A plant cell bioreactor with medium-perfusion for control of somatic embryogenesis in liquid cell suspensions

1996 ◽  
Vol 20 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Simon D. Moorhouse ◽  
Graham Wilson ◽  
Michael J. Hennerty ◽  
Christopher Selby ◽  
Se�n Mac An tSaoir
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Cell ◽  
Cell Suspensions ◽  
Liquid Cell

scholarly journals MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

2018 ◽  
Vol 6 ◽  
pp. 1185-1191
Author(s):  
Minh Van Tran
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Cell ◽  
Embryonic Cell ◽  
Cell Suspensions ◽  
Ms Medium ◽  
Callus Initiation ◽  
Set Up ◽  
Planting Materials ◽  
In Vitro Embryogenesis

Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

Plant cell suspensions resistant to and recalcitrant to cryopreservation procedures

Cryobiology ◽  
1988 ◽  
Vol 25 (6) ◽  
pp. 534
Author(s):  
F. van Iren ◽  
J. Schripsema ◽  
E.W.M. Schrijne-Makers ◽  
R. van der Heijden ◽  
R. Verpoorte ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Suspensions

Estimation of Growth Yield and Maintenance Coefficient of Plant Cell Suspensions

1991 ◽  
Vol 38 (10) ◽  
pp. 1131-1136 ◽  
Author(s):  
Sherry Rae Schnapp ◽  
Wayne R. Curtis ◽  
Ray A. Bressan ◽  
Paul M. Hasegawa
Keyword(s):  
Plant Cell ◽  
Growth Yield ◽  
Cell Suspensions ◽  
Maintenance Coefficient

Somatic embryogenesis and plant regeneration through cell suspensions inMusa acuminata

1993 ◽  
Vol 29 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Claudia G. Marroquin ◽  
Cécile Paduscheck ◽  
Jean V. Escalant ◽  
Claude Teisson
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspensions

Rheological properties of plant cell suspensions

1992 ◽  
Vol 8 (5) ◽  
pp. 413-420 ◽  
Author(s):  
Rabia Ballica ◽  
Dewey D. Y. Ryu ◽  
Robert L. Powell ◽  
Derek Owen
Keyword(s):  
Rheological Properties ◽  
Plant Cell ◽  
Cell Suspensions

Phytostat for Continuous Culture and Automatic Sampling of Plant-Cell Suspensions

Science ◽  
1968 ◽  
Vol 159 (3814) ◽  
pp. 540-542 ◽  
Author(s):  
R. A. Miller ◽  
J. P. Shyluk ◽  
O. L. Gamborg ◽  
J. W. Kirkpatrick
Keyword(s):  
Continuous Culture ◽  
Plant Cell ◽  
Cell Suspensions ◽  
Automatic Sampling
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