ActA of Listeria monocytogenes and Its Manifold Activities as an Important Listerial Virulence Factor

2016 ◽  
pp. 113-132 ◽  
Author(s):  
Helena Pillich ◽  
Madhu Puri ◽  
Trinad Chakraborty
Keyword(s):  
Listeria Monocytogenes ◽  
Virulence Factor

scholarly journals Fatty Acids Regulate Stress Resistance and Virulence Factor Production for Listeria monocytogenes

2012 ◽  
Vol 194 (19) ◽  
pp. 5274-5284 ◽  
Author(s):  
Y. Sun ◽  
B. J. Wilkinson ◽  
T. J. Standiford ◽  
H. T. Akinbi ◽  
M. X. D. O'Riordan
Keyword(s):  
Fatty Acids ◽  
Listeria Monocytogenes ◽  
Virulence Factor ◽  
Stress Resistance ◽  
Factor Production ◽  
Virulence Factor Production

scholarly journals The inlA Gene of Listeria monocytogenesLO28 Harbors a Nonsense Mutation Resulting in Release of Internalin

1998 ◽  
Vol 66 (7) ◽  
pp. 3420-3422 ◽  
Author(s):  
Renaud Jonquières ◽  
Hélène Bierne ◽  
Jérôme Mengaud ◽  
Pascale Cossart
Keyword(s):  
Cell Adhesion ◽  
Listeria Monocytogenes ◽  
Virulence Factor ◽  
Adhesion Molecule ◽  
Nonsense Mutation ◽  
Cell Adhesion Molecule ◽  
Culture Medium ◽  
Surface Protein ◽  
Truncated Protein

ABSTRACT Internalin is a surface protein that mediates entry ofListeria monocytogenes EGD into epithelial cells expressing the cell adhesion molecule human E-cadherin or its chicken homolog, L-CAM, which act as receptors for internalin. After observing that entry of L. monocytogenes LO28 into S180 fibroblasts, in contrast to that of EGD, did not increase after transfection with L-CAM, we examined both the expression and the structure of internalin in strain LO28. We discovered a nonsense mutation in inlA which results in a truncated protein released in the culture medium. Mutations leading to release of internalin were also detected in clinical and food isolates. These results question the role of internalin as a virulence factor in murine listeriosis.

Listeria monocytogenes bile salt hydrolase is a virulence factor involved in the intestinal and hepatic phases of listeriosis

2002 ◽  
Vol 46 (3) ◽  
pp. 903-903 ◽  
Author(s):  
Olivier Dussurget ◽  
Didier Cabanes ◽  
Pierre Dehoux ◽  
Marc Lecuit ◽  
Carmen Buchrieser ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Bile Salt ◽  
Virulence Factor ◽  
Bile Salt Hydrolase

Virulence of Listeria monocytogenes Propagated in NaCl Containing Media at 4, 25 and 37°C

1994 ◽  
Vol 57 (6) ◽  
pp. 475-478 ◽  
Author(s):  
ERIC R. MYERS ◽  
SCOTT E. MARTIN
Keyword(s):  
Superoxide Dismutase ◽  
Listeria Monocytogenes ◽  
Sodium Chloride ◽  
Mouse Model ◽  
Virulence Factor ◽  
Growth Temperature ◽  
Lethal Dose ◽  
Listeriolysin O ◽  
Sod Activity ◽  
Approximate Lethal Dose

