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum

1995 ◽  
Vol 27 (5) ◽  
pp. 1037-1042 ◽  
Author(s):  
Faustino Merch�n ◽  
Rafael Prieto ◽  
Karen L. Kindle ◽  
Mar�a J. Llama ◽  
Juan L. Serra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nitrite Reductase ◽  
Phormidium Laminosum ◽  
Expression In Escherichia Coli ◽  
Thermophilic Cyanobacterium ◽  
Nitrite Reductase Gene ◽  
Reductase Gene

Molecular Cloning and Characterization of Nitrite Reductase Gene from Sugarbeet

2011 ◽  
Vol 37 (8) ◽  
pp. 1406-1414
Author(s):  
Xiao-Yan SHI ◽  
Yan-Da ZENG ◽  
Shi-Long LI ◽  
Yu-Bo WANG ◽  
Feng-Ming MA ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Nitrite Reductase ◽  
Nitrite Reductase Gene ◽  
Reductase Gene

scholarly journals A novel nitrite reductase gene from the cyanobacterium Plectonema boryanum.

1995 ◽  
Vol 177 (21) ◽  
pp. 6137-6143 ◽  
Author(s):  
I Suzuki ◽  
H Kikuchi ◽  
S Nakanishi ◽  
Y Fujita ◽  
T Sugiyama ◽  
...  
Keyword(s):  
Nitrite Reductase ◽  
Plectonema Boryanum ◽  
Nitrite Reductase Gene ◽  
Reductase Gene

scholarly journals Denitrification Activity and Relevant Bacteria Revealed by Nitrite Reductase Gene Fragments in Soil of Temperate Mixed Forest

2009 ◽  
Vol 24 (1) ◽  
pp. 76
Author(s):  
Chie Katsuyama ◽  
Naho Kondo ◽  
Yuichi Suwa ◽  
Takao Yamagishi ◽  
Masayuki Itoh ◽  
...  
Keyword(s):  
Nitrite Reductase ◽  
Mixed Forest ◽  
Nitrite Reductase Gene ◽  
Reductase Gene ◽  
Denitrification Activity ◽  
Temperate Mixed Forest

Agricultural soil denitrifiers possess extensive nitrite reductase gene diversity

2017 ◽  
Vol 19 (3) ◽  
pp. 1189-1208 ◽  
Author(s):  
Sara Coyotzi ◽  
Andrew C. Doxey ◽  
Ian D. Clark ◽  
David R. Lapen ◽  
Philippe Van Cappellen ◽  
...  
Keyword(s):  
Nitrite Reductase ◽  
Agricultural Soil ◽  
Gene Diversity ◽  
Nitrite Reductase Gene ◽  
Reductase Gene

scholarly journals Cloning of a Novel Aldo-Keto Reductase Gene from Klebsiella sp. Strain F51-1-2 and Its Functional Expression in Escherichia coli

2007 ◽  
Vol 73 (15) ◽  
pp. 4959-4965 ◽  
Author(s):  
Hong Jiang ◽  
Chao Yang ◽  
Hong Qu ◽  
Zheng Liu ◽  
Q. S. Fu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Organophosphorus Compounds ◽  
Functional Expression ◽  
Nitrilotriacetic Acid ◽  
Coding Region ◽  
Reductive Transformation ◽  
Cofactor Specificity ◽  
Expression In Escherichia Coli ◽  
Reductase Gene ◽  
One Step

ABSTRACT A soil bacterium capable of metabolizing organophosphorus compounds by reducing the P═S group in the molecules was taxonomically identified as Klebsiella sp. strain F51-1-2. The gene involved in the reduction of organophosphorus compounds was cloned from this strain by the shotgun technique, and the deduced protein (named AKR5F1) showed homology to members of the aldo-keto reductase (AKR) superfamily. The intact coding region for AKR5F1 was subcloned into vector pET28a and overexpressed in Escherichia coli BL21(DE3). Recombinant His6-tagged AKR5F1 was purified in one step using Ni-nitrilotriacetic acid affinity chromatography. Assays for cofactor specificity indicated that reductive transformation of organophosphorus compounds by the recombinant AKR5F1 specifically required NADH. The kinetic constants of the purified recombinant AKR5F1 toward six thion organophosphorus compounds were determined. For example, the Km and k cat values of reductive transformation of malathion by the purified recombinant AKR5F1 are 269.5 ± 47.0 μΜ and 25.7 ± 1.7 min−1, respectively. Furthermore, the reductive transformation of organophosphorus compounds can be largely explained by structural modeling.

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