Competition between a native isolate of Rhizobium leguminosarum bv trifolii and two commercial inoculant strains for nodulation of clover

1991 ◽  
Vol 137 (2) ◽  
pp. 293-296 ◽  
Author(s):  
E. Fabiano ◽  
A. Arias
Keyword(s):  
Rhizobium Leguminosarum ◽  
Commercial Inoculant

Symbiotic effectiveness of Rhizobium leguminosarum bv. trifolii collected from pastures in south-western Victoria

1997 ◽  
Vol 37 (6) ◽  
pp. 623 ◽  
Author(s):  
P. E. Quigley ◽  
P. J. Cunningham ◽  
M. Hannah ◽  
G. N. Ward ◽  
T. Morgan
Keyword(s):  
Rhizobium Leguminosarum ◽  
Subterranean Clover ◽  
Annual Rainfall ◽  
Test Plant ◽  
Available Phosphorus ◽  
Symbiotic Effectiveness ◽  
Plant Weight ◽  
Commercial Inoculant ◽  
Shoot Weight ◽  
Western Victoria

Summary. The whole-soil inoculation method was used to assess the symbiotic effectiveness of rhizobia populations in soils collected from 18 randomly-selected pastures in south-western Victoria. This was part of a larger study which described the condition of pasture within this region. Based on the shoot weights of test subterranean clover plants, cv. Mount Barker, effectiveness varied from 36 to 94% depending on the site of rhizobia collection. This range was wider than that found in an earlier survey of rhizobia effectiveness conducted nearby. WU95, the commercial inoculant for subterranean clover, was significantly more effective than 9 of the rhizobia samples. Rhizobia from 2 sites were especially poor and their effectiveness (37%) was not significantly different from the nil inoculum control (28%). Symbiotic effectiveness was not related to soil pH, available sulfur, available phosphorus, total nitrogen or mean annual rainfall for each site where rhizobia were collected. After pooling data for all sites, the shoot weights were significantly related to the proportions of plants with nodules assigned high nodulation scores. In contrast, low scores, within 1 of 6 categories, did not significantly affect shoot weight. The technique of using mean nodulation score was less capable of discriminating differences in symbiotic effectiveness, compared with assessment based on test plant weight.

Persistence of introduced strains of Rhizobium leguminosarum bv trifolii in acidic soils of north-eastern Victoria

1999 ◽  
Vol 39 (7) ◽  
pp. 829 ◽  
Author(s):  
J. F. Slattery ◽  
D. R. Coventry
Keyword(s):  
Rhizobium Leguminosarum ◽  
Soil Amendments ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Nodule Occupancy ◽  
Acidic Soils ◽  
High Background ◽  
Poor Tolerance ◽  
Acidic Sites ◽  
Commercial Inoculant

Summary. A 5-year study was undertaken to establish if introduced rhizobia with higher tolerance to Al than the current inoculant Rhizobium can persist and continue nodulating subterranean clover (Trifolium subterraneum L.) in acidic soils. Two Rhizobium leguminosarum bv trifolii strains were introduced as seed inoculants with subterranean clover at 2 acidic sites (pHCa 4.1 and pHCa 4.3), where lime and gypsum had been applied as soil amendments. Strain NA3001 was selected for its tolerance to high Al concentrations when grown on an agar medium and WU95, which is a widely used commercial inoculant strain, for its relatively poor tolerance to Al when grown on agar. Liming the soil increased its pH and reduced the concentration of extractable Al at both sites. In the year the subterranean clover was sown, strain WU95 had nodule occupancy of 20–49%, decreasing with time to 4–7% after 5 seasons (1991–95). The nodule occupancy of strain NA3001 was initially lower than strain WU95 (14–16%), but its occupancy did not vary with time (significant strain x time interactions, P<0.05). These data indicate that the acid-tolerant strain NA3001 has the potential to persist in these strongly acidic soils and, despite the presence of high background populations of naturalised rhizobia, to continue initiating nodulation. The use of soil amendments (lime and gypsum) to increase pH and reduce soluble Al concentrations did not affect the nodule occupancy of either NA3001 or WU95 with time, nor did it slow the rate of decline in nodule occupancy of WU95.

scholarly journals Complete Genome Sequences of Trifolium spp. Inoculant Strains Rhizobium leguminosarum sv. trifolii TA1 and CC275e: Resources for Genomic Study of the Rhizobium-Trifolium Symbiosis

2020 ◽  
pp. MPMI-08-20-0220
Author(s):  
Benjamin J. Perry ◽  
Shaun Ferguson ◽  
Aurelie Laugraud ◽  
Steve A. Wakelin ◽  
Wayne Reeve ◽  
...  
Keyword(s):  
New Zealand ◽  
Open Access ◽  
Complete Genome ◽  
Rhizobium Leguminosarum ◽  
Nitrogen Fixing ◽  
Genome Sequences ◽  
Future Studies ◽  
Genomic Study ◽  
Commercial Inoculant ◽  
Open Access Article

Rhizobium leguminosarum symbiovar trifolii strains TA1 and CC275e are nitrogen-fixing microsymbionts of Trifolium spp. and have been used as commercial inoculant strains for clovers in pastoral agriculture in Australia and New Zealand. Here we present the complete genome sequences of both strains, resolving their multipartite genome structures and allowing for future studies using genomic approaches. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

Nodulation and growth of pasture legumes with naturalised soil rhizobia.2. Balansa clover (Trifolium michelianum Savi)

2002 ◽  
Vol 42 (7) ◽  
pp. 939 ◽  
Author(s):  
R. A. Ballard ◽  
A. D. Craig ◽  
N. Charman
Keyword(s):  
Rhizobium Leguminosarum ◽  
Widespread Occurrence ◽  
Pasture Legumes ◽  
Cultivar Selection ◽  
Eastern Australia ◽  
Commercial Cultivars ◽  
Commercial Inoculant ◽  
Inoculant Strain ◽  
History Of ◽  
Trifolium Michelianum

Balansa clover (Trifolium michelianum Savi) is grown widely across southern and eastern Australia. The presence of suitable rhizobia (Rhizobium leguminosarum bv. trifolii) for this species of clover, and their ability to form an effective symbiosis, was assessed in a survey of 43 soils collected from across Australia. The effectiveness of the soil rhizobia is compared with that of strains WU95 and WSM409. The study confirmed the widespread occurrence of clover rhizobia in Australian soils. No clover rhizobia were detected in 6 of the 43 soils and this was probably associated with a history of clover absence at these soil locations. Thirty of the soils contained more than 500 rhizobia per gram. These rhizobia varied from highly effective for soil T2 (128% of WSM409) through to ineffective for soil V9 (22% of WSM409). Few soil inoculants were more effective than strain WSM409. Commercial inoculant strain WSM409 was generally more effective than former inoculant strain WU95.The suboptimal performance of the rhizobia in many of the soils indicates that there is an opportunity to improve the level of nitrogen fixation by balansa clover in the field. Bearing in mind the difficulties associated with the introduction and persistence of applied inoculant strains, cultivar selection is considered as an approach to improve the symbiosis. However, this study indicated little variation in the performance of the 3 commercial cultivars, Frontier, Paradana and Bolta, in this regard.

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