Photosystem II characteristics of a constructedSynechocystis 6803 mutant lacking synthesis of the D1 polypeptide

1990 ◽  
Vol 14 (6) ◽  
pp. 1051-1054 ◽  
Author(s):  
Fredrik Nilsson ◽  
Bertil Andersson ◽  
Christer Jansson
Keyword(s):  
Photosystem Ii ◽  
D1 Polypeptide

Histidine 332 of the D1 Polypeptide Modulates the Magnetic and Redox Properties of the Manganese Cluster and Tyrosine YZin Photosystem II†

Biochemistry ◽  
2000 ◽  
Vol 39 (2) ◽  
pp. 470-478 ◽  
Author(s):  
Richard J. Debus ◽  
Kristy A. Campbell ◽  
Jeffrey M. Peloquin ◽  
Donna P. Pham ◽  
R. David Britt
Keyword(s):  
Photosystem Ii ◽  
Redox Properties ◽  
Manganese Cluster ◽  
D1 Polypeptide

Glutamate 189 of the D1 Polypeptide Modulates the Magnetic and Redox Properties of the Manganese Cluster and Tyrosine YZin Photosystem II†

Biochemistry ◽  
2000 ◽  
Vol 39 (21) ◽  
pp. 6275-6287 ◽  
Author(s):  
Richard J. Debus ◽  
Kristy A. Campbell ◽  
Donna P. Pham ◽  
Anna-Maria A. Hays ◽  
R. David Britt
Keyword(s):  
Photosystem Ii ◽  
Redox Properties ◽  
Manganese Cluster ◽  
D1 Polypeptide

scholarly journals 13C ENDOR Reveals That the D1 Polypeptide C-Terminus Is Directly Bound to Mn in the Photosystem II Oxygen Evolving Complex

2010 ◽  
Vol 132 (2) ◽  
pp. 446-447 ◽  
Author(s):  
Jamie A. Stull ◽  
Troy A. Stich ◽  
Rachel J. Service ◽  
Richard J. Debus ◽  
Sanjay K. Mandal ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Oxygen Evolving Complex ◽  
C Terminus ◽  
D1 Polypeptide ◽  
Oxygen Evolving

Does Aspartate 170 of the D1 Polypeptide Ligate the Manganese Cluster in Photosystem II? An EPR and ESEEM Study†

Biochemistry ◽  
2003 ◽  
Vol 42 (36) ◽  
pp. 10600-10608 ◽  
Author(s):  
Richard J. Debus ◽  
Constantino Aznar ◽  
Kristy A. Campbell ◽  
Wolfgang Gregor ◽  
Bruce A. Diner ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Manganese Cluster ◽  
D1 Polypeptide

scholarly journals Involvement of the HtrA family of proteases in the protection of the cyanobacterium Synechocystis PCC 6803 from light stress and in the repair of photosystem II

2002 ◽  
Vol 357 (1426) ◽  
pp. 1461-1468 ◽  
Author(s):  
Paulo Silva ◽  
Young–Jun Choi ◽  
Hanadi A. G. Hassan ◽  
Peter J. Nixon
Keyword(s):  
Photosystem Ii ◽  
Light Stress ◽  
High Light ◽  
Triple Mutant ◽  
D1 Polypeptide ◽  
In The Wild ◽  
D1 Turnover ◽  
Major Target ◽  
Pcc 6803

Photosystem II (PSII) is prone to irreversible light–induced damage, with the D1 polypeptide a major target. Repair processes operate in the cell to replace a damaged D1 subunit within the complex with a newly synthesized copy. As yet, the molecular details of PSII repair are relatively obscure despite the critical importance of this process for maintaining PSII activity and cell viability. We are using the cyanobacterium Synechocystis sp. PCC 6803 to identify the various proteases and chaperones involved in D1 turnover in vivo . Two families of proteases are being studied: the FtsH family (four members) of Zn 2+ –activated nucleotide–dependent proteases; and the HtrA (or DegP) family (three members) of serine–type proteases. In this paper, we report the results of our studies on a triple mutant in which all three copies of the htrA gene family have been inactivated. Growth of the mutant on agar plates was inhibited at high light intensities, especially in the presence of glucose. Oxygen evolution measurements indicated that, under conditions of high light, the rate of synthesis of functional PSII was less in the mutant than in the wild–type. Immunoblotting experiments conducted on cells blocked in protein synthesis further indicated that degradation of D1 was slowed in the mutant. Overall, our observations indicate that the HtrA family of proteases are involved in the resistance of Synechocystis 6803 to light stress and play a part, either directly or indirectly, in the repair of PSII in vivo .

Directed mutagenesis indicates that the donor to P 680+ in photosystem II is tyrosine-161 of the D1 polypeptide

Biochemistry ◽  
1988 ◽  
Vol 27 (26) ◽  
pp. 9071-9074 ◽  
Author(s):  
R. J. Debus ◽  
B. A. Barry ◽  
I. Sithole ◽  
Gerald T. Babcock ◽  
L. McIntosh
Keyword(s):  
Photosystem Ii ◽  
Directed Mutagenesis ◽  
D1 Polypeptide
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