Characterization of rps17, rpl9 and rpl15: three nucleus-encoded plastid ribosomal protein genes

1992 ◽  
Vol 18 (5) ◽  
pp. 931-944 ◽  
Author(s):  
Michael D. Thompson ◽  
Colleen M. Jacks ◽  
Todd R. Lenvik ◽  
J. Stephen Gantt
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Protein Genes ◽  
Plastid Ribosomal Protein

scholarly journals Characterization of yeast strains with conditionally expressed variants of ribosomal protein genes tcm1 and cyh2

1985 ◽  
Vol 5 (1) ◽  
pp. 99-108
Author(s):  
H M Fried ◽  
H G Nam ◽  
S Loechel ◽  
J Teem
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Yeast Cells ◽  
Regulatory Sequence ◽  
Ribosomal Protein Genes ◽  
Wild Type ◽  
Yeast Strains ◽  
Reduced Rate ◽  
Hybrid Genes

We placed a regulatory sequence derived from the GAL10 locus of Saccharomyces cerevisiae at various distances from the start sites of transcription of two yeast ribosomal protein genes, tcm1 and cyh2. The hybrid ribosomal protein genes were transcribed at wild-type levels in the presence of galactose. In the absence of galactose, the hybrid genes were transcribed either at a reduced level or essentially not at all. Yeast cells which transcribe the ribosomal protein genes at a reduced rate continued to grow, suggesting that enhanced translation of the ribosomal protein mRNA may permit an adequate rate of synthesis of the corresponding protein. Consistent with this suggestion is the finding that preexisting mRNA decayed at a reduced rate when transcription was halted abruptly by removal of galactose. Yeast cells unable to transcribe tcm1 or cyh2 without galactose did not grow. These conditional lethal strains demonstrate that the ribosomal proteins encoded by tcm1 and cyh2 are essential; furthermore, these strains are potentially useful for isolating mutations in the tcm1 and cyh2 proteins affecting their transport, assembly, or function.

Discovery of Over-Represented Words in Intron 1s of Drosophila Ribosomal Protein Genes

2014 ◽  
Vol 707 ◽  
pp. 117-120
Author(s):  
Hui Min Li ◽  
Zhi Gang Yang ◽  
Dan Chen
Keyword(s):  
Transcriptional Regulation ◽  
Ribosomal Protein ◽  
Dna Sequences ◽  
Binding Sites ◽  
Regulatory Elements ◽  
Score Statistic ◽  
Z Score ◽  
Biological Functions ◽  
Ribosomal Protein Genes

Most of studies on transcriptional regulation mainly focus on upstream regions of genes. More and more recent researches indicate that introns may have important biological functions in transcription regulation of genes. The characterization of words in DNA sequences can be facilitated by the sequences’ functions. Using U-score and Z-score statistic, respectively, we extracted some over-represented words in intron 1s of ribosomal protein genes. A majority of them are accordance with known transcriptional factor binding sites and are potential regulatory elements. And, the detected over-represented words are more likely to form wider potential sequences and are denser in intron 1s of RP genes. We speculate the properties of these words may be associated with the transcriptional regulation of RP genes.

scholarly journals Characterization of yeast strains with conditionally expressed variants of ribosomal protein genes tcm1 and cyh2.

1985 ◽  
Vol 5 (1) ◽  
pp. 99-108 ◽  
Author(s):  
H M Fried ◽  
H G Nam ◽  
S Loechel ◽  
J Teem
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Yeast Cells ◽  
Regulatory Sequence ◽  
Ribosomal Protein Genes ◽  
Wild Type ◽  
Yeast Strains ◽  
Reduced Rate ◽  
Hybrid Genes

We placed a regulatory sequence derived from the GAL10 locus of Saccharomyces cerevisiae at various distances from the start sites of transcription of two yeast ribosomal protein genes, tcm1 and cyh2. The hybrid ribosomal protein genes were transcribed at wild-type levels in the presence of galactose. In the absence of galactose, the hybrid genes were transcribed either at a reduced level or essentially not at all. Yeast cells which transcribe the ribosomal protein genes at a reduced rate continued to grow, suggesting that enhanced translation of the ribosomal protein mRNA may permit an adequate rate of synthesis of the corresponding protein. Consistent with this suggestion is the finding that preexisting mRNA decayed at a reduced rate when transcription was halted abruptly by removal of galactose. Yeast cells unable to transcribe tcm1 or cyh2 without galactose did not grow. These conditional lethal strains demonstrate that the ribosomal proteins encoded by tcm1 and cyh2 are essential; furthermore, these strains are potentially useful for isolating mutations in the tcm1 and cyh2 proteins affecting their transport, assembly, or function.

scholarly journals A Drosophila third chromosome Minute locus encodes a ribosomal protein.

Genetics ◽  
1994 ◽  
Vol 137 (2) ◽  
pp. 513-520 ◽  
Author(s):  
S Andersson ◽  
S Saebøe-Larssen ◽  
A Lambertsson ◽  
J Merriam ◽  
M Jacobs-Lorena
Keyword(s):  
Ribosomal Protein ◽  
Amino Acid Level ◽  
P Element ◽  
Nucleotide Sequence Analysis ◽  
Ribosomal Protein Genes ◽  
Inverse Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Ribosomal Protein S3 ◽  
Polymerase Chain

Abstract Minutes (M) are a group of over 50 phenotypically similar Drosophila mutations widely believed to affect ribosomal protein genes. This report describes the characterization of the P element-induced M(3)95A(Plac92) mutation [allelic to M(3)95A]. This mutation can be reversed by the mobilization of the P element, demonstrating that the mutation is caused by insertion of this transposable element. The gene interrupted by insertion of the P element was cloned by use of inverse polymerase chain reaction. Nucleotide sequence analysis revealed a 70-75% identity to the human and rat ribosomal protein S3 genes, and to the Xenopus ribosomal protein S1a gene. At the amino acid level, the overall identity is approximately 78% for all three species. This is only the second time that a Minute has been demonstrated to encode a ribosomal protein.

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