The Assay of Enzyme Activity by Positron Emission Tomography

2012 ◽  
pp. 111-135 ◽  
Author(s):  
Paul Cumming ◽  
Neil Vasdev
Keyword(s):  
Positron Emission Tomography ◽  
Enzyme Activity ◽  
Emission Tomography ◽  
Positron Emission

scholarly journals [13C6,D8]2-deoxyglucose phosphorylation by hexokinase shows selectivity for the β-anomer

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gal Sapir ◽  
Talia Harris ◽  
Sivaranjan Uppala ◽  
Atara Nardi-Schreiber ◽  
Jacob Sosna ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Enzyme Activity ◽  
Molecular Imaging ◽  
Magnetic Resonance ◽  
Cancer Detection ◽  
Emission Tomography ◽  
Current Finding ◽  
Positron Emission ◽  
Anomeric Specificity ◽  
Yeast Hexokinase

AbstractA non-radioactive 2-deoxyglucose (2DG) analog has been developed here for hyperpolarized magnetic resonance investigations. The analog, [13C6,D8]2DG, showed 13% polarization in solution (27,000-fold signal enhancement at the C1 site), following a dissolution-DNP hyperpolarization process. The phosphorylation of this analog by yeast hexokinase (yHK) was monitored in real-time with a temporal resolution of 1 s. We show that yHK selectively utilizes the β anomer of the 2DG analog, thus revealing a surprising anomeric specificity of this reaction. Such anomeric selectivity was not observed for the reaction of yHK or bacterial glucokinase with a hyperpolarized glucose analog. yHK is highly similar to the human HK-2, which is overexpressed in malignancy. Thus, the current finding may shed a new light on a fundamental enzyme activity which is utilized in the most widespread molecular imaging technology for cancer detection – positron-emission tomography with 18F-2DG.

Positron Emission Tomography of Cocaine Binding Sites on the Dopamine Transporter

1994 ◽  
Author(s):  
Bertha K. Madras ◽  
◽  
David R. Elmaleh ◽  
Peter C. Meltzer ◽  
Anna Y. Liung ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Dopamine Transporter ◽  
Binding Sites ◽  
Emission Tomography ◽  
Positron Emission
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