Functional analysis of a recently originating, atypical presequence: mitochondrial import and processing of GUS fusion proteins in transgenic tobacco and yeast

1995 ◽  
Vol 29 (3) ◽  
pp. 519-533 ◽  
Author(s):  
Jintai Huang ◽  
Charles S. Levings
2005 ◽  
Vol 15 (4) ◽  
pp. 361-364 ◽  
Author(s):  
Ka Leung Fung ◽  
Yin Fun Yim ◽  
Yu Chung Tse ◽  
Yansong Miao ◽  
Samuel S.M. Sun ◽  
...  

Seeds that store proteins in protein storage vacuoles are attractive bioreactors for producing and storing large amounts of pharmaceutical proteins. However, foreign proteins expressed in transgenic plants are subjected to the delivery and modification processes present within plant cells. Here, it is demonstrated that unique membrane sequences deliver a yellow fluorescent protein (YFP) to the seed protein storage vacuoles in transgenic tobacco (Nicotiana tabacum L.) plants, where the YFP is then separated from its membrane anchors. This precise targeting and separation is required for the successful delivery of useful proteins to seed protein storage vacuoles for their stable accumulation in transgenic crops.


2007 ◽  
Vol 58 (8) ◽  
pp. 2053-2067 ◽  
Author(s):  
Li Ding ◽  
Daniel Hofius ◽  
Mohammad-Reza Hajirezaei ◽  
Alisdair R. Fernie ◽  
Frederik Börnke ◽  
...  

2010 ◽  
Vol 38 (5) ◽  
pp. 3285-3291 ◽  
Author(s):  
Miao-yun Xu ◽  
Xi Zhang ◽  
Lan Zhang ◽  
Yan-zhong Luo ◽  
Yun-liu Fan ◽  
...  

2019 ◽  
Vol 61 (9) ◽  
pp. 703-713 ◽  
Author(s):  
Chunling Zhang ◽  
Yaqin Wang ◽  
Wenjing Wang ◽  
Zhe Cao ◽  
Qiang Fu ◽  
...  

1989 ◽  
Vol 1 (6) ◽  
pp. 599 ◽  
Author(s):  
Karen E. Broglie ◽  
Phyllis Biddle ◽  
Robert Cressman ◽  
Richard Broglie

2014 ◽  
Vol 42 (8) ◽  
pp. S60
Author(s):  
Anna Skucha ◽  
Alexey Stukalov ◽  
Matthias Muhar ◽  
Jacques Colinge ◽  
Keiryn Bennett ◽  
...  

2020 ◽  
Vol 69 (4) ◽  
pp. 756-764 ◽  
Author(s):  
Fatemeh Khodadadi ◽  
Masoud Tohidfar ◽  
Kourosh Vahdati ◽  
Abhaya M. Dandekar ◽  
Charles A. Leslie

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