Photoautotrophic growth of soybean cells in suspension culture. II. Optimization of culture medium and conditions

1992 ◽  
Vol 30 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Michael E. Horn ◽  
Barry A. Martin ◽  
Jack M. Widholm
Keyword(s):  
Suspension Culture ◽  
Culture Medium ◽  
Photoautotrophic Growth

scholarly journals Handling culture medium composition for optimizing plant cell suspension culture in shake flasks

2017 ◽  
Vol 133 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Tiago Fidemann ◽  
Gabriela Aparecida de Araujo Pereira ◽  
Tárik Reis Heluy ◽  
Rodrigo Boccoli Gallego ◽  
Mônica Rosa Bertão ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Culture Medium ◽  
Medium Composition ◽  
Plant Cell Suspension Culture ◽  
Plant Cell Suspension ◽  
Shake Flasks

scholarly journals The Effect of Pyridinecarbothioamides on Isoflavonoid Production in Genista tinctoria Cultures in Vitro

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800
Author(s):  
Lenka Tůmová ◽  
Věra Klimešová ◽  
Anna Vildová
Keyword(s):  
Suspension Culture ◽  
Callus Culture ◽  
Culture Medium ◽  
Culture In Vitro ◽  
Elicitor Treatment ◽  
Pyrazinecarboxylic Acid ◽  
Genista Tinctoria ◽  
Derivatives Of ◽  
Maximum Content

Previous studies showed that derivatives of pyrazinecarboxylic acid used as elicitors increased either flavonoid or flavonolignan production. The effect of 2-(3-bromobenzylsulfanyl) pyridine-4-carbothioamide on isoflavonoids production in Genista tinctoria culture in vitro was tested. The highest isoflavonoid content (0.3 mg.g−1 DW) in callus culture was found after elicitor application at a concentration of 2.96 × 10−5 mol.L−1 after 24 h of treatment. The maximum daidzein production (0.2 mg.g−1 DW) was detected after 48 h of elicitor treatment at a concentration of 2.96 × 10−3 mol.L-1 and after 24 h of elicitor treatment at a concentration of 2.96 × 10−5 mol.L−1. Suspension culture produced the highest amount of isoflavonoids (0.3 mg.g−1 DW) after elicitor application at concentrations of 2.96 × 10−4 mol.L−1 after 24 and 168 h of treatment, and 2.96 × 10−5 mol.L−1 after 168 h of treatment. The highest content of genistin (0.3 mg. g−1 DW) was detected after 24 h of elicitor treatment at a concentration of 2.96 × 10−4 mol.L−1. The maximum content of daidzein (3.80 mg.L−1) released into the suspension culture medium was found after elicitor application at a concentration of 2.96 × 10−4 mol.L−1 after 12 h of treatment.

scholarly journals Enhanced Rosmarinic Acid Production by Lavandula vera MM Cell Suspension Culture through Elicitation with Vanadyl Sulfate

2006 ◽  
Vol 61 (3-4) ◽  
pp. 241-244 ◽  
Author(s):  
Milen Georgiev ◽  
Sonya Kuzeva ◽  
Atanas Pavlov ◽  
Elena Kovacheva ◽  
Mladenka Ilieva
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Culture Medium ◽  
Vanadyl Sulfate ◽  
Lavandula Vera Mm ◽  
Intracellular Content ◽  
Abiotic Elicitor

The influence of elicitation on rosmarinic acid biosynthesis by Lavandula vera MM cell suspension culture was investigated using vanadyl sulfate as an abiotic elicitor. It was established that 12 h after treatment with 25 mg/l vanadyl sulfate the rosmarinic acid production was increased up to 3.92 g/l (2.8 times higher compared to the control cultivation). No significant amounts of rosmarinic acid were detected in the culture medium in comparison with its intracellular content. However, it was observed that the extracellular content of rosmarinic acid is 3.3 times higher compared to the control variant (4 h after treatment at elicitor concentration 25 mg/l).

scholarly journals Determination of Salt Tolerance of Oil Palm SUP-PSU 1 Using Cell Suspension Culture

2021 ◽  
Author(s):  
Thirasak Sukdee ◽  
Sompong Te-chato ◽  
SUREERAT YENCHON
Keyword(s):  
Electrical Conductivity ◽  
Growth Rate ◽  
Suspension Culture ◽  
Oil Palm ◽  
Culture Medium ◽  
Proline Content ◽  
Repeated Application ◽  
Fresh Weight

Abstract Oil palm is an oil crop that produces the highest oil yield throughout the year. Repeated application of fertilizer in oil palm orchard results in severe salinity of planting areas. Therefore, the objective of this study was to determine growth, physiochemistry and re-growth of calli as effected by sodium chloride (NaCl). The calli were cultured in liquid oil palm culture medium (OPCM) supplemented with different concentrations of NaCl (0, 50, 100, 150, 200, 300 and 400 mM), 0.1 mg/l dicamba and 200 mg/l ascorbic acid for 4, 8 and 12 weeks. The results showed that increase in concentrations of NaCl and exposure times decreased growth rate of cells in suspension culture. The concentration of NaCl which inhibited growth rate at 50 % (IC50) was 166.73 mM after culture for 12 weeks. Increase in concentrations of NaCl and exposure times increased electrolyte leakage (EL), changing of electrical conductivity (EC) medium and proline content. NaCl at concentration of 400 mM gave the highest EL, changing of EC medium and proline content at 82.22 %, 3.77 mS/cm and 64.93 µg/gFW, respectively. Over 200 mM NaCl oil palm cells cannot survive. After transfer treated callus clumps to OPCM without NaCl for 8 weeks, the clumps treated in 50 mM NaCl gave the highest fresh weight at 416.5 mg while 100 mM gave the highest average number of nodules per tube at 84 nodules. This result suggests that cells of oil palm in vitro could not stand to NaCl at concentration higher than 200 mM.

scholarly journals Changes in auxin level in the course of growth of a sunflower crown-gall suspension culture

2014 ◽  
Vol 52 (3-4) ◽  
pp. 285-294 ◽  
Author(s):  
Zofia Chirek
Keyword(s):  
Suspension Culture ◽  
Crown Gall ◽  
Culture Medium ◽  
Cell Mass ◽  
Maximum Growth ◽  
Maximal Growth ◽  
Auxin Level ◽  
Culture Cycle ◽  
Crown Gall Tumour ◽  
Further Development

The auxin level in the cell mass and culture medium was determined by means of the <em>Avena</em> straight caleoptile test in various periods of the suspension culture cycle of the sunflower crown-gall tumour. The investigations were performed in the course of the zero passage (PO) and first one (Pl), differing in their time of duration of maximum growth and its intensity. In both passages the intra- and extra-cellular auxin levels reach values of the same order. At the beginning of the maximal growth phase the activity corresponding to IAA in the cells prevails over that of the other auxin-like compounds. This disproportion diminishes with further development of the culture, and with the beginning of the stationary phase the cellular IAA level is lower than that of the remaining auxin-like compounds. The short phase of maximal growth (PO) occurs with an auxin level decreasing in the cell mass and increasing in the medium, and towards the end of the cycle these levels become equal. During the long phase of maximal growth (Pl) the total amount of auxins in the cells increases and is 2-3 times higher than in the medium, whereas IAA in the cells remains at a constant level. These results suggest that the participation of IAA in the intracellular pool of auxin-like substances is decisive for the mitotic activity of the cells and maintenance of growth in the culture.

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