Nucleic acid sequence of a 21 kDa cocoa seed protein with homology to the soybean trypsin inhibitor (Kunitz) family of protease inhibitors

1991 ◽  
Vol 16 (5) ◽  
pp. 913-915 ◽  
Author(s):  
Heeyoung Tai ◽  
Lauren McHenry ◽  
Paul J. Fritz ◽  
Douglas B. Furtek
Keyword(s):  
Nucleic Acid ◽  
Protease Inhibitors ◽  
Trypsin Inhibitor ◽  
Seed Protein ◽  
Soybean Trypsin Inhibitor ◽  
Nucleic Acid Sequence ◽  
Kunitz Family

Inhibition of Chorion Expansion and Preservation of Fertility in Goldfish (Carassius auratus) Eggs by Protease Inhibitors

1993 ◽  
Vol 50 (5) ◽  
pp. 932-935 ◽  
Author(s):  
Sheau-Yu Hsu ◽  
Frederick William Goetz
Keyword(s):  
Protease Inhibitors ◽  
Trypsin Inhibitor ◽  
Carassius Auratus ◽  
Ringer Solution ◽  
Soybean Trypsin Inhibitor ◽  
Ringer’S Solution ◽  
Goldfish Carassius Auratus ◽  
Preservation Of Fertility ◽  
In Vitro Effects

The in vitro effects of protease inhibitors on chorion expansion and the fertility of goldfish (Carassius auratus) eggs were investigated. Expansion of the chorion in ovulated eggs was blocked by the serine protease inhibitors benzamidine and soybean trypsin inhibitor when they were held in a goldfish Ringer solution. Eggs in which chorion expansion was inhibited retained their fertility for 30 min when held in this solution. In contrast, ovulated eggs lost their fertility in freshwater even in the presence of a protease inhibitor. The results suggest that chorion expansion and loss of fertility in water-activated goldfish eggs may involve proteolysis which was blocked by benzamidine and soybean trypsin inhibitor. Moreover, the blockage of chorion expansion may be pivotal to the preservation of fertility in eggs held in Ringer's solution with soybean trypsin inhibitor. The development of this solution provides a means to temporarily hold ovulated goldfish eggs in a viable state outside the female and will permit further studies on fertilization in this species.

Factor X Activation by washed human platelets

1979 ◽  
Author(s):  
E van Wijk ◽  
L Kahlé ◽  
J ten Cate
Keyword(s):  
Human Factors ◽  
Trypsin Inhibitor ◽  
Calcium Ions ◽  
Factor Xa ◽  
Human Platelets ◽  
Soybean Trypsin Inhibitor ◽  
Thrombin Inhibitor ◽  
Chromogenic Substrate ◽  
Factor X ◽  
Factor X Activation

In a system of washed human platelets, Ca2+and purified human factors X anc II, a sufficient amount of thrombin is generated in about 10 minutes to aggregate the platelets. This thrombin is formed through the activation of FX by the platelets. In a system with either FX or FII present, no aggregation occurs. In addition no aggregation is observed when hirudin, a specific thrombin inhibitor, or when soybean trypsin inhibitor, which inhibits factor Xa, are added to the mixture. The formation of factor Xa can be monitored indirectly through the generation of thrombin, in the presence of an excess of prothrombin, using a thrombin sensitive chromogenic substrate. When washed platelets are incubated with FX alone for 10 minutes, no aggregation occurs and after the addition of prothrombin aggregation starts within 6 minutes. These findings confirm that washed platelets possess a factor X activating property. The generation of FXa proceeds in the absence of added Ca2+, whereas in the presence of Ca2+factor Xa activity reaches a maximum in 3 minutes, whereafter the activity progressively decreases. This may be due to the binding of Xa to the platelets in the presence of calcium ions.

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

Rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification

2015 ◽  
Vol 160 (3) ◽  
pp. 719-725 ◽  
Author(s):  
Qiu-Hua Mo ◽  
Hai-Bo Wang ◽  
Hui-Rong Dai ◽  
Ji-Can Lin ◽  
Hua Tan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Simultaneous Detection ◽  
Nucleic Acid Sequence
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