Induction of nodule primordia on Phaseolus and Acacia by lipo-chitin oligosaccharide nodulation signals from broad-host-range Rhizobium strain GRH2

1995 ◽  
Vol 29 (3) ◽  
pp. 465-477 ◽  
Author(s):  
Isabel M. López-Lara ◽  
Koen M. G. M. van Der Drift ◽  
Anton A. N. van Brussel ◽  
Johan Haverkamp ◽  
Ben J. J. Lugtenberg ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Rhizobium Strain

Nitrogenase Genes in the Fast-Growing Broad-Host Range Rhizobium Strain ANU240

1984 ◽  
pp. 671-671 ◽  
Author(s):  
J. Badenoch-Jones ◽  
C. Bates ◽  
K. Scott ◽  
B. Rolfe ◽  
J. Shine
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Rhizobium Strain ◽  
Fast Growing ◽  
Nitrogenase Genes

Structural and functional analysis of nitrogenase genes from the broad-host-range Rhizobium strain ANU240

Gene ◽  
1989 ◽  
Vol 77 (1) ◽  
pp. 141-153 ◽  
Author(s):  
J. Badenoch-Jones ◽  
T.A. Holton ◽  
C.M. Morrison ◽  
K.F. Scott ◽  
J. Shine
Keyword(s):  
Functional Analysis ◽  
Host Range ◽  
Broad Host Range ◽  
Rhizobium Strain ◽  
Nitrogenase Genes

scholarly journals 1615. Isolation of Lytic Bacteriophages with Broad Host Range Activity Against Pseudomonas aeruginosa Strains Isolated from Respiratory Samples from Cystic Fibrosis Patients Intended for Therapeutic Application

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S801-S801
Author(s):  
Jose Alexander ◽  
Daniel Navas ◽  
Marly Flowers ◽  
Angela Charles ◽  
Amy Carr
Keyword(s):  
Cystic Fibrosis ◽  
Host Range ◽  
Phage Therapy ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Plaque Morphology ◽  
Broad Host Range ◽  
Chronic Infections ◽  
Clinically Significant ◽  
Host Target

Abstract Background With the rise of the antimicrobial resistance between different genera and species of bacteria, Phage Therapy is becoming a more realistic and accessible option for patients with limited or no antimicrobial options. Being able to have rapid access to a collection of clinical active phages is key for rapid implementation of phage therapy. The Microbiology Department at AdventHealth Orlando is performing routine screening of environmental and patient samples for isolation of phages against non-fermenting Gram negative bacteria to develop a Phage Bank. Methods Protocols for phage isolation from environmental sources such as lakes, rivers and sewers and clinical samples were developed. A series of respiratory, throat, stool and urine samples were processed following an internal protocol that includes centrifugation, filtration and enrichment. Clinical samples were centrifugated for 10 minutes, filtered using 0.45µm centrifugation filters, seeded with targeted host bacteria (clinical isolates) and incubated at 35°C for 24 hours. The enriched samples were centrifugated and filtered for a final phage enriched solution. Screening and isolation were performed using the Gracia method over trypticase soybean agar (TSA) for plaque morphology and quantification. Host range screening of other clinical isolates of P. aeruginosa was performed using the new isolated and purified phages. Results 4 lytic phages against clinical strains of P. aeruginosa from patient with diagnosis of cystic fibrosis (CF), were isolated and purified from 4 different respiratory samples, including sputum and bronchial alveolar lavage. All phages showed phenotypical characteristics of lytic activity. 1 phage was active against 4 strains of P. aeruginosa, 1 phage was active against 2 strains of P. aeruginosa and the remaining 2 phages were active only against the initial host target strain. Conclusion With this study we demonstrated the potential use of clinical samples as source for isolating active bacteriophages against clinically significant bacteria strains. Clinical samples from vulnerable population of patients with chronic infections are part of our routine “phage-hunting” process to stock and grow our Phage Bank project for future clinical use. Disclosures All Authors: No reported disclosures

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