Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts

1993 ◽  
Vol 21 (5) ◽  
pp. 871-884 ◽  
Author(s):  
Keerti S. Rathore ◽  
Vijay K. Chowdhury ◽  
Thomas K. Hodges
Keyword(s):  
Selectable Marker ◽  
Marker Gene ◽  
Selectable Marker Gene ◽  
Herbicide Resistant ◽  
Rice Plants

scholarly journals Targeted Transgene Expression in Rice Using a Callus Strong Promoter for Selectable Marker Gene Control

2020 ◽  
Vol 11 ◽  
Author(s):  
Jie Zhou ◽  
Dongyue Li ◽  
Chao Zheng ◽  
Rumeng Xu ◽  
Ersong Zheng ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Selectable Marker ◽  
Marker Gene ◽  
Camv 35S Promoter ◽  
35S Promoter ◽  
Specific Marker ◽  
Selectable Marker Gene ◽  
Strong Promoter ◽  
Camv 35S ◽  
Rice Plants

Precise expression of a transgene in the desired manner is important for plant genetic engineering and gene function deciphering, but it is a challenge to obtain specific transgene expression free from the interference of the constitutive promoters used to express the selectable marker gene, such as the Cauliflower mosaic virus (CaMV) 35S promoter. So, the solutions to avoid these inappropriate regulations are largely demanded. In this study, we report the characterization of a callus strong promoter (CSP1) in rice and its application for accurate transgene expression. Our results indicate that the high expression of the CSP1 promoter in the callus enables efficient selection of hygromycin equivalent to that provided by the CaMV 35S promoter, whereas its expression in other tissues is low. To evaluate possible leaky effects, the expression of a β-glucuronidase reporter driven by six specific promoters involving hormone signaling, pathogen response, cell fate determination, and proliferation was observed in transgenic rice plants generated by CSP1-mediated selection. Distinct β-glucuronidase expression was found consistently in most of the transgenic lines obtained for each promoter. In addition, we applied these specific marker lines to investigate the root cellular responses to exogenous cytokinin and auxin treatment. The results reveal that the root growth inhibition by cytokinin was differently regulated at high and low concentrations. In summary, we have established the feasibility of using callus-specific promoter-dependent selection to mitigate the transgene misexpression in rice. By enabling efficient transformation, rice plants with reliable transgene expression will be easily acquired for broad applications.

A plant selectable marker gene based on the detoxification of the herbicide dalapon

1992 ◽  
Vol 11 (12) ◽  
Author(s):  
Vicky Buchanan-Wollaston ◽  
Andrea Snape ◽  
Frank Cannon
Keyword(s):  
Selectable Marker ◽  
Marker Gene ◽  
Selectable Marker Gene

scholarly journals Manipulating genome of diploid potato inbred line Solanumchacoense M6 using selectable marker gene

2020 ◽  
Vol 44 (4) ◽  
pp. 399-407
Author(s):  
Sarbesh Das DANGOL ◽  
İlknur YEL ◽  
Mehmet Emin ÇALIŞKAN ◽  
Allah BAKHSH
Keyword(s):  
Inbred Line ◽  
Selectable Marker ◽  
Marker Gene ◽  
Diploid Potato ◽  
Selectable Marker Gene

A murine cell line producing HTLV-I pseudotype virions carrying a selectable marker gene

Virology ◽  
1991 ◽  
Vol 180 (1) ◽  
pp. 420-424 ◽  
Author(s):  
Richard G. Vile ◽  
Thomas F. Schulz ◽  
Olivier F. Danos ◽  
Mary K.L. Collins ◽  
Robin A. Weiss
Keyword(s):  
Cell Line ◽  
Selectable Marker ◽  
Marker Gene ◽  
Selectable Marker Gene ◽  
Murine Cell ◽  
Murine Cell Line

Fidelity of a simple Liberty leaf-painting assay to validate transgenic maize plants expressing the selectable marker gene, bar

2017 ◽  
Vol 31 (4) ◽  
pp. 628-636 ◽  
Author(s):  
K. Rajasekaran ◽  
R. Majumdar ◽  
C. Sickler ◽  
Q. Wei ◽  
J. Cary ◽  
...  
Keyword(s):  
Selectable Marker ◽  
Marker Gene ◽  
Transgenic Maize ◽  
Selectable Marker Gene ◽  
Maize Plants
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