Adenine depurination and inactivation of plant ribosomes by an antiviral protein of Mirabilis jalapa (MAP)

1992 ◽  
Vol 20 (6) ◽  
pp. 1111-1119 ◽  
Author(s):  
Jiro Kataoka ◽  
Noriyuki Habuka ◽  
Masashi Miyano ◽  
Chikara Masuta ◽  
Akira Koiwai
Keyword(s):  
Antiviral Protein ◽  
Mirabilis Jalapa ◽  
Plant Ribosomes

scholarly journals Antiviral and Antiviroid Activity of MAP-Containing Extracts from Mirabilis jalapa Roots

Plant Disease ◽  
1999 ◽  
Vol 83 (12) ◽  
pp. 1116-1121 ◽  
Author(s):  
Jorge M. Vivanco ◽  
Maddalena Querci ◽  
Luis F. Salazar
Keyword(s):  
Potato Virus ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Antiviral Effect ◽  
Potato Spindle Tuber Viroid ◽  
Antiviral Protein ◽  
Potato Leaf ◽  
Mirabilis Jalapa ◽  
Spot Hybridization ◽  
Mirabilis Antiviral Protein

Extracts of Mirabilis jalapa (Nyctaginaceae), containing a ribosome inactivating protein (RIP) called Mirabilis antiviral protein (MAP), were tested against infection by potato virus X, potato virus Y, potato leaf roll virus, and potato spindle tuber viroid. Root extracts of M. jalapa sprayed on test plants 24 h before virus or viroid inoculation inhibited infection by almost 100%, as corroborated by infectivity assays and the nucleic acid spot hybridization test. Antiviral activity of MAP extracts was observed against mechanically transmitted viruses but not against aphid-transmitted viruses. Purified MAP showed the same antiviral effect as the crude extracts.

scholarly journals Depurination of plant ribosomes by pokeweed antiviral protein

FEBS Letters ◽  
1990 ◽  
Vol 273 (1-2) ◽  
pp. 144-146 ◽  
Author(s):  
Blair E. Taylor ◽  
James D. Irvin
Keyword(s):  
Pokeweed Antiviral Protein ◽  
Antiviral Protein ◽  
Plant Ribosomes

scholarly journals Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice

2021 ◽  
Vol 22 (3) ◽  
pp. 1434
Author(s):  
Pieter Wytynck ◽  
Jeroen Lambin ◽  
Simin Chen ◽  
Sinem Demirel Asci ◽  
Isabel Verbeke ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Protein Translation ◽  
Ribosome Inactivating Proteins ◽  
Close Proximity ◽  
Inhibit Protein Translation ◽  
Plant Ribosomes ◽  
A Cell ◽  
Type 3

Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.

scholarly journals Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Joel M. J. Tan ◽  
Monica E. Garner ◽  
James M. Regeimbal ◽  
Catherine J. Greene ◽  
Jorge D. Rojas Márquez ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Molecular Mechanisms ◽  
Immune Cell ◽  
Transmembrane Protein ◽  
Foodborne Pathogen ◽  
Systemic Infection ◽  
Cytokine Signaling ◽  
Type I ◽  
Type I Ifn ◽  
Antiviral Protein

AbstractThe type I interferon (IFN) signaling pathway has important functions in resistance to viral infection, with the downstream induction of interferon stimulated genes (ISG) protecting the host from virus entry, replication and spread. Listeria monocytogenes (Lm), a facultative intracellular foodborne pathogen, can exploit the type I IFN response as part of their pathogenic strategy, but the molecular mechanisms involved remain unclear. Here we show that type I IFN suppresses the antibacterial activity of phagocytes to promote systemic Lm infection. Mechanistically, type I IFN suppresses phagosome maturation and proteolysis of Lm virulence factors ActA and LLO, thereby promoting phagosome escape and cell-to-cell spread; the antiviral protein, IFN-induced transmembrane protein 3 (IFITM3), is required for this type I IFN-mediated alteration. Ifitm3−/− mice are resistant to systemic infection by Lm, displaying decreased bacterial spread in tissues, and increased immune cell recruitment and pro-inflammatory cytokine signaling. Together, our findings show how an antiviral mechanism in phagocytes can be exploited by bacterial pathogens, and implicate IFITM3 as a potential antimicrobial therapeutic target.

scholarly journals Isolation and characterization of a novel class of plant antimicrobial peptides form Mirabilis jalapa L. seeds.

1992 ◽  
Vol 267 (4) ◽  
pp. 2228-2233 ◽  
Author(s):  
B P Cammue ◽  
M F De Bolle ◽  
F R Terras ◽  
P Proost ◽  
J Van Damme ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Isolation And Characterization ◽  
Mirabilis Jalapa
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