Improvement of seed yield in Vicia faba L. By using experimental mutagenesis. II. Comparison of gamma-radiation and ethyl-methane-sulphonate (EMS) in production of morphological mutants

Euphytica ◽  
1986 ◽  
Vol 35 (1) ◽  
pp. 49-59 ◽  
Author(s):  
A. Filippetti ◽  
C. De Pace
2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam

2015 ◽  
Vol 35 ◽  
pp. 50-58 ◽  
Author(s):  
P. Gopinath ◽  
P. Pavadai

Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.


2014 ◽  
Vol 22 ◽  
pp. 33-43 ◽  
Author(s):  
S. Gnanamurthy ◽  
D. Dhanavel

Effect of EMS (ethyl methane sulphonate) on induced morphological mutants and chromosomal variation in cowpea was studied using five different doses of mutagen along with a control in randomized blocked design with three replications. The morphological mutants there are two types of viable and chlorophyll mutants. Viable mutant contains tall, dwarf, early maturity, late maturity, leaf mutants pod mutant and flower mutants. The frequency of chlorophyll mutant contains albino, xantha and viridis. This concentration can damage or modify important components of plant cells and have been reported to affect the morphology, anatomy, biochemistry and physiology of plants differentially depending on the concentration level. These effects include changes in the cellular structure and metabolism of the plants e.g., dilation of thylakoid membranes, alteration in photosynthesis, modulation of the antioxidative system and accumulation of phenolic compounds. The morphological and chromosomal variation was found to be mutagen sensitive in somatic cells of cowpea. It was found to increase with increasing the concentration of EMS in Cowpea plants. The chemical mutagen like ethyl methane sulphonate induces high frequency of chromosomal changes like anaphasic bridge; anaphasic laggard, anaphasic bridge and clumbing of chromosome were including control plants also observed.


Author(s):  
K. Geetha ◽  
S. Divya ◽  
S. Srividya

Background: Induction of mutation plays an important role in the breeding programme among modern methods of plant breeding. Genetic variability is prerequisite for crop improvement and through induced mutation it was found to be very effective for creating variability in the quantitative and qualitative characters. Hence, the present study was aimed to induce genetic variability within short time. In the present investigation positive as well as negative impact on quantitative traits were recorded. Methods: The experiment material comprised of seeds of horsegram [Macrotyloma uniflorum (Lam.) Verdc] variety Paiyur 2 which was subjected to ethyl methane sulphonate (02, 0.3, 0.4 and 0.5%), gamma radiation (100, 200, 300 and 400 Gy) and combination treatments. The mutations affecting gross morphological changes in growth and yield characters such as plant habit, flowering, pod morphology, maturity and seed yield were scored as quantitative characters. The micro mutations at population level can be easily detected in the form of increased variations for quantitative traits in the segregation of mutagen treated populations. Micro mutations can alter morpho-physiological characters hence they are of a particular interest to the plant breeders. ANOVA test was performed to determine the significant differences and duncan’s multiple range test (p=0.05) to compare the differences among treatment means. Result: Among the twenty-five treatments studied both the mutagens, gamma radiations and Ethyl Methane Sulphonate proved to be very effective to induce variability in quantitative traits in M2 and M3 generations. The results of Duncan’s multiple range test analysis revealed that the treatment of mutagens in combinations induced more positive effects as compared to that of using alone. The combined treatments were found positive for improvement of primary branches per plant, no. of pods per plant, pod length, no. of seeds/pod and seed yield per plant in M2 and M3 generations whereas the treatment of gamma irradiation imparted its significant role for improvement of plant height, days required for 50% flowering, days to maturity and 1000 seed weight in both M2 and M3 generations whereas the treatment with Ethyl Methane Sulphonate is useful for improving no. of pods per plant and no. of seeds/pod in M2 generation alone.


Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1088
Author(s):  
Mohamed Houssemeddine Sellami ◽  
Antonella Lavini ◽  
Davide Calandrelli ◽  
Giuseppe De Mastro ◽  
Cataldo Pulvento

Faba beans (Vicia faba L.), also known as fava beans, like other crops, are influenced by several factors: their genotype, environment, and management, as well as the interaction between these, have an important impact on seed yielding and seed quality traits. This study was conducted at three locations in South Italy between 2017 and 2019 to evaluate the sowing date effect on yield and yield components of three Vicia faba L., originating from cool climates. The results showed that seed yield (SY) and yield components declined with sowing delay. The crop’s environment (year × site) and management (sowing date) were found to explain 34.01% and 42.95% of the total seed yield variation, respectively. The data showed that the tested genotypes were positively influenced by the environment with sandy loam soil and early winter sowing date, resulting in either a greater number of SY and THS than in the other environment. The three faba bean genotypes showed tolerance to winter frost conditions in the two growing seasons.


1986 ◽  
Vol 66 (2) ◽  
pp. 235-239 ◽  
Author(s):  
G. DUC ◽  
G. G. ROWLAND ◽  
J. PICARD

A near-isogenic faba bean (Vicia faba L.) line, segregating in a 1:1 ratio of male-fertile to male-sterile plants, was used to study the importance of insect pollinators on ovule fertilization and yield components of the first five flowering nodes. The fertilization of ovules from open-pollinated plants at the higher insect pollinator site of Dijon, France was 83%, as compared with 50% at the lower insect pollinator activity site of Saskatoon, Canada. No significant differences in total fertilized ovules were found between male-fertile and male-sterile plants at Dijon, but male-fertile plants at Saskatoon had significantly more fertilized ovules than male-sterile plants. Seed production on the first five flowering nodes was significantly greater at Saskatoon. Thus, while insect pollinators are important in determining the potential yield of a faba bean plant, other factors, such as environment, determine the ultimate yield.Key words: Vicia faba, ovule fertilization, seed yield, faba bean, male sterility


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