Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

1995 ◽  
Vol 14 (6) ◽  
pp. 489-500 ◽  
Author(s):  
William King ◽  
David L. Berlinsky ◽  
Craig V. Sullivan
Keyword(s):  
Oocyte Maturation ◽  
White Perch ◽  
Gonadal Steroids ◽  
In Vitro Studies ◽  
Morone Americana ◽  
White Bass ◽  
Final Oocyte Maturation

Effects of gonadal steroids and human chorionic gonadotrophin on final oocyte maturation in vitro in the New Zealand snapper Pagrus auratus (Sparidae)

1995 ◽  
Vol 46 (2) ◽  
pp. 467 ◽  
Author(s):  
AR Ventling ◽  
NW Pankhurst
Keyword(s):  
New Zealand ◽  
Oocyte Maturation ◽  
Ovarian Tissue ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Gonadal Steroids ◽  
Response To Treatment ◽  
Final Oocyte Maturation ◽  
Pagrus Auratus

This study investigated the role of steroids and gonadotrophin in final oocyte maturation (FOM) in the New Zealand snapper (Pagrus auratus) by means of in vitro techniques. Ovarian fragments containing vitellogenic oocytes were incubated for up to 48 h with a range of doses of gonadal steroids andlor human chorionic gonadotrophin (hCG). The ability of ovarian tissue to synthesize putative maturational steroids was assessed by measuring production of 17α,20β-dihydroxy-4-pregnen- 3-one (17,20βP) by ovarian fragments incubated with hCG and 17α-hydroxyprogesterone (17P). 17,20βP was consistently the most effective maturational steroid, inducing FOM at doses as low as 1 ng mI-1. The next most potent steroids were 20β-hydroxylated progestins and 17P, which were effective at relatively high doses (100 ng mI-1) only. The single androgen tested (testosterone) was least effective at inducing FOM. Oocytes were less responsive to hCG alone than to steroids, but the combination of hCG and steroids enhanced responsiveness in some incubations. Oocytes produced 17,20βP in response to treatment with 17P, and the response was augmented by co-treatment with hCG. HCG alone was ineffective at stimulating production of 17,20βP. The results of this study are consistent with reports that 17,20βP is generally the most potent steroid for the induction of FOM in teleosts.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

Ciamexon in low-dose streptozotocin induced diabetes mellitus. In vivo and in vitro studies

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S120-S121
Author(s):  
TH. LINN ◽  
H. GERMANN ◽  
B. HERING ◽  
R. BRETZEL ◽  
K. FEDERLIN
Keyword(s):  
Diabetes Mellitus ◽  
Low Dose ◽  
In Vitro Studies ◽  
Streptozotocin Induced Diabetes

Delayed hypersensitivity to tetanus toxoid in man: in vivo and in vitro studies

Pathology ◽  
1983 ◽  
Vol 15 (4) ◽  
pp. 369-372 ◽  
Author(s):  
Christine Johnson ◽  
R.S. Walls ◽  
A. Ruwoldt
Keyword(s):  
Tetanus Toxoid ◽  
In Vitro Studies ◽  
Delayed Hypersensitivity
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