Screening for New Metabolites from Marine Microorganisms

2005 ◽  
pp. 1-48 ◽  
Author(s):  
Thomas Schweder ◽  
Ulrike Lindequist ◽  
Michael Lalk
Keyword(s):  
Marine Microorganisms ◽  
New Metabolites

scholarly journals Secondary metabolites from marine microorganisms

2002 ◽  
Vol 74 (1) ◽  
pp. 151-170 ◽  
Author(s):  
ALPHONSE KELECOM
Keyword(s):  
Secondary Metabolites ◽  
Cyclic Peptides ◽  
Marine Invertebrates ◽  
Indole Alkaloids ◽  
Marine Sponges ◽  
Annual Reports ◽  
Marine Microorganisms ◽  
Mature Field ◽  
Bacteria And Fungi ◽  
New Metabolites

After 40 years of intensive research, chemistry of marine natural products has become a mature field. Since 1995, there are signals of decreased interest in the search of new metabolites from traditional sources such as macroalgae and octocorals, and the number of annual reports on marine sponges stabilized. On the contrary, metabolites from microorganisms is a rapidly growing field, due, at least in part, to the suspicion that a number of metabolites obtained from algae and invertebrates may be produced by associated microorganisms. Studies are concerned with bacteria and fungi, isolated from seawater, sediments, algae, fish and mainly from marine invertebrates such as sponges, mollusks, tunicates, coelenterates and crustaceans. Although it is still to early to define tendencies, it may be stated that the metabolites from microorganisms are in most cases quite different from those produced by the invertebrate hosts. Nitrogenated metabolites predominate over acetate derivatives, and terpenes are uncommon. Among the latter, sesquiterpenes, diterpenes and carotenes have been isolated; among nitrogenated metabolites, amides, cyclic peptides and indole alkaloids predominate.

scholarly journals Comparative Proteomics of Marinobacter sp. TT1 Reveals Corexit Impacts on Hydrocarbon Metabolism, Chemotactic Motility, and Biofilm Formation

2020 ◽  
Vol 9 (1) ◽  
pp. 3
Author(s):  
Saskia Rughöft ◽  
Nico Jehmlich ◽  
Tony Gutierrez ◽  
Sara Kleindienst
Keyword(s):  
Biofilm Formation ◽  
Oil Spills ◽  
Carbon Sources ◽  
Comparative Proteomics ◽  
Hydrocarbon Biodegradation ◽  
Solvent Tolerance ◽  
Marine Microorganisms ◽  
Degrading Bacteria ◽  
Oil Spill Response ◽  
First Time

The application of chemical dispersants during marine oil spills can affect the community composition and activity of marine microorganisms. Several studies have indicated that certain marine hydrocarbon-degrading bacteria, such as Marinobacter spp., can be inhibited by chemical dispersants, resulting in lower abundances and/or reduced biodegradation rates. However, a major knowledge gap exists regarding the mechanisms underlying these physiological effects. Here, we performed comparative proteomics of the Deepwater Horizon isolate Marinobacter sp. TT1 grown under different conditions. Strain TT1 received different carbon sources (pyruvate vs. n-hexadecane) with and without added dispersant (Corexit EC9500A). Additional treatments contained crude oil in the form of a water-accommodated fraction (WAF) or chemically-enhanced WAF (CEWAF; with Corexit). For the first time, we identified the proteins associated with alkane metabolism and alginate biosynthesis in strain TT1, report on its potential for aromatic hydrocarbon biodegradation and present a protein-based proposed metabolism of Corexit components as carbon substrates. Our findings revealed that Corexit exposure affects hydrocarbon metabolism, chemotactic motility, biofilm formation, and induces solvent tolerance mechanisms, like efflux pumps, in strain TT1. This study provides novel insights into dispersant impacts on microbial hydrocarbon degraders that should be taken into consideration for future oil spill response actions.

scholarly journals Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 256
Author(s):  
Yehui Gang ◽  
Tae-Yang Eom ◽  
Svini Dileepa Marasinghe ◽  
Youngdeuk Lee ◽  
Eunyoung Jo ◽  
...  
Keyword(s):  
Water Content ◽  
Antioxidant Activities ◽  
Fermented Foods ◽  
Solution Stability ◽  
Marine Microorganism ◽  
Marine Microorganisms ◽  
Dpph Assay ◽  
Aqueous Methanol ◽  
Potential Applications ◽  
Pseudoalteromonas Rubra

Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect that of the samples. Based on solution stability considerations, 70% aqueous methanol was chosen as the optimal DPPH solvent. The developed method was successfully applied to the cell-free supernatants of marine bacteria (Pseudoalteromonas rubra and Pseudoalteromonas xiamenensis), revealing their high antioxidant activities. Furthermore, it was concluded that this method would be useful for the screening of marine microorganism–derived antioxidants, which also has numerous potential applications, such as salt-fermented foods.

scholarly journals THE CHARACTERIZATION OF FOUR NEW METABOLITES OF ADRENOCORTICAL HORMONES

1955 ◽  
Vol 212 (1) ◽  
pp. 449-460 ◽  
Author(s):  
David K. Fukushima ◽  
Norma S. Leeds ◽  
H. Leon Bradlow ◽  
Theodore H. Kritchevsky ◽  
Madeleine B. Stokem ◽  
...  
Keyword(s):  
Adrenocortical Hormones ◽  
New Metabolites

scholarly journals Marine microorganisms as an untapped source of bioactive compounds

2021 ◽  
Vol 28 (1) ◽  
pp. 224-231
Author(s):  
Fuad Ameen ◽  
Saleh AlNadhari ◽  
Ali A. Al-Homaidan
Keyword(s):  
Bioactive Compounds ◽  
Marine Microorganisms

Lactarane Type Sesquiterpenoids as Inhibitors of Leukotriene Biosynthesis and Other, New Metabolites from Submerged Cultures of Lentinellus cochleatus (Pers. ex Fr.) Karst

1996 ◽  
Vol 51 (7-8) ◽  
pp. 493-499 ◽  
Author(s):  
Annette Wunder ◽  
Timm Anke ◽  
Dörte Klostermeyer ◽  
Wolfgang Steglich
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Submerged Cultures ◽  
Blood Leukocytes ◽  
Spectroscopic Investigations ◽  
Basophilic Leukemia ◽  
New Metabolites

Abstract Three known sesquiterpenoids of the lactarane and secolactarane type, deoxylactarorufin A (1), blennin A (2) and blennin C (3), have been obtained from cultures of Lentinellus cochleatus (Basidiomycetes) together with the new metabolites (Z)-2-chloro-3-(4-me-thoxyphenyl)-2-propen-l-ol (4) and lentinellone (5), a protoilludane derivative. The structures were determined by spectroscopic investigations. 1, 2 and 3 are potent inhibitors of leukotriene biosynthesis in rat basophilic leukemia (RBL-1) cells and human peripheral blood leukocytes (PBL).

New metabolites of Azotobacter vinelandii exhibiting antifungal activity

Microbiology ◽  
2009 ◽  
Vol 78 (4) ◽  
pp. 428-432 ◽  
Author(s):  
S. P. Chetverikov ◽  
O. N. Loginov
Keyword(s):  
Antifungal Activity ◽  
Azotobacter Vinelandii ◽  
New Metabolites

scholarly journals O-Demethylpaulomycins A and B, U-77,802 and U-77,803, paulomenols A and B, new metabolites produced by Streptomyces paulus.

1988 ◽  
Vol 41 (10) ◽  
pp. 1316-1330 ◽  
Author(s):  
A. D. ARGOUDELIS ◽  
L. BACZYNSKYJ ◽  
S. A. MIZSAK ◽  
F. B. SHILLIDAY
Keyword(s):  
New Metabolites

Cytotoxic effects of three new metabolites from Red Sea marine sponge, Petrosia sp.

2014 ◽  
Vol 37 (3) ◽  
pp. 928-935 ◽  
Author(s):  
Ahmed Abdel-Lateff ◽  
Walied M. Alarif ◽  
Hany Z. Asfour ◽  
Seif-Eldin N. Ayyad ◽  
Alaa Khedr ◽  
...  
Keyword(s):  
Red Sea ◽  
Marine Sponge ◽  
Cytotoxic Effects ◽  
New Metabolites
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