The effect of transfers of alien genes for leaf rust resistance on the agronomic and quality characteristics of wheat

Euphytica ◽  
1989 ◽  
Vol 44 (1-2) ◽  
pp. 65-72 ◽  
Author(s):  
D. R. Knott
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Quality Characteristics ◽  
Alien Genes

AGRONOMIC AND QUALITY CHARACTERISTICS OF WHEAT LINES WITH LEAF RUST RESISTANCE DERIVED FROM TRITICUM SPELTOIDES

1981 ◽  
Vol 23 (3) ◽  
pp. 475-480 ◽  
Author(s):  
D. R. Knott ◽  
J. Dvořák
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Quality Characteristics ◽  
Puccinia Recondita ◽  
Recurrent Parent ◽  
Wheat Lines ◽  
Speltoides Chromosome ◽  
Deleterious Genes

Eleven lines of wheat (Triticum aestivum L.) carrying resistance to leaf rust (Puccinia recondita Rob. ex. Desm.) derived from five accessions of Triticum speltoides Tausch were grown in yield tests in 1977 and 1979. The grain was tested for quality characteristics in both years. Although the lines had been backcrossed four or five times to either Manitou or Neepawa, only four of the eleven showed any real promise of equalling their recurrent parent in agronomic and quality characteristics. Lines derived from the same accession of T. speltoides were surprisingly variable. The generally deleterious effects of the transferred chromatin are due either to genes linked to the genes for leaf rust resistance plus incomplete compensation by the speltoides chromosome segment for the aestivum segment it replaced, or to the effects of additional translocations that were not eliminated during backcrossing. A second cycle of homoeologous recombination is proposed as a way to eliminate some of the deleterious genes.

Lr80: A new and widely effective source of leaf rust resistance of wheat for enhancing diversity of resistance among modern cultivars

2021 ◽  
Author(s):  
Subodh Kumar ◽  
Subhash C. Bhardwaj ◽  
Om P. Gangwar ◽  
Akanksha Sharma ◽  
Naeela Qureshi ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Effective Source

scholarly journals Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Agronomic Traits ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.

scholarly journals Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR

2021 ◽  
Vol 16 (1) ◽  
pp. 172-183
Author(s):  
Agnieszka Tomkowiak ◽  
Roksana Skowrońska ◽  
Michał Kwiatek ◽  
Julia Spychała ◽  
Dorota Weigt ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Leaf Rust ◽  
Common Wheat ◽  
Fungal Pathogens ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Field Conditions ◽  
Pcr Marker ◽  
Specific Sequence ◽  
Cereal Species

Abstract Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes Lr34 and Lr46. This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker csLV was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene Lr34, gene Lr46 was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker wmc44 of gene Lr46 was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.

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