The immobilisation of DNA strands on silica surface by means of chemical grafting

2004 ◽  
pp. 275-279 ◽  
Author(s):  
V. Dugas ◽  
Y. Chevalier ◽  
G. Depret ◽  
X. Nesme ◽  
É. Souteyr
Author(s):  
V. Dugas ◽  
Y. Chevalier ◽  
G. Depret ◽  
X. Nesme ◽  
É. Souteyr

2005 ◽  
Vol 23 (9) ◽  
pp. 703-719 ◽  
Author(s):  
L.A. Belyakova ◽  
A.M. Varvarin ◽  
D.Yu. Lyashenko ◽  
O.V. Khora

The chemical interaction between the surface of hydroxylated or aminated silicas and β-cyclodextrin, mono-tosyl-β-cyclodextrin and the bromine derivative of heptakis-[6- O-( p-tosyl-β-cyclodextrin)] has been investigated using IR spectroscopy, thermogravimetric analysis with programmed heating, pH titration, weight adsorption method, elemental analysis and quantitative chemical analysis of the surface compounds. The optimum conditions for chemical grafting of β-cyclodextrins onto the silica surface were established. The formation of supramolecular structures, viz. inclusion complexes between immobilized β-cyclodextrin and the hormone of pineal gland (melatonin), on the surface of highly dispersed silicas of 1:1 composition was demonstrated.


2013 ◽  
Vol 717 ◽  
pp. 90-94
Author(s):  
Byung Hun Chang ◽  
Se Hee Lee ◽  
Anantha Iyengar Gopalan ◽  
Kwang Pill Lee

In this report, the chains of poly (aminophenylbornoic acid) (PAPBA) were grafted onto the surface of silica nanoparticles to obtain Si-g-PAPBA. Si-g-PAPBA was characterized through field emission scanning electron microscopy (FESEM), fourier transform infrared (FTIR) spectroscopy, UV-Vis spectroscopy and thermogravimetric analysis (TGA). Grafting of PAPBA chains onto silica surface was confirmed by TGA and FTIR spectroscopy.


Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.


Author(s):  
S.K. Aggarwal ◽  
J.M. Fadool

Cisplatin (CDDP) a potent antitumor agent suffers from severe toxic side effects with nephrotoxicity being the major dose-limiting factor, The primary mechanism of its action has been proposed to be through its cross-linking DNA strands. It has also been shown to inactivate various transport enzymes and induce hypocalcemia and hypomagnesemia that may be the underlying cause for some of its toxicities. The present is an effort to study its influence on the parathyroid gland for any hormonal changes that control calcium levels in the body.Male Swiss Wistar rats (Crl: (WI) BR) weighing 200-300 g and of 60 days in age were injected (ip) with cisplatin (7mg/kg in normal saline). The controls received saline injections only. The animals were injected (iv) with calcium (0.5 ml of 10% calcium gluconate/day) and were killed by decapitation on day 1 through 5. Trunk blood was collected in heparinized tubes.


2003 ◽  
Vol 772 ◽  
Author(s):  
T. Seeger ◽  
G. de la Fuente ◽  
W.K. Maser ◽  
A.M. Benito ◽  
A. Righi ◽  
...  

AbstractCarbon nanotubes (CNT) are interesting candidates for the reinforcement in robust composites and for conducting fillers in polymers due to their fascinating electronic and mechanical properties. For the first time, we report the incorporation of multi walled carbon nanotubes (MWNTs) into silica-glass surfaces by means of partial surface-melting caused by a continuous wave Nd:YAG laser. MWNTs were detected being well incorporated in the silica-surface. The composites are characterized using scanning electron microscopy (SEM) and Raman-spectroscopy. A model for the composite-formation is proposed based on heatabsorption by MWNTs and a partial melting of the silica-surface.


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