The extracellular matrix (mesoglea) of hydrozoan jellyfish and its ability to support cell adhesion and spreading

Hydrobiologia ◽  
1991 ◽  
Vol 216-217 (1) ◽  
pp. 3-10 ◽  
Author(s):  
V. Schmid ◽  
A. Bally ◽  
K. Beck ◽  
M. Haller ◽  
W. K. Schlage ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Support Cell

Membrane Lipid Composition Plays a Central Role in the Maintenance of Epithelial Cell Adhesion to the Extracellular Matrix

Lipids ◽  
2008 ◽  
Vol 43 (4) ◽  
pp. 343-352 ◽  
Author(s):  
María Gabriela Márquez ◽  
Francisco Leocata Nieto ◽  
María C. Fernández-Tome ◽  
Nicolás Octavio Favale ◽  
Norma Sterin-Speziale
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Membrane Lipid Composition

scholarly journals Persistence of intramyocardially transplanted murine induced pluripotent stem cell-derived cardiomyocytes from different developmental stages

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gabriel Peinkofer ◽  
Martina Maass ◽  
Kurt Pfannkuche ◽  
Agapios Sachinidis ◽  
Stephan Baldus ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Developmental Stage ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

Abstract Background Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) are regarded as promising cell type for cardiac cell replacement therapy, but it is not known whether the developmental stage influences their persistence and functional integration in the host tissue, which are crucial for a long-term therapeutic benefit. To investigate this, we first tested the cell adhesion capability of murine iPSC-CM in vitro at three different time points during the differentiation process and then examined cell persistence and quality of electrical integration in the infarcted myocardium in vivo. Methods To test cell adhesion capabilities in vitro, iPSC-CM were seeded on fibronectin-coated cell culture dishes and decellularized ventricular extracellular matrix (ECM) scaffolds. After fixed periods of time, stably attached cells were quantified. For in vivo experiments, murine iPSC-CM expressing enhanced green fluorescent protein was injected into infarcted hearts of adult mice. After 6–7 days, viable ventricular tissue slices were prepared to enable action potential (AP) recordings in transplanted iPSC-CM and surrounding host cardiomyocytes. Afterwards, slices were lysed, and genomic DNA was prepared, which was then used for quantitative real-time PCR to evaluate grafted iPSC-CM count. Results The in vitro results indicated differences in cell adhesion capabilities between day 14, day 16, and day 18 iPSC-CM with day 14 iPSC-CM showing the largest number of attached cells on ECM scaffolds. After intramyocardial injection, day 14 iPSC-CM showed a significant higher cell count compared to day 16 iPSC-CM. AP measurements revealed no significant difference in the quality of electrical integration and only minor differences in AP properties between d14 and d16 iPSC-CM. Conclusion The results of the present study demonstrate that the developmental stage at the time of transplantation is crucial for the persistence of transplanted iPSC-CM. iPSC-CM at day 14 of differentiation showed the highest persistence after transplantation in vivo, which may be explained by a higher capability to adhere to the extracellular matrix.

scholarly journals Mast Cell Adhesion to Extracellular Matrix: Local Effects of Acute Phase Reactants

1997 ◽  
Vol 113 (1-3) ◽  
pp. 295-296 ◽  
Author(s):  
Rami Hershkoviz ◽  
Liana Preciado-Patt ◽  
Ofer Lider ◽  
Mati Fridkin ◽  
Yoseph A. Mekori
Keyword(s):  
Extracellular Matrix ◽  
Mast Cell ◽  
Cell Adhesion ◽  
Acute Phase ◽  
Local Effects ◽  
Acute Phase Reactants
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