Overcoming the self-incompatibility of Lilium longiflorum Thunb. by application of flower-organ extract or temperature treatment of pollen

Euphytica ◽  
1981 ◽  
Vol 30 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Sachiko Matsubara
Keyword(s):  
Lilium Longiflorum ◽  
Temperature Treatment ◽  
The Self ◽  
Self Incompatibility ◽  
Flower Organ ◽  
Organ Extract

STUDIES ON THE SELF-INCOMPATIBILITY RESPONSE OF LILIUM LONGIFLORUM

1976 ◽  
Vol 63 (8) ◽  
pp. 1104-1108 ◽  
Author(s):  
William F. Fett ◽  
Jack D. Paxton ◽  
David B. Dickinson
Keyword(s):  
Lilium Longiflorum ◽  
The Self ◽  
Self Incompatibility

Pollen‒pistil interaction in Lilium longiflorum : the role of the pistil in controlling pollen tube growth following cross- and self-pollinations

1982 ◽  
Vol 215 (1198) ◽  
pp. 45-62 ◽  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Active Component ◽  
Lilium Longiflorum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
System A ◽  
Pollen Tube Elongation

By cytophysiological methods, the self-incompatibility mechanism of the breeding system in Lilium longiflorum has been examined with particular reference to the synthesis, location and nature of the stylar factors involved in the control of pollen tube development. A ‘bioassay’ has been developed by which the effect of stylar extracts on pollen tube elongation may be investigated. With use of this system, a crude fraction of proteins from the stylar fluid has been shown to inhibit pollen tube growth only when protein fractions from ‘self’ styles are used. The proteins of this fraction have been analysed by thin-layer gel electrofocusing. Changes in the profiles thus obtained following selfing and a heat treatment known to inactivate the self-incompatibility response indicate a highly polarized glycoprotein to be an active component of the system. The various ways by which such a glycoprotein could control pollen tube elongation are considered in detail, and these events in Lilium are discussed in the light of our knowledge of other self-incompatibility systems operating in angiosperms.

scholarly journals Relationship between the Self-Incompatibility and cAMP Level in Lilium longiflorum

2001 ◽  
Vol 42 (11) ◽  
pp. 1234-1238 ◽  
Author(s):  
Akiko Tsuruhara ◽  
Takafumi Tezuka
Keyword(s):  
Lilium Longiflorum ◽  
Camp Level ◽  
The Self ◽  
Self Incompatibility

scholarly journals Molecular characteristics of S-RNase alleles as the determinant of self-incompatibility in the style of Fragaria viridis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianke Du ◽  
Chunfeng Ge ◽  
Tingting Li ◽  
Sanhong Wang ◽  
Zhihong Gao ◽  
...  
Keyword(s):  
Diploid Species ◽  
The Self ◽  
Molecular Characteristics ◽  
Self Incompatibility ◽  
Intraspecific Hybridization ◽  
Noncoding Sequences ◽  
Family Analysis ◽  
The Family ◽  
Genotype Identification ◽  
Rnase T2

AbstractStrawberry (Fragaria spp.) is a member of the Rosoideae subfamily in the family Rosaceae. The self-incompatibility (SI) of some diploid species is a key agronomic trait that acts as a basic pollination barrier; however, the genetic mechanism underlying SI control in strawberry remains unclear. Two candidate S-RNases (Sa- and Sb-RNase) identified in the transcriptome of the styles of the self-incompatible Fragaria viridis 42 were confirmed to be SI determinants at the S locus following genotype identification and intraspecific hybridization using selfing progenies. Whole-genome collinearity and RNase T2 family analysis revealed that only an S locus exists in Fragaria; however, none of the compatible species contained S-RNase. Although the results of interspecific hybridization experiments showed that F. viridis (SI) styles could accept pollen from F. mandshurica (self-compatible), the reciprocal cross was incompatible. Sa and Sb-RNase contain large introns, and their noncoding sequences (promotors and introns) can be transcribed into long noncoding RNAs (lncRNAs). Overall, the genus Fragaria exhibits S-RNase-based gametophytic SI, and S-RNase loss occurs at the S locus of compatible germplasms. In addition, a type of SI-independent unilateral incompatibility exists between compatible and incompatible Fragaria species. Furthermore, the large introns and neighboring lncRNAs in S-RNase in Fragaria could offer clues about S-RNase expression strategies.

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

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