Activity of a maize ubiquitin promoter in transgenic rice

1993 ◽  
Vol 23 (3) ◽  
pp. 567-581 ◽  
Author(s):  
Maria-Jes�s Cornejo ◽  
Diane Luth ◽  
Kathleen M. Blankenship ◽  
Olin D. Anderson ◽  
Ann E. Blechl
Keyword(s):  
Transgenic Rice ◽  
Ubiquitin Promoter ◽  
Maize Ubiquitin Promoter

scholarly journals Pollen-Specific CRISPR/Cas9 System to Increase Heritable Gene Mutations in Maize

Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 751
Author(s):  
Changshuang Jing ◽  
Min Wei ◽  
Peng Fang ◽  
Rentao Song ◽  
Weiwei Qi
Keyword(s):  
Gene Editing ◽  
Target Gene ◽  
Somatic Mutations ◽  
Gene Mutations ◽  
Plant Biotechnology ◽  
Bzip Transcription Factor ◽  
Ubiquitin Promoter ◽  
Independent Events ◽  
Large Numbers ◽  
Maize Ubiquitin Promoter

The CRISPR/Cas9 system has been widely utilized in plant biotechnology as a gene editing tool. However, a conventional design with ubiquitously expressed CRISPR/Cas9 was observed to cause large numbers of somatic mutations that complicated the identification of heritable mutations. We constructed a pollen-specific CRISPR/Cas9 (PSC) system using pollen-specific promoters of maize Profilin 1 and Profilin 3 (pZmPRO1 and pZmPRO3) to drive Cas9 expression, and the bZIP transcription factor Opaque2 (O2) was employed as the target gene. The maize ubiquitin promoter (pZmUbi)-driven CRISPR/Cas9 (UC) system was employed as a control. We generated transgenic plants for the PSC and UC systems and analyzed three independent events for each system. We found that the pZmPRO1 PSC system generated no target gene mutations in the T0 generation but successfully generated 0–90% target gene mutations in the T1 generation. A total of 31 of 33 mutations in the T1 generation could be inherited in the T2 generation. In addition, 88.9–97.3% of T2 mutations were from the T1 generation. The UC system generated mutations in the T0 generation, and 0%, 50% and 92.9% of T1 mutations were from the T0 generation. Our results demonstrate that the PSC system provided stable, heritable mutants in the next generation, and this approach might also be applied in other crops using germinal cell-specific CRISPR/Cas9 systems to facilitate plant breeding.

Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of GlutelinGluA-2in Endosperm of Transgenic Rice

2015 ◽  
Vol 41 (4) ◽  
pp. 524
Author(s):  
Hong-Mei WANG ◽  
Chang-Quan ZHANG ◽  
Qian-Feng LI ◽  
Samuel Sing-Min SUN ◽  
Qiao-Quan LIU ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Signal Peptide ◽  
Foreign Proteins

Cloning of New Herbicide Resistant Gene in Soil Metagenomics and Generation of Transgenic Rice Plants

2014 ◽  
Vol 40 (7) ◽  
pp. 1190
Author(s):  
Yun-Peng WANG ◽  
Jing-Yong MA ◽  
Rui MA ◽  
Jian MA ◽  
Wen-Guo LIU
Keyword(s):  
Transgenic Rice ◽  
Herbicide Resistant ◽  
Rice Plants ◽  
Resistant Gene ◽  
Transgenic Rice Plants ◽  
Soil Metagenomics
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