A linear DNA molecule of 5.9 kilobase-pairs is highly homologous to the chloroplast DNA in the green alga Chlamydomonas moewusii

1986 ◽  
Vol 6 (5) ◽  
pp. 313-319 ◽  
Author(s):  
Monique Turmel ◽  
Guy Bellemare ◽  
Robert W. Lee ◽  
Claude Lemieux
Keyword(s):  
Chloroplast Dna ◽  
Green Alga ◽  
Linear Dna ◽  
Dna Molecule ◽  
Chlamydomonas Moewusii

scholarly journals Purification, Visualization and Characterization of the Sexual Agglutinins of the Green Alga Chlamydomonas moewusii yapensis

Microbiology ◽  
1987 ◽  
Vol 133 (11) ◽  
pp. 3183-3191 ◽  
Author(s):  
M. R. Samson ◽  
F. M. Klis ◽  
K. J. Crabbendam ◽  
P. Van Egmond ◽  
H. Van Den Ende
Keyword(s):  
Green Alga ◽  
Chlamydomonas Moewusii ◽  
Sexual Agglutinins

DNA supercoiling and transcription: topological coupling of promoters

1996 ◽  
Vol 29 (3) ◽  
pp. 203-225 ◽  
Author(s):  
David M. J. Lilley ◽  
Dongrong Chen ◽  
Richard P. Bowater
Keyword(s):  
Fundamental Property ◽  
Dna Supercoiling ◽  
The Other ◽  
Linear Dna ◽  
Dna Molecule ◽  
Linking Number ◽  
Closed Circle ◽  
A Chain

DNA supercoiling is a consequence of the double-stranded nature of DNA. When a linear DNA molecule is ligated into a covalently closed circle, the two strands become intertwined like the links of a chain, and will remain so unless one of the strands is broken. The number of times one strand is linked with the other is described by a fundamental property of DNA supercoiling, the linking number (Lk).

scholarly journals Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.

1983 ◽  
Vol 3 (9) ◽  
pp. 1562-1566 ◽  
Author(s):  
K G Murti ◽  
D M Prescott
Keyword(s):  
Electron Microscopy ◽  
S Phase ◽  
Origin Of Replication ◽  
Dna Molecules ◽  
Linear Dna ◽  
Dna Molecule ◽  
Independent Unit

Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed: (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that: (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.

The lengths of fragments covering a fixed marker in randomly cut linear or circular DNA

1979 ◽  
Vol 16 (04) ◽  
pp. 721-731
Author(s):  
Samuel Litwin
Keyword(s):  
Fragment Length ◽  
Random Number ◽  
Length Distribution ◽  
Attachment Site ◽  
Jump Discontinuity ◽  
Circular Dna ◽  
Linear Dna ◽  
Dna Molecule ◽  
Recovery Pattern

A linear DNA molecule may be labelled at a fixed locus by a minute complementary radioactive molecule. A collection of identical molecules is to be so labelled and each one independently cut at a random number, N, of random places, X (N Poisson, X uniform). Fragments containing the label are to be collected and assayed by length. It is shown that the recovery pattern (fragment length distribution) contains a jump discontinuity at the fixed locus and may be used to determine the distance between the attachment site and the nearest end of the molecule. The recovery pattern under the hypothesis that the collection of molecules are circularly permuted, i.e. the labelled locus is uniformly distributed over the length of the molecule, does not contain such discontinuities. The case where the labelling molecule has a non-negligible extent is also treated.

The lengths of fragments covering a fixed marker in randomly cut linear or circular DNA

1979 ◽  
Vol 16 (4) ◽  
pp. 721-731
Author(s):  
Samuel Litwin
Keyword(s):  
Fragment Length ◽  
Random Number ◽  
Length Distribution ◽  
Attachment Site ◽  
Jump Discontinuity ◽  
Circular Dna ◽  
Linear Dna ◽  
Dna Molecule ◽  
Recovery Pattern

A linear DNA molecule may be labelled at a fixed locus by a minute complementary radioactive molecule. A collection of identical molecules is to be so labelled and each one independently cut at a random number, N, of random places, X (N Poisson, X uniform). Fragments containing the label are to be collected and assayed by length. It is shown that the recovery pattern (fragment length distribution) contains a jump discontinuity at the fixed locus and may be used to determine the distance between the attachment site and the nearest end of the molecule.The recovery pattern under the hypothesis that the collection of molecules are circularly permuted, i.e. the labelled locus is uniformly distributed over the length of the molecule, does not contain such discontinuities. The case where the labelling molecule has a non-negligible extent is also treated.

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