Flow Cytometric Determination of Microbial Abundances and Its Use to Obtain Indices of Community Structure and Relative Activity

2015 ◽  
pp. 159-187 ◽  
Author(s):  
Josep M. Gasol ◽  
Xosé Anxelu G. Morán
Keyword(s):  
Community Structure ◽  
Relative Activity ◽  
Flow Cytometric

scholarly journals Flow Cytometric Determination of Genome Size in the Taxodiaceae, Cupressaceae sensu stricto and Sciadopityaceae.

CYTOLOGIA ◽  
2001 ◽  
Vol 66 (3) ◽  
pp. 307-311 ◽  
Author(s):  
Masahiro Hizume ◽  
Teiji Kondo ◽  
Fukashi Shibata ◽  
Ryoko Ishizuka
Keyword(s):  
Genome Size ◽  
Sensu Stricto ◽  
Flow Cytometric

scholarly journals OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS

2014 ◽  
Vol 9 (3) ◽  
pp. 97
Author(s):  
Ninoek Indriati ◽  
Arifah Kusmarwati ◽  
Irma Hermana
Keyword(s):  
Lactococcus Lactis ◽  
Growth Curve ◽  
Growth Conditions ◽  
Relative Activity ◽  
Bacteriocin Production ◽  
Bacteriocin Activity ◽  
Carbohydrate Source ◽  
Fermented Fish ◽  
Producer Cell

Previous study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a  isolated from rusip, a traditional Bangkanese fermented fish product.The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2L). Followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity on that scale, and also stability of bacteriocin during storage.The results showed that the optimum temperature and pH for the growth of producer cell were 28°C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH2PO4, 0,2% NaCl and 0,02% MgSO4.7H20) in addition of sucrose as carbohydrate source. Based on the growth curve performedon CM medium with KH2PO4, the L. Lactis ssp lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was  8000 AU/mlat24th hour.Bacteriocin  was relatively stable when stored at -20°C for 1month with a relative activity of 69,4%.

scholarly journals Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease

Cytometry ◽  
1992 ◽  
Vol 13 (8) ◽  
pp. 893-901 ◽  
Author(s):  
Johannes Drach ◽  
Doris Drach ◽  
Herta Glassl ◽  
Claus Gattringer ◽  
Heinz Huber
Keyword(s):  
Acute Leukemia ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Antigen Expression ◽  
Flow Cytometric ◽  
Minimal Residual

scholarly journals Flow cytometric determination of glutathione in clinical samples

Cytometry ◽  
1995 ◽  
Vol 21 (1) ◽  
pp. 68-71 ◽  
Author(s):  
Sue Chow ◽  
David Hedley
Keyword(s):  
Clinical Samples ◽  
Flow Cytometric
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