Small-scale field tests with transgenic potato, cv. Bintje, to test resistance to primary and secondary infections with potato virus y

1994 ◽  
Vol 25 (6) ◽  
pp. 963-975 ◽  
Author(s):  
Pia Malno� ◽  
Laurent Farinelli ◽  
Gerald F. Collet ◽  
Werner Reust
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Field Tests ◽  
Transgenic Potato ◽  
Small Scale ◽  
Secondary Infections ◽  
Scale Field

scholarly journals Evaluation of Energy Recovery from Laboratory Experiments and Small-scale Field Tests of Underground Coal Gasification (UCG)

2015 ◽  
Vol 131 (5) ◽  
pp. 203-218 ◽  
Author(s):  
Faqiang SU ◽  
Ken-ichi ITAKURA ◽  
Gota DEGUCHI ◽  
Koutarou OHGA ◽  
Mamoru KAIHO
Keyword(s):  
Energy Recovery ◽  
Laboratory Experiments ◽  
Coal Gasification ◽  
Field Tests ◽  
Small Scale ◽  
Underground Coal Gasification ◽  
Scale Field

scholarly journals Extreme resistance to Potato Virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor

2018 ◽  
Author(s):  
Marta Grech-Baran ◽  
Kamil Witek ◽  
Katarzyna Szajko ◽  
Agnieszka I Witek ◽  
Karolina Morgiewicz ◽  
...  
Keyword(s):  
Single Molecule ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Resistance Breeding ◽  
Transgenic Potato ◽  
Extreme Resistance ◽  
Breeding Programs ◽  
Potato Plants ◽  
Gene Enrichment ◽  
Transgenic Potato Plants

ABSTRACTPotato virus Y (PVY) is a major potato pathogen that causes annual losses of billions of dollars. Control of its transmission requires extensive use of environmentally damaging insecticides. Rysto confers extreme resistance (ER) to PVY and is a valuable trait in resistance breeding programs. We isolated Rysto using Resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences Single-Molecule Real Time Sequencing). Rysto encodes a nucleotide binding-leucine rich repeat (NLR) protein with an N-terminal TIR domain, and is sufficient for PVY perception and extreme resistance in transgenic potato plants. We investigated the requirements for Rysto-dependent extreme resistance, and showed that Rysto function is temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.

scholarly journals Resistance levels to two strains of Potato virus Y (PVY) in transgenic potatoes cv. Achat

2001 ◽  
Vol 19 (3) ◽  
pp. 348-350 ◽  
Author(s):  
André N. Dusi ◽  
César Carvalho ◽  
Antônio Carlos Torres ◽  
Antonio Carlos de Ávila
Keyword(s):  
Transgenic Plants ◽  
Optical Density ◽  
Potato Virus ◽  
Partial Resistance ◽  
Potato Virus Y ◽  
Systemic Infection ◽  
Transgenic Potato ◽  
Extreme Resistance ◽  
Density Values ◽  
Transgenic Potatoes

Two transgenic potato clones of cv. Achat, denominated 1P and 63P were challenged with two Potato virus Y strains (PVY O and PVY N), under greenhouse conditions, to be evaluated for resistance to these strains. Optical density values of the Elisa readings of samples from the transgenic plants were compared to readings from samples of the inoculated non-transformed plants. Clone 1P was extremely resistant to both PVY strains, reflected by not being systemically infected. Clone 63P, however, presented partial resistance to both PVY strains as local or systemic infection was delayed in some days. These results confirm the previously reported extreme resistance to PVY of clone 1P.

scholarly journals Potential of an Indigenous Fungus, Plectosporium tabacinum, as a Mycoherbicide for Control of Arrowhead (Sagittaria trifolia)

Plant Disease ◽  
1998 ◽  
Vol 82 (6) ◽  
pp. 657-660 ◽  
Author(s):  
Young Ryun Chung ◽  
Suk Jin Koo ◽  
Heung Tae Kim ◽  
Kwang Yun Cho
Keyword(s):  
Foliar Spray ◽  
Field Tests ◽  
Potato Dextrose Agar ◽  
Small Scale ◽  
Conidial Suspension ◽  
Corn Meal ◽  
Weed Species ◽  
Potato Dextrose Broth ◽  
Leaf Stage ◽  
Scale Field