Virulence, as determined in a mouse model, and virulence factor activities of catalase (CA), superoxide dismutase (SOD) and listeriolysin O (LLO), was examined in Listeria monocytogenes 10403S. Cells were propagated in media containing various concentrations of sodium chloride (NaCl) at 4, 25 and 37°C. Strain 10403S exhibited significant increases in CA activity and LLO when grown in media containing 428 mM of NaCl at 37°C. The CA activities at 4 and 25°C were significantly less, and the cells exhibited similar increases and decreases as cells grown at 37°C. When comparing the growth temperatures, the CA activity decreased as the growth temperature decreased. The SOD activity was significantly increased only when cells were propagated in media containing either 428 or 1,112 mM of NaCl. The SOD activity increased as the growth temperature decreased. No LLO activity was detected when cells were grown at 4 and 25°C. The production of these enzymes appeared to be thermoregulated. In addition, approximate lethal dose (ALD50) values were determined after intragastric (i.g.) and intraperitoneal (i.p.) infection. Each method of infection indicated that LLO was required for virulence, while growth in salt containing media, growth at 4°C, or the production of higher levels of CA, SOD and LLO did not appear to influence the virulence of L. monocytogenes.

FbpA, a novel multifunctional Listeria monocytogenes virulence factor

2004 ◽  
Vol 53 (2) ◽  
pp. 639-649 ◽  
Author(s):  
S. Dramsi ◽  
F. Bourdichon ◽  
D. Cabanes ◽  
M. Lecuit ◽  
H. Fsihi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Virulence Factor

scholarly journals Bacteriocin from epidemic Listeria strains alters the host intestinal microbiota to favor infection

2016 ◽  
Vol 113 (20) ◽  
pp. 5706-5711 ◽  
Author(s):  
Juan J. Quereda ◽  
Olivier Dussurget ◽  
Marie-Anne Nahori ◽  
Amine Ghozlane ◽  
Stevenn Volant ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Intestinal Microbiota ◽  
Virulence Factor ◽  
Invasive Disease ◽  
Immunocompromised Patients ◽  
Intestinal Colonization ◽  
Healthy Individuals ◽  
Virulence Potential ◽  
Evolutionary Lineages ◽  
Organ Infection

Listeria monocytogenes is responsible for gastroenteritis in healthy individuals and for a severe invasive disease in immunocompromised patients. Among the three identified L. monocytogenes evolutionary lineages, lineage I strains are overrepresented in epidemic listeriosis outbreaks, but the mechanisms underlying the higher virulence potential of strains of this lineage remain elusive. Here, we demonstrate that Listeriolysin S (LLS), a virulence factor only present in a subset of lineage I strains, is a bacteriocin highly expressed in the intestine of orally infected mice that alters the host intestinal microbiota and promotes intestinal colonization by L. monocytogenes, as well as deeper organ infection. To our knowledge, these results therefore identify LLS as the first bacteriocin described in L. monocytogenes and associate modulation of host microbiota by L. monocytogenes epidemic strains to increased virulence.

scholarly journals Bacteriocins of Listeria monocytogenes and Their Potential as a Virulence Factor

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 103
Author(s):  
Sangmi Lee
Keyword(s):  
Listeria Monocytogenes ◽  
Virulence Factors ◽  
Intestinal Microbiota ◽  
Virulence Factor ◽  
Mode Of Action ◽  
Foodborne Pathogen ◽  
Protective Effects ◽  
Fatality Rate ◽  
Factors Affecting ◽  
High Fatality Rate

Intestinal microbiota exerts protective effects against the infection of various bacterial pathogens, including Listeria monocytogenes, a major foodborne pathogen whose infection can lead to a disease (listeriosis) with a high fatality rate. As a strategy to mitigate the action of the intestinal microbiota, pathogens often produce antimicrobial proteinaceous compounds such as bacteriocins. In this review, we summarize the information currently available for the well-characterized L. monocytogenes bacteriocin listeriolysin S, with the emphasis on its intriguing mode of action as a virulence factor, which promotes the infection of L. monocytogenes by changing the composition of the intestinal microbiota. We then discuss another intriguing L. monocytogenes bacteriocin Lmo2776 that specifically inhibits the inflammogenic species, Prevotella copri, in the intestinal microbiota, reducing superfluous inflammation while weakening virulence. In addition, we describe relatively less studied phage tail-like Listeria bacteriocins (monocins) and elaborate on the possibility that these monocins could be involved in enhancing pathogenicity. In spite of the burgeoning interest in the roles played by the intestinal microbiota against the L. monocytogenes infection, our understanding on the virulence factors affecting the intestinal microbiota is still lacking, calling for further studies on bacteriocins that could function as novel virulence factors.

