Identification of a maize root transcript expressed in the primary response to nitrate: characterization of a cDNA with homology to ferredoxin-NADP+ oxidoreductase

1994 ◽  
Vol 26 (2) ◽  
pp. 679-690 ◽  
Author(s):  
Steven W. Ritchie ◽  
Margaret G. Redinbaugh ◽  
Naomasa Shiraishi ◽  
Jacqueline M. Vrba ◽  
Wilbur H. Campbell
Keyword(s):  
Primary Response ◽  
Maize Root

scholarly journals BIOLOGICAL CHARACTERIZATION OF AN IMMUNOSUPPRESSANT FROM GROUP A STREPTOCOCCI

1971 ◽  
Vol 134 (5) ◽  
pp. 1253-1265 ◽  
Author(s):  
Artin H. Malakian ◽  
John H. Schwab
Keyword(s):  
Antibody Formation ◽  
Memory Cell ◽  
Primary Response ◽  
Repeated Injection ◽  
Group A Streptococci ◽  
Biological Characterization ◽  
Protein Antigens ◽  
Immunosuppressive Action ◽  
Group A

A component in extracts of Group A streptococci suppresses antibody formation in mice against heterologous erythrocyte and protein antigens. Large doses are not toxic and repeated injection does not change its effectiveness. It is most effective when injected 1 or 2 days before antigen and it is not suppressive when given after antigen. The active factor occurs as a large polydisperse complex and activity can be increased 10- to 25-fold by filtration through Sepharose 2B. Both direct (γM) and indirect (γG) antibody-forming cells are suppressed in primary and secondary responses. Injection before a primary response does not reduce memory cell development. It increases rather than depresses the "background" antibody-forming cells to sheep erythrocytes, and is equally effective if injected intraperitoneally or intravenously. Ribonuclease increases activity while deoxyribonuclease has no effect. Proteases destroy immunosuppressive action.

Tomato bushy stunt virus from Prunus avium. II. Serological typing and the characterization of antibody types and activities

1968 ◽  
Vol 46 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Wayne R. Allen
Keyword(s):  
Type Strain ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Tomato Bushy Stunt Virus ◽  
Cross Reactivity ◽  
Primary Response ◽  
Maximum Value ◽  
Serological Typing ◽  
Maximum Titer

Serological comparisons between the type strain of tomato bushy stunt virus and a strain from sweet cherry demonstrated that the degree of relationship was highly dependent upon the time of bleeding during the primary response. Cross-reactivity decreased after injection until just before the maximum titer was reached, and then gradually increased until the maximum value was attained. The increase in cross-reactivity was due, mainly, to a decline in synthesis and (or) release of specific antibodies. Sera fractionation revealed specific and cross-reactive antibodies among both light and heavy globulins. The cross-reactivity of light antibodies increased from the time of injection, but it remained nearly constant for heavy antibodies. Immunoelectrophoresis associated the light and heavy antibodies with IgG and IgM globulins, respectively.

scholarly journals Characterization of a Maize Root Proteinase

1993 ◽  
Vol 101 (2) ◽  
pp. 415-419 ◽  
Author(s):  
V. J. Goodfellow ◽  
L. P. Solomonson ◽  
A. Oaks
Keyword(s):  
Maize Root

Characterization of phosphate absorption in maize root cortex segments

1983 ◽  
Vol 25 (5) ◽  
pp. 366-372 ◽  
Author(s):  
Ivana Macháčková ◽  
J. Král ◽  
Z. Zmrhal
Keyword(s):  
Maize Root ◽  
Root Cortex ◽  
Phosphate Absorption

Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding

PROTOPLASMA ◽  
2014 ◽  
Vol 252 (1) ◽  
pp. 335-343 ◽  
Author(s):  
Vesna Hadži-Tašković Šukalović ◽  
Mirjana Vuletić ◽  
Ksenija Marković ◽  
Tijana Cvetić Antić ◽  
Željko Vučinić
Keyword(s):  
Cell Walls ◽  
Biochemical Characterization ◽  
Covalent Binding ◽  
Root Cell ◽  
Maize Root ◽  
Enzyme Properties ◽  
Class Iii ◽  
Root Cell Walls

Serological characterization of γM (19 S) and γG (7 S) rabbit antibodies in response to Entamoeba histolytica antigens

1968 ◽  
Vol 14 (12) ◽  
pp. 1317-1323 ◽  
Author(s):  
Z. Ali Khan ◽  
E. Meerovitch
Keyword(s):  
Entamoeba Histolytica ◽  
Primary Response ◽  
Complete Freund’S Adjuvant ◽  
Secondary Response ◽  
Antibody Activity ◽  
Serological Characterization ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Entamoeba histolytica (DKB strain) antigen mixed with an equal volume of complete Freund's adjuvant was injected at multiple sites into three rabbits. Primary response sera were drawn 1, 2, and 3 weeks after the first injection. Two injections of antigen were given in the fourth week, at 3-day intervals. The secondary response sera were drawn 5, 6, and 12 weeks after the beginning of immunization. A steady hemagglutination (HA) titer of 1:128 was obtained with all the three primary response sera, while the secondary response sera showed HA titers of 1:2000 to 1:8000. The 0.1 M 2-mercaptoethanol treatment of these sera reduced, but did not completely obliterate, their HA activity. The HA activity was detected in both the pooled γM and γG classes of immunoglobulins of the primary and secondary response sera; the immunoprecipitating activity was confined to the γG class. 2-Mercaptoethanol treatment completely destroyed the antibody activity of γM fractions without affecting that of the γG fraction. Repeated immunoelectrophoresis runs of the whole sera and pooled γM and γG fractions showed variable numbers of precipitin bands at the γG position but none at the γM and γA positions.

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