Stress responses in alfalfa (Medicago sativa L.). 8. Cis-elements and trans-acting factors for the quantitative expression of a bean chalcone synthase gene promoter in electroporated alfalfa protoplasts

1991 ◽  
Vol 16 (5) ◽  
pp. 877-890 ◽  
Author(s):  
Maria J. Harrison ◽  
Arvind D. Choudhary ◽  
Ian Dubery ◽  
Christopher J. Lamb ◽  
Richard A. Dixon
Keyword(s):  
Medicago Sativa ◽  
Stress Responses ◽  
Chalcone Synthase ◽  
Gene Promoter ◽  
Cis Elements ◽  
Quantitative Expression ◽  
Chalcone Synthase Gene ◽  
Medicago Sativa L

scholarly journals Stress Responses in Alfalfa (Medicago sativa L.)

1990 ◽  
Vol 94 (4) ◽  
pp. 1802-1807 ◽  
Author(s):  
Arvind D. Choudhary ◽  
Christopher J. Lamb ◽  
Richard A. Dixon
Keyword(s):  
Medicago Sativa ◽  
Stress Responses ◽  
Medicago Sativa L

scholarly journals Multi-tissue transcriptome analysis of two Begonia species reveals dynamic patterns of evolution in the chalcone synthase gene family

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Katie Emelianova ◽  
Andrea Martínez Martínez ◽  
Lucia Campos-Dominguez ◽  
Catherine Kidner
Keyword(s):  
Gene Duplication ◽  
Gene Family ◽  
Genome Size ◽  
Stress Responses ◽  
Chalcone Synthase ◽  
Expression Profiles ◽  
Rna Seq ◽  
Biotic And Abiotic Stress ◽  
Chalcone Synthase Gene ◽  
Study Species

AbstractBegonia is an important horticultural plant group, as well as one of the most speciose Angiosperm genera, with over 2000 described species. Genus wide studies of genome size have shown that Begonia has a highly variable genome size, and analysis of paralog pairs has previously suggested that Begonia underwent a whole genome duplication. We address the contribution of gene duplication to the generation of diversity in Begonia using a multi-tissue RNA-seq approach. We chose to focus on chalcone synthase (CHS), a gene family having been shown to be involved in biotic and abiotic stress responses in other plant species, in particular its importance in maximising the use of variable light levels in tropical plants. We used RNA-seq to sample six tissues across two closely related but ecologically and morphologically divergent species, Begonia conchifolia and B. plebeja, yielding 17,012 and 19,969 annotated unigenes respectively. We identified the chalcone synthase gene family members in our Begonia study species, as well as in Hillebrandia sandwicensis, the monotypic sister genus to Begonia, Cucumis sativus, Arabidopsis thaliana, and Zea mays. Phylogenetic analysis suggested the CHS gene family has high duplicate turnover, all members of CHS identified in Begonia arising recently, after the divergence of Begonia and Cucumis. Expression profiles were similar within orthologous pairs, but we saw high inter-ortholog expression variation. Sequence analysis showed relaxed selective constraints on some ortholog pairs, with substitutions at conserved sites. Evidence of pseudogenisation and species specific duplication indicate that lineage specific differences are already beginning to accumulate since the divergence of our study species. We conclude that there is evidence for a role of gene duplication in generating diversity through sequence and expression divergence in Begonia.

scholarly journals Stress Responses in Alfalfa (Medicago sativa L.)

1990 ◽  
Vol 92 (2) ◽  
pp. 447-455 ◽  
Author(s):  
Jesus Jorrin ◽  
Richard A. Dixon
Keyword(s):  
Medicago Sativa ◽  
Stress Responses ◽  
Medicago Sativa L

scholarly journals Stress Responses in Alfalfa (Medicago sativa L.)

1990 ◽  
Vol 94 (1) ◽  
pp. 227-232 ◽  
Author(s):  
Helmut Kessmann ◽  
Robert Edwards ◽  
Paul W. Geno ◽  
Richard A. Dixon
Keyword(s):  
Medicago Sativa ◽  
Stress Responses ◽  
Medicago Sativa L

scholarly journals UV-inducible transient expression in parsley protoplasts identifies regulatory cis -elements of a chimeric Antirrhinum majus chalcone synthase gene

1988 ◽  
Vol 7 (13) ◽  
pp. 4027-4033 ◽  
Author(s):  
Susanne Lipphardt ◽  
Reinhold Brettschneider ◽  
Fritz Kreuzaler ◽  
Jeff Schell ◽  
Jeffery L. Dangl
Keyword(s):  
Transient Expression ◽  
Chalcone Synthase ◽  
Antirrhinum Majus ◽  
Cis Elements ◽  
Chalcone Synthase Gene
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