Influence of exogenous abscisic acid on germination and plantlet conversion frequencies of hybrid larch somatic embryos (Larix � leptoeuropaea)

1994 ◽  
Vol 15 (2) ◽  
pp. 175-182 ◽  
Author(s):  
Philippe Label ◽  
Marie-Anne Lelu
2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.


1997 ◽  
Vol 40 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Soh Woong Young ◽  
Duck Yee Cho ◽  
Kyung Sik Kim ◽  
Byung Yun Sun

Plant Science ◽  
1997 ◽  
Vol 124 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Laurent Linossier ◽  
Philippe Veisseire ◽  
Françoise Cailloux ◽  
Alain Coudret

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