Bolivia potato germplasm collecting expeditions 1993, 1994: Taxonomy and new germplasm resources

Euphytica ◽  
1994 ◽  
Vol 79 (1-2) ◽  
pp. 137-148 ◽  
Author(s):  
David M. Spooner ◽  
Ronald G. van den Berg ◽  
Willman García ◽  
María Luisa Ugarte
Keyword(s):  
Germplasm Resources ◽  
Potato Germplasm ◽  
Germplasm Collecting ◽  
New Germplasm

Potato germplasm collecting expedition to Mexico in 1997: Taxonomy and new germplasm resources

2000 ◽  
Vol 77 (4) ◽  
pp. 261-270 ◽  
Author(s):  
David M. Spooner ◽  
Antonio Rivera-Peña ◽  
Ronald G. van den Berg ◽  
Konrad Schüler
Keyword(s):  
Germplasm Resources ◽  
Potato Germplasm ◽  
Collecting Expedition ◽  
Germplasm Collecting ◽  
New Germplasm

Ecuador, 1991 potato germplasm collecting expedition: taxonomy and new germplasm resources

Euphytica ◽  
1992 ◽  
Vol 60 (3) ◽  
pp. 159-169
Author(s):  
David M. Spooner ◽  
Raúl T. Castillo ◽  
Luis J. López
Keyword(s):  
Germplasm Resources ◽  
Potato Germplasm ◽  
Collecting Expedition ◽  
Germplasm Collecting ◽  
New Germplasm

Colombia and Venezuela 1992 wild potato (Solanum sect. Petota) germplasm collecting expedition: taxonomy and new germplasm resources

Euphytica ◽  
1995 ◽  
Vol 81 (1) ◽  
pp. 45-56 ◽  
Author(s):  
David M. Spooner ◽  
Ra�l Castillo T. ◽  
Luis L�pez J. ◽  
Ram�n Pineda ◽  
Ra�l Le�n P. ◽  
...  
Keyword(s):  
Wild Potato ◽  
Germplasm Resources ◽  
Collecting Expedition ◽  
Germplasm Collecting ◽  
Solanum Sect ◽  
New Germplasm

Potato germplasm collecting expedition to the Guaitecas and chonos Archipelagos, Chile, 1990

1993 ◽  
Vol 36 (4) ◽  
pp. 309-316 ◽  
Author(s):  
Andres Contreras ◽  
Luigi Ciampi ◽  
Stefano Padulosi ◽  
David M. Spooner
Keyword(s):  
Potato Germplasm ◽  
Collecting Expedition ◽  
Germplasm Collecting

Mexico, 1988 potato germplasm collecting expedition and utility of the Mexican potato species

1991 ◽  
Vol 68 (1) ◽  
pp. 29-43 ◽  
Author(s):  
David M. Spooner ◽  
John Bamberg ◽  
J. Peter Hjerting ◽  
José Gómez
Keyword(s):  
Potato Species ◽  
Potato Germplasm ◽  
Collecting Expedition ◽  
Germplasm Collecting

scholarly journals Genome-Wide Association Analysis of Late Blight Resistance Traits in Potato Germplasm Resources

2020 ◽  
Author(s):  
Fang Wang ◽  
Meiling Zou ◽  
Long Zhao ◽  
Huaqing Li ◽  
Zhiqiang Xia ◽  
...  
Keyword(s):  
Late Blight ◽  
Candidate Genes ◽  
Association Analysis ◽  
Late Blight Resistance ◽  
Genome Wide Association ◽  
Blight Resistance ◽  
Germplasm Resources ◽  
Potato Germplasm ◽  
Genome Wide ◽  
Population Structure Analysis

Abstract Background: Potatoes are dicotyledonous plants of the genus Solanum, family Solanaceae, and contain large amounts of starch, proteins, and trace elements required by the human. Potato late blight is the main disease hindering potato production. In this study, Phytophthora infestans were used to quantify late blight resistance in 284 germplasm resources, and resistance genes were mined through genome-wide association analysis.Results: The results showed that among the 284 potato germplasm resources, 37 showed immunity, 15 were highly resistant to late blight, 30 were moderately resistant to late blight, 107 were moderately susceptible to late blight, and 95 were highly susceptible to late blight. Through screening and filtering, 22,489 high-quality single-nucleotide polymorphisms (SNPs) and indels were obtained. Through population structure analysis and principal-component analysis, 284 germplasm resources were divided into eight subgroups, which was consistent with the results of the phylogenetic tree analysis. The genetic diversity index of the 284 potato germplasm resources was 0.2161, and the differentiation index of each subgroup was 0.0251-0.1489. A mixed linear model was built to perform an association analysis on the diameter of the lesions identified from isolated leaves of potato affected by late blight. The genes within 100 kb of both sides of the obtained significant SNP loci were searched and functionally annotated, and 18 candidate genes were obtained. Twenty-two candidate genes were obtained from the association analysis of disease resistance grade.Conclusions: 284 potato germplasm resources were used to identify for Phytophthora infestans resistance. The potato germplasm resources were divided into 8 subgroups by population structure analysis, and the main differentiation among subgroups was moderate. Candidate genes were mined by genome-wide association analysis.The results of this study provides the foundation for the genetic improvement of potato varieties resistant to late blight.

scholarly journals CRISPR-Cas9 Gene Editing for Fruit and Vegetable Crops: Strategies and Prospects

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 193
Author(s):  
Lili Wan ◽  
Zhuanrong Wang ◽  
Mi Tang ◽  
Dengfeng Hong ◽  
Yuhong Sun ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Abiotic Stress Tolerance ◽  
Whole Genome Sequencing Data ◽  
Germplasm Resources ◽  
Vegetable Crops ◽  
Fruit And Vegetable ◽  
Sequencing Data ◽  
Pests And Diseases ◽  
Breeding Technique ◽  
New Germplasm

Fruit and vegetable crops are rich in dietary fibre, vitamins and minerals, which are vital to human health. However, many biotic stressors (such as pests and diseases) and abiotic stressors threaten crop growth, quality, and yield. Traditional breeding strategies for improving crop traits include a series of backcrosses and selection to introduce beneficial traits into fine germplasm, this process is slow and resource-intensive. The new breeding technique known as clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 (Cas9) has the potential to improve many traits rapidly and accurately, such as yield, quality, disease resistance, abiotic stress tolerance, and nutritional aspects in crops. Because of its simple operation and high mutation efficiency, this system has been applied to obtain new germplasm resources via gene-directed mutation. With the availability of whole-genome sequencing data, and information about gene function for important traits, CRISPR-Cas9 editing to precisely mutate key genes can rapidly generate new germplasm resources for the improvement of important agronomic traits. In this review, we explore this technology and its application in fruit and vegetable crops. We address the challenges, existing variants and the associated regulatory framework, and consider future applications.

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