An isolate of the indigenous fungus Plectosporium tabacinum was isolated from arrowhead (Sagittaria trifolia) in Yusung, Korea in 1990 and evaluated in laboratory and growth chamber tests as a potential mycoherbicide. The fungus grew comparatively slowly on potato dextrose agar and corn meal agar, attaining a diameter of 65 mm after 12 days at 25°C. Conidia were mass-produced in shake-cultures or in a fermentor using potato dextrose broth containing yeast extract (0.5%, wt/vol) at 25°C. When arrowhead seedlings at the 2- to 3-leaf stage were inoculated with conidial suspensions (2 × 107 conidia/ml) and incubated in a dew chamber for 18 h at 25°C, the plants developed small, brown spots on the leaves and petioles in 2 days, and were blighted completely within 7 days after inoculation. This effect was consistent on arrowhead plants from the 2- to 5-leaf stage. Another arrowhead species, S. pygmaea, was as susceptible as S. trifolia to the pathogen. Several crops, including rice, barley, and wheat and 34 other common weed species, were immune. In small-scale field tests in paddy fields during the summers of 1992 and 1993, a mean reduction of 71.3% in the number of arrowhead plants was observed following a foliar spray of a conidial suspension (107 conidia/ml). These results indicate that P. tabacinum has potential as a selective mycoherbicide for arrowhead control.

StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with Potato virus Y

2021 ◽  
Author(s):  
Max M Combest ◽  
Natalia Moroz ◽  
Kiwamu Tanaka ◽  
Conner J Rogan ◽  
Jeffrey C Anderson ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Plant Defense ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Systemic Infection ◽  
Transgenic Potato ◽  
Defense Responses ◽  
Marker Genes ◽  
Plant Defense Responses ◽  
Callose Deposition

Abstract The role of small secreted peptides in plant defense responses to viruses has seldom been investigated. Here, we report a role for potato (Solanum tuberosum) PIP1, a gene predicted to encode a member of the pathogen-associated molecular pattern (PAMP)-induced peptide (PIP) family, in the response of potato to Potato virus Y (PVY) infection. We show that exogenous application of synthetic StPIP1 to potato leaves and nodes increased the production of reactive oxygen species and the expression of plant defense-related genes, revealing that StPIP1 triggers early defense responses. In support of this hypothesis, transgenic potato plants that constitutively overexpress StPIP1 had higher levels of leaf callose deposition and, based on measurements of viral RNA titers, were less susceptible to infection by a compatible PVY strain. Interestingly, systemic infection of StPIP1-overexpressing lines with PVY resulted in clear rugose mosaic symptoms that were absent or very mild in infected non-transgenic plants. A transcriptomics analysis revealed that marker genes associated with both pattern-triggered immunity and effector-triggered immunity were induced in infected StPIP1 overexpressors but not in non-transgenic plants. Together, our results reveal a role for StPIP1 in eliciting plant defense responses and in regulating plant antiviral immunity.

scholarly journals Detection of Potato virus Y associated with African nightshade leafy vegetable (Solanum scabrum miller) in Kenya

2020 ◽  
Vol 12 (3) ◽  
pp. 255-259
Author(s):  
S.L. Kimaru ◽  
D.C. Kilalo ◽  
J.W. Kimenju ◽  
W.M. Muiru
Keyword(s):  
Potato Virus ◽  
Food Systems ◽  
Potato Virus Y ◽  
Plant Viruses ◽  
Molecular Techniques ◽  
Sub Saharan Africa ◽  
Small Scale ◽  
Molecular Testing ◽  
Ecological Zones ◽  
Field Samples

Abstract. The African nightshades (ANS) have been part of the food systems in sub-Saharan Africa for generations. They are particularly attractive to small scale farmers because the risks of crop losses are much lower compared to the exotic vegetables. Plant viruses are economically important pathogens affecting African nightshade production. More than 200 plant viruses are reported to infest solanaceous family crops. This study was carried out to detect Potato virus Y associated with African nightshade. Symptomatic leaf samples were obtained from four agro-ecological zones in Kenya. The viral pathogens were detected using serology and molecular techniques. Laboratory experiments were carried out at the University of Nairobi and Kenya Plant Health Inspectorate Service (KEPHIS) from February 2017 to August, 2018. Field samples had the highest mean viral percentage frequency of 44.8% compared to greenhouse samples having 29.8% using serology methods. Molecular testing revealed that greenhouse and farm field sample were positive for the three Potato virus Y strains PVYN:O (necrotic recombinant), PVYO (ordinary) and PVYNTN (necrotic). Detection of Potato virus Y in ANS suggests that it is prevalent in the sampled regions and could cause problems to other solanaceous crops.

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