Protocols for Investigating the Microbial Communities of Oil and Gas Reservoirs

2016 ◽  
pp. 65-109 ◽  
Author(s):  
Nicolas Tsesmetzis ◽  
Michael J. Maguire ◽  
Ian M. Head ◽  
Bart P. Lomans
Keyword(s):  
Microbial Communities ◽  
Oil And Gas ◽  
Gas Reservoirs ◽  
Oil And Gas Reservoirs

scholarly journals Diversity and abundance of <i>n</i>-alkane-degrading bacteria in the near-surface soils of a Chinese onshore oil and gas field

2013 ◽  
Vol 10 (3) ◽  
pp. 2041-2048 ◽  
Author(s):  
K. Xu ◽  
Y. Tang ◽  
C. Ren ◽  
K. Zhao ◽  
Y. Sun
Keyword(s):  
Microbial Communities ◽  
Oil And Gas ◽  
Gas Reservoirs ◽  
Surface Soils ◽  
Gas Field ◽  
Near Surface ◽  
Degrading Bacteria ◽  
Oil And Gas Field ◽  
Oil And Gas Reservoirs ◽  
Alkb Genes

Abstract. Alkane-degrading bacteria have long been used as an important biological indicator for oil and gas prospecting, but their ecological characteristics in hydrocarbon microseep habitats are still poorly understood. In this study, the diversity and abundance of n-alkane-degrading bacterial community in the near-surface soils of a Chinese onshore oil and gas field were investigated using molecular techniques. Terminal restriction fragment length polymorphism (T-RFLP) analyses in combination with cloning and sequencing of alkB genes revealed that Gram-negative genotypes (Alcanivorax and Acinetobacter) dominated n-alkane-degrading bacterial communities in the near-surface soils of oil and gas reservoirs, while the dominant microbial communities were Gram-positive bacteria (Mycobacterium and Rhodococcus) in background soil. Real-time quantitative polymerase chain reaction (PCR) results furthermore showed that the abundance of alkB genes increased substantially in the surface soils above oil and gas reservoirs even though only low or undetectable concentrations of hydrocarbons were measured in these soils. The results of this study implicate that trace amounts of volatile hydrocarbons migrate from oil and gas reservoirs, and likely result in the changes of microbial communities in the near-surface soil.

FRACTURE MODELING IN OIL AND GAS RESERVOIRS USING IMAGE LOGS DATA AND PETREL SOFTWARE

2015 ◽  
Vol 75 (11) ◽  
Author(s):  
Mostafa Alizadeh ◽  
Zohreh Movahed ◽  
Radzuan Junin ◽  
Rahmat Mohsin ◽  
Mehdi Alizadeh ◽  
...  
Keyword(s):  
Oil And Gas ◽  
Fracture Permeability ◽  
Gas Reservoirs ◽  
New Information ◽  
Fracture Modeling ◽  
Fracture Intensity ◽  
Major Fault ◽  
Oil And Gas Reservoirs ◽  
Minimum Stress ◽  
Longitudinal Fractures

The purpose of modelling the fractures is to create simulation properties with the power to predict the reservoir behaviour. Petrel software is one of the best softwares in the market that can do this task very well, but there is no available educational paper for every researcher. Therefore, in this work, a fracture modelling job was done in one of the most important Iranian fields using Petrel software and image log data. The purpose of this work was  to determine the new information of the fractures in Gachsaran field and also to prepare a valuable educational paper for other researchers who are interested to learn about the fracture modelling. This work revealed that in this field, the longitudinal fractures had been parallel to minimum stress (Zagros trend), fracture intensity was the nearest to the major fault and northern flank, fracture porosity was 0-7%, fracture permeability was 0-6000 MD, and more valuable information is provided in this paper.

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