Single-Cell Cultivation in Microfluidic Devices

2015 ◽  
pp. 5-16 ◽  
Author(s):  
Sattar Taheri-Araghi ◽  
Suckjoon Jun
Keyword(s):  
Single Cell ◽  
Microfluidic Devices ◽  
Cell Cultivation

Single-Cell Cultivation Utilizing Microfluidic Systems

2020 ◽  
pp. 1-24
Author(s):  
Dian Anggraini ◽  
Nobutoshi Ota ◽  
Yigang Shen ◽  
Yo Tanaka ◽  
Yoichiroh Hosokawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Cultivation ◽  
Microfluidic Systems

scholarly journals A diffusion-based microfluidic device for single-cell RNA-seq

Lab on a Chip ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1247-1256 ◽  
Author(s):  
Mimosa Sarma ◽  
Jiyoung Lee ◽  
Sai Ma ◽  
Song Li ◽  
Chang Lu
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Microfluidic Devices ◽  
Rna Seq ◽  
Transcriptomic Studies

Scalable microfluidic devices containing reaction and loading chambers were developed to conduct single-cell transcriptomic studies.

scholarly journals Single-cell Analysis with Microfluidic Devices

2019 ◽  
Vol 35 (6) ◽  
pp. 609-618 ◽  
Author(s):  
Xiaowen OU ◽  
Peng CHEN ◽  
Bi-Feng LIU
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Microfluidic Devices ◽  
Cell Analysis

scholarly journals New Approaches for Isolation of Previously Uncultivated Oral Bacteria

2011 ◽  
Vol 78 (1) ◽  
pp. 194-203 ◽  
Author(s):  
M. V. Sizova ◽  
T. Hohmann ◽  
A. Hazen ◽  
B. J. Paster ◽  
S. R. Halem ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Single Cell ◽  
Single Species ◽  
Oral Bacteria ◽  
Cell Cultivation ◽  
Human Oral Cavity ◽  
Successful Recovery ◽  
Number Of Microorganisms ◽  
Enrichment Techniques

ABSTRACTA significant number of microorganisms from the human oral cavity remain uncultivated. This is a major impediment to the study of human health since some of the uncultivated species may be involved in a variety of systemic diseases. We used a range of innovations previously developed to cultivate microorganisms from the human oral cavity, focusing on anaerobic species. These innovations include (i)in vivocultivation to specifically enrich for species actively growing in the oral cavity (the “minitrap” method), (ii) single-cell long-term cultivation to minimize the effect of fast-growing microorganisms, and (iii) modifications of conventional enrichment techniques, using media that did not contain sugar, including glucose. To enable cultivation of obligate anaerobes, we maintained strict anaerobic conditions in most of our cultivation experiments. We report that, on a per cell basis, the most successful recovery was achieved using minitrap enrichment (11%), followed by single-cell cultivation (3%) and conventional plating (1%). Taxonomically, the richest collection was obtained using the single-cell cultivation method, followed by minitrap and conventional enrichment, comprising representatives of 13, 9, and 4 genera, respectively. Interestingly, no single species was isolated by all three methods, indicating method complementarity. An important result is the isolation and maintenance in pure culture of 10 strains previously only known by their molecular signatures, as well as representatives of what are likely to be three new microbial genera. We conclude that the ensemble of new methods we introduced will likely help close the gap between cultivated and uncultivated species from the human oral cavity.

Measurement of Incident Angle Dependence of Swimming Bacterium Reflection Using On-Chip Single-Cell Cultivation Assay

2003 ◽  
Vol 42 (Part 2, No. 7B) ◽  
pp. L873-L875 ◽  
Author(s):  
Akihiro Hattori ◽  
Senkei Umehara ◽  
Yuichi Wakamoto ◽  
Kenji Yasuda
Keyword(s):  
Single Cell ◽  
Incident Angle ◽  
Cell Cultivation ◽  
Angle Dependence ◽  
On Chip
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