scholarly journals Secretion Chaperones PrsA2 and HtrA Are Required for Listeria monocytogenes Replication following Intracellular Induction of Virulence Factor Secretion

2016 ◽  
Vol 84 (10) ◽  
pp. 3034-3046 ◽  
Author(s):  
Jana K. Ahmed ◽  
Nancy E. Freitag
Keyword(s):  
Listeria Monocytogenes ◽  
Virulence Factor ◽  
Bacterial Growth ◽  
Cell Types ◽  
Broth Culture ◽  
Bacterial Invasion ◽  
Bacterial Membrane ◽  
Content Type ◽  
Factor Secretion

The Gram-positive bacteriumListeria monocytogenestransitions from an environmental organism to an intracellular pathogen following its ingestion by susceptible mammalian hosts. Bacterial replication within the cytosol of infected cells requires activation of the central virulence regulator PrfA followed by a PrfA-dependent induction of secreted virulence factors. The PrfA-induced secreted chaperone PrsA2 and the chaperone/protease HtrA contribute to the folding and stability of select proteins translocated across the bacterial membrane.L. monocytogenesstrains that lack bothprsA2andhtrAexhibit near-normal patterns of growth in broth culture but are severely attenuatedin vivo. We hypothesized that, in the absence of PrsA2 and HtrA, the increase in PrfA-dependent protein secretion that occurs following bacterial entry into the cytosol results in misfolded proteins accumulating at the bacterial membrane with a subsequent reduction in intracellular bacterial viability. Consistent with this hypothesis, the introduction of a constitutively activated allele ofprfA(prfA*) into ΔprsA2ΔhtrAstrains was found to essentially inhibit bacterial growth at 37°C in broth culture. ΔprsA2ΔhtrAstrains were additionally found to be defective for cell invasion and vacuole escape in selected cell types, steps that precede full PrfA activation. These data establish the essential requirement for PrsA2 and HtrA in maintaining bacterial growth under conditions of PrfA activation. In addition, chaperone function is required for efficient bacterial invasion and rapid vacuole lysis within select host cell types, indicating roles for PrsA2/HtrA prior to cytosolic PrfA activation and the subsequent induction of virulence factor secretion.

Assessment of Environmental Factors on Listeria monocytogenes Scott A inlA Gene Expression by Relative Quantitative Taqman Real-Time Reverse Transcriptase PCR

2006 ◽  
Vol 69 (11) ◽  
pp. 2754-2757 ◽  
Author(s):  
SCOTT E. HANNA ◽  
HUA H. WANG
Keyword(s):  
Gene Expression ◽  
Listeria Monocytogenes ◽  
Environmental Factors ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Virulence Factors ◽  
Virulence Factor ◽  
Internal Standard ◽  
Reverse Transcriptase Pcr ◽  
The Impact

Several virulence factors are involved in Listeria monocytogenes pathogenicity. L. monocytogenes internalins, particularly internalin A, are required for bacterial adhesion to and invasion of human intestinal epithelial cells. The expression of inter-nalins is thus related to virulence. Identification of conditions involved in regulating the expression of L. monocytogenes virulence factors is essential for developing targeted strategies to control listeriosis incidence and improving therapeutic approaches. The primary aim of this study was to develop a quantitative real-time reverse transcriptase PCR platform to study the impact of environmental factors on L. monocytogenes Scott A virulence factor expression, particularly in potentially complex ecosystems. A Taqman PCR–based, rapid quantitative gene expression evaluation method was established with the L. monocytogenes ribosomal protein L4 encoding gene used as an internal standard. Our data suggest that inlA expression is influenced by food composition and temperature, indicating that certain food processing or storage conditions, such as the use of lactic and acetic acids at common storage temperatures, could affect the expression of L. monocytogenes virulence factor.

Sign in / Sign up

Export Citation Format

Share Document