Purification of a granulocyte colony-stimulating factor from the conditioned medium of a subclone of human bladder carcinoma cell line 5637, HTB9

1990 ◽  
Vol 190 (1) ◽  
pp. 229-238 ◽  
Author(s):  
E. Morioka ◽  
S. Taniguchi ◽  
S. Okamura ◽  
T. Shibuya ◽  
Y. Niho
Keyword(s):  
Cell Line ◽  
Conditioned Medium ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Human Bladder ◽  
Bladder Carcinoma Cell Line ◽  
Stimulating Factor

scholarly journals CULTURE OF COLONY-STIMULATING FACTOR (CSF)-PRODUCING HUMAN BLADDER CARCINOMA CELL LINE (HTB9) ON ARTIFICIAL CAPILLARIES

1987 ◽  
Vol 8 (5) ◽  
pp. 301-305 ◽  
Author(s):  
FUSAYUKI OMORI ◽  
SEIICHI OKAMURA ◽  
SHIN HAYASHI ◽  
SHIGERU YAMAGA ◽  
TSUNEFUMI SHIBUYA ◽  
...  
Keyword(s):  
Cell Line ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Colony Stimulating Factor ◽  
Human Bladder ◽  
Bladder Carcinoma Cell Line ◽  
Stimulating Factor

scholarly journals Production of leukemic blast growth factor by a human bladder carcinoma cell line

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 748-751
Author(s):  
T Hoang ◽  
EA McCulloch
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Conditioned Medium ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Leukemic Blast ◽  
Human Bladder ◽  
Complete Replacement ◽  
Bladder Carcinoma Cell Line

Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.

scholarly journals Production of leukemic blast growth factor by a human bladder carcinoma cell line

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 748-751 ◽  
Author(s):  
T Hoang ◽  
EA McCulloch
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Conditioned Medium ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Leukemic Blast ◽  
Human Bladder ◽  
Complete Replacement ◽  
Bladder Carcinoma Cell Line

Abstract Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.

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