Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. I. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase

1993 ◽  
Vol 23 (4) ◽  
pp. 697-706 ◽  
Author(s):  
J�rn G�rlach ◽  
Andreas Beck ◽  
John M. Henstrand ◽  
Avtar K. Handa ◽  
Klaus M. Herrmann ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Differential Expression ◽  
Shikimate Pathway ◽  
Genes Encoding ◽  
Phosphate Synthase

scholarly journals Differential expression of 5-enol-pyruvyl-shikimate-3-phosphate synthase isoforms in elicitor-treated, cultured maize cells

2002 ◽  
Vol 29 (12) ◽  
pp. 1483 ◽  
Author(s):  
Giuseppe Forlani
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Zea Mays L ◽  
Cultured Cells ◽  
Amino Acid Content ◽  
Shikimate Pathway ◽  
Activity Levels ◽  
Sharp Rise ◽  
Enzyme Form ◽  
Genes Encoding ◽  
Phosphate Synthase

The expression of two 5-enol-pyruvyl-shikimate-3-phosphate synthase (EC 2.5.1.19) isoforms was investigated in Zea mays L. suspension-cultured cells following exposure to a fungal elicitor. Activity levels of isozyme II specifically increased soon after treatment, in strict connection with induction of phenylalanine ammonia-lyase (PAL) and attainment of a new free-phenylalanine homeostasis at a higher concentration. However, a few days later, activity of the other enzyme form was also significantly enhanced, concomitant with a sharp rise in overall amino acid content, a further increase in PAL level and a resumption of cell lysis. Besides strengthening the hypothesis that an entire set of genes encoding for shikimate pathway enzymes (whose expression is specifically involved in plant dynamic defence) may exist, a general change in the levels of several amino acids seems to point towards a reprogramming of their metabolism in elicited cells.

scholarly journals Transcription Profiles Analysis of Genes Encoding 1-Deoxy-D-xylulose 5-Phosphate Synthase and 2C-Methyl-D-erythritol 4-Phosphate Synthase in Plaunotol Biosynthesis from Croton stellatopilosus

2008 ◽  
Vol 31 (5) ◽  
pp. 852-856 ◽  
Author(s):  
Juraithip Wungsintaweekul ◽  
Tanawan Sirisuntipong ◽  
Damrong Kongduang ◽  
Thanaisawan Losuphanporn ◽  
Anan Ounaroon ◽  
...  
Keyword(s):  
Transcription Profiles ◽  
Genes Encoding ◽  
Phosphate Synthase

Screening for trans-acting factors and other factors involved in the activating or silencing of the γ-globin gene during human ontogeny

2007 ◽  
Vol 85 (3) ◽  
pp. 347-357
Author(s):  
Yan-Ni Ma ◽  
Xin Zhang ◽  
Jun-Wu Zhang ◽  
Xin-Hua Zhang ◽  
Rong-Xin Wang
Keyword(s):  
Bone Marrow ◽  
Differential Expression ◽  
Globin Gene ◽  
Fetal Hemoglobin ◽  
Pcr Analysis ◽  
Reverse Transcription Pcr ◽  
Genes Encoding ◽  
Hereditary Persistence ◽  
Differential Display Reverse Transcription ◽  
Human Ontogeny

Researchers hope to increase γ-globin expression by controlling potential trans-acting factors that specifically activate the γ-globin gene in fetuses or silence this gene in adults to potentially treat sickle cell disease and β-thalassemias. To characterize genes encoding such factors, we analyzed the differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal adult bone marrow, umbilical cord blood, and bone marrow from a patient with heterocellular hereditary persistence of fetal hemoglobin. Using differential-display – reverse-transcription PCR analysis, we identified a number of genes with differential expression in the above-mentioned cells. The differential expression of some genes was also confirmed by quantitative real-time PCR. Our data provide important clues for identifying and validating trans-activators that activate the γ-globin gene in fetuses, and trans-acting factors involved in silencing the γ-globin gene in adults.

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