Genetic control of somatic embryogenesis in alfalfa (Medicago sativa L. cv. Adriana)

Euphytica ◽  
1995 ◽  
Vol 81 (2) ◽  
pp. 151-155 ◽  
Author(s):  
F. Crea ◽  
M. Bellucci ◽  
F. Damiani ◽  
S. Arcioni
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Genetic Control ◽  
Medicago Sativa L

Genetic control of somatic embryogenesis in alfalfa

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 474-477 ◽  
Author(s):  
G. A. Kielly ◽  
S. R. Bowley
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Genetic Control ◽  
In Vitro Regeneration ◽  
Breeding Population ◽  
Medicago Sativa L

The genetic control of somatic embryogenesis in alfalfa (Medicago sativa L.) was studied using one nonembryogenic and three embryogenic genotypes: A70-34, a selection from 'Rangelander'; RA3, a selection from 'Regen-S'; and C2-4, a selection from a breeding population that had A70-34 in its pedigree. Crosses of embryogenic × embryogenic and embryogenic × nonembryogenic and S1 and BC1 testcrosses were evaluated for in vitro regeneration. Selfing reduced the expression of the trait. Somatic embryogenesis was dominant and explained by two loci. All three regenerating genotypes shared a common genetic system.Key words: alfalfa, Medicago sativa, somatic embryogenesis, tissue culture.

Field evaluation following two cycles of backcross transfer of somatic embryogenesis to commercial alfalfa germplasm

1993 ◽  
Vol 73 (1) ◽  
pp. 131-137 ◽  
Author(s):  
S. R. Bowley ◽  
G. A. Kielly ◽  
K. Anandarajah ◽  
B. D. McKersie ◽  
T. Senaratna
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Seed Yield ◽  
Field Experiments ◽  
Field Evaluation ◽  
Growth Characteristics ◽  
Seed Technology ◽  
Artificial Seed ◽  
Medicago Sativa L

For successful application of artificial seed technology to alfalfa (Medicago sativa L.), parental plants must possess the necessary genes for somatic embryogenesis and produce progeny having high commercial value. A backcross procedure was initiated to transfer the ability to form somatic embryos from genotype A70-34, a selection from the cultivar Rangelander, to multiple-pest-resistant alfalfa germplasm. The objectives of this study were to evaluate the growth characteristics and seed yield of the F1; BC1 and BC2 generations to determine if introgression with commercial germplasm had improved the agronomic features of the embryogenic germplasm. This study consisted of two field experiments, each conducted at two locations. One experiment evaluated herbage growth characteristics and herbage production at Elora and Woodstock, Ontario, and the other evaluated seed yield at Elora and Delhi, Ontario. Significant increases in fall regrowth height, leaflet length:width ratio, and seed yield were detected over the cycles of crossing. By the BC2, fall dormancy and leaflet length:width ratio were similar to those in commercial populations. Although improvements in seed yield were detected, the BC2 was inferior in seed yield compared with commercial germplasm, and further introgression and (or) conscious selection for improved seed yield will be required. Through a population backcross procedure, it appears possible to develop commercial alfalfa germplasm capable of in vitro manipulation. Key words: Alfalfa, Medicago sativa L., somatic embryogenesis, artificial seed, backcross

Alfalfa (Medicago sativa) Somatic Embryogenesis: Genetic Control and Introduction of Favourable Alleles into Elite Argentinean Germplasm

2004 ◽  
Vol 77 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Roxana Moltrasio ◽  
Claudio G. Robredo ◽  
María Cristina Gómez ◽  
Antonio H. Díaz Paleo ◽  
Daniel G. Díaz ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Genetic Control

Changes in jasmonates and 12-oxophytodienoic acid contents of Medicago sativa L. during somatic embryogenesis

2005 ◽  
Vol 27 (4) ◽  
pp. 497-504 ◽  
Author(s):  
Izabela Rudu ◽  
Ewa K. pczy ska ◽  
Jan K pczy ski ◽  
Claus Wasternack ◽  
Otto Miersch
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Medicago Sativa L

A transplant plug technique for production of alfalfa (Medicago sativa L.) plants from somatic embryos

1992 ◽  
Vol 72 (2) ◽  
pp. 483-485 ◽  
Author(s):  
A. R. McElroy ◽  
D. C. W. Brown
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Somatic Embryos ◽  
Potting Media ◽  
Medicago Sativa L

A transplant plug technique was developed that uses in vitro somatic embryogenesis techniques to mass-multiply alfalfa plants in a form suitable for direct transplanting. The plug contains potting media covered with an agar nutrient cap. Plants develop from embryos placed on the cap and then establish in the potting media.Key words: Hybrid alfalfa, Medicago sativa L., tissue culture, transplant plug, somatic embryogenesis

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