scholarly journals Heterologous Expression in Yeast of Human Ornithine Carriers ORNT1 and ORNT2 and of ORNT1 Alleles Implicated in HHH Syndrome in Humans

2015 ◽  
pp. 119-126 ◽  
Author(s):  
Mara Doimo ◽  
Raffaele Lopreiato ◽  
Valentina Basso ◽  
Raissa Bortolotto ◽  
Alessandra Tessa ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Heterologous Expression In Yeast

Contribution of human cytochrome P450 to benzo[a]pyrene and benzo[a]pyrene-7,8-dihydrodiol metabolism, as predicted from heterologous expression in yeast

1996 ◽  
Vol 6 (6) ◽  
pp. 489-499 ◽  
Author(s):  
Jean-Charles Gautier ◽  
Sylvaine Lecoeur ◽  
Jos?? Cosme ◽  
Alain Perret ◽  
Philippe Prban ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Heterologous Expression ◽  
Human Cytochrome ◽  
Heterologous Expression In Yeast

scholarly journals Validation of a Candidate Deoxynivalenol-Inactivating UDP-Glucosyltransferase from Barley by Heterologous Expression in Yeast

2010 ◽  
Vol 23 (7) ◽  
pp. 977-986 ◽  
Author(s):  
Wolfgang Schweiger ◽  
Jayanand Boddu ◽  
Sanghyun Shin ◽  
Brigitte Poppenberger ◽  
Franz Berthiller ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Sequence Similarity ◽  
Gene Products ◽  
Fusarium Spp ◽  
High Sequence Similarity ◽  
Detoxification Enzyme ◽  
Large Gene ◽  
Yeast Transformants ◽  
Very High ◽  
Heterologous Expression In Yeast

Resistance to the virulence factor deoxynivalenol (DON) due to formation of DON-3-O-glucoside (D3G) is considered to be an important component of resistance against Fusarium spp. which produce this toxin. Multiple candidate UDP-glycosyltransferase (UGT) genes from different crop plants that are either induced by Fusarium spp. or differentially expressed in cultivars varying in Fusarium disease resistance have been described. However, UGT are encoded by a very large gene family in plants. The study of candidate plant UGT is highly warranted because of the potential relevance for developing Fusarium-spp.-resistant crops. We tested Arabidopsis thaliana genes closely related to a previously identified DON-glucosyltransferase gene by heterologous expression in yeast and showed that gene products with very high sequence similarity can have pronounced differences in detoxification capabilities. We also tested four candidate barley glucosyltransferases, which are highly DON inducible. Upon heterologous expression of full-length cDNAs, only one gene, HvUGT13248, conferred DON resistance. The conjugate D3G accumulated in the supernatant of DON-treated yeast transformants. We also present evidence that the product of the TaUGT3 gene recently proposed to encode a DON-detoxification enzyme of wheat does not protect yeast against DON.

scholarly journals Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast

FEBS Letters ◽  
1994 ◽  
Vol 351 (2) ◽  
pp. 286-290 ◽  
Author(s):  
François Périer ◽  
Kathryn L. Coulter ◽  
Hong Liang ◽  
Carolyn M. Radeke ◽  
Richard F. Gaber ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Heterologous Expression In Yeast

scholarly journals Mutagenesis and heterologous expression in yeast of a plant Δ6‐fatty acid desaturase

2001 ◽  
Vol 52 (360) ◽  
pp. 1581-1585 ◽  
Author(s):  
Olga Sayanova ◽  
Frédéric Beaudoin ◽  
Balázs Libisch ◽  
Aude Castel ◽  
Peter R. Shewry ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Heterologous Expression ◽  
Fatty Acid Desaturase ◽  
Heterologous Expression In Yeast

Cloning and molecular characterization of the Δ6-desaturase from two Echium plant species: Production of GLA by heterologous expression in yeast and tobacco

Lipids ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 417-426 ◽  
Author(s):  
Federico García-Maroto ◽  
José A. Garrido-Cárdénas ◽  
Juan Rodríguez-Ruiz ◽  
Miguel Vilches-Ferrón ◽  
Ana C. Adam ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Plant Species ◽  
Species Production ◽  
Heterologous Expression In Yeast ◽  
Δ6 Desaturase
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