The effects of gibberellic acid and abscisic acid on ?-amylase mRNA levels in barley aleurone layers studies using an ?-amylase cDNA clone

1984 ◽  
Vol 3 (6) ◽  
pp. 407-418 ◽  
Author(s):  
Peter M. Chandler ◽  
John A. Zwar ◽  
John V. Jacobsen ◽  
Thomas J. V. Higgins ◽  
Adam S. Inglis
1982 ◽  
Vol 1 (3) ◽  
pp. 191-215 ◽  
Author(s):  
Thomas J. V. Higgins ◽  
John V. Jacobsen ◽  
John A. Zwar

1984 ◽  
Vol 62 (6) ◽  
pp. 1245-1249 ◽  
Author(s):  
L. S. Kott ◽  
K. J. Kasha

Somatic embryogenesis was induced in callus previously initiated from immature embryos of barley. These cultures ranged in age from 6 weeks to 30 months. Embryoids were readily initiated from homogenized suspension-grown aggregates when plated on modified B5 media with 2,4-dichlorophenoxyacetic acid. Low concentrations (0.1 and 0.05 mg∙L−1) of abscisic acid promoted further maturation of embryoids, while gibberellic acid (1 mg∙L−1) and kinetin (0.1 mg∙L−1) were used in the media to encourage embryoid germination. The development of somatic embryoids from initiation through maturation and germination is described.


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