Integrin Targeting Using RGD-Based Peptide Amphiphiles

2015 ◽  
pp. 135-155
Author(s):  
Poonam Saraf ◽  
Xiaoling Li ◽  
Bhaskara Jasti
Keyword(s):  
Peptide Amphiphiles

scholarly journals Acylation of SC4 dodecapeptide increases bactericidal potency against Gram-positive bacteria, including drug-resistant strains

2004 ◽  
Vol 378 (1) ◽  
pp. 93-103 ◽  
Author(s):  
Nathan A. LOCKWOOD ◽  
Judith R. HASEMAN ◽  
Matthew V. TIRRELL ◽  
Kevin H. MAYO
Keyword(s):  
Fatty Acid ◽  
Fluorescence Spectroscopy ◽  
Bactericidal Activity ◽  
Bacterial Cell ◽  
Membrane Surface ◽  
Peptide Amphiphiles ◽  
Helical Conformation ◽  
Gram Positive ◽  
Bacterial Membranes ◽  
Bactericidal Activities

We have conjugated dodecyl and octadecyl fatty acids to the N-terminus of SC4, a potently bactericidal, helix-forming peptide 12-mer (KLFKRHLKWKII), and examined the bactericidal activities of the resultant SC4 ‘peptide-amphiphile’ molecules. SC4 peptide-amphiphiles showed up to a 30-fold increase in bactericidal activity against Gram-positive strains (Staphylococcus aureus, Streptococcus pyogenes and Bacillus anthracis), including S. aureus strains resistant to conventional antibiotics, but little or no increase in bactericidal activity against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Fatty acid conjugation improved endotoxin (lipopolysaccharide) neutralization by 3- to 6-fold. Although acylation somewhat increased lysis of human erythrocytes, it did not increase lysis of endothelial cells, and the haemolytic effects occurred at concentrations 10- to 100-fold higher than those required for bacterial cell lysis. For insight into the mechanism of action of SC4 peptide-amphiphiles, CD, NMR and fluorescence spectroscopy studies were performed in micelle and liposome models of eukaryotic and bacterial cell membranes. CD indicated that SC4 peptide-amphiphiles had the strongest helical tendencies in liposomes mimicking bacterial membranes, and strong membrane integration of the SC4 peptide-amphiphiles was observed using tryptophan fluorescence spectroscopy under these conditions; results that correlated with the increased bactericidal activities of SC4 peptide-amphiphiles. NMR structural analysis in micelles demonstrated that the two-thirds of the peptide closest to the fatty acid tail exhibited a helical conformation, with the positively-charged side of the amphipathic helix interacting more with the model membrane surface. These results indicate that conjugation of a fatty acid chain to the SC4 peptide enhances membrane interactions, stabilizes helical structure in the membrane-bound state and increases bactericidal potency.

Exfoliated sheets of MoS2 trigger formation of aqueous gels with acute NIR light responsiveness

2016 ◽  
Vol 52 (97) ◽  
pp. 14043-14046 ◽  
Author(s):  
Ashmeet Singh ◽  
Nidhi Kapil ◽  
Mahesh Yenuganti ◽  
Dibyendu Das
Keyword(s):  
Peptide Amphiphiles ◽  
Cross Linking ◽  
Nir Light ◽  
Aqueous Gels

We have developed a unique class of MoS2 entrapped aqueous gels where the exfoliated sheets trigger physical cross-linking of nanofibers of peptide amphiphiles.

Characterization of cell selectivity, physiological stability and endotoxin neutralization capabilities of α-helix-based peptide amphiphiles

Biomaterials ◽  
2015 ◽  
Vol 52 ◽  
pp. 517-530 ◽  
Author(s):  
Zhi Ma ◽  
Dandan Wei ◽  
Ping Yan ◽  
Xin Zhu ◽  
Anshan Shan ◽  
...  
Keyword(s):  
Peptide Amphiphiles ◽  
Cell Selectivity ◽  
Α Helix

scholarly journals Creating a stem cell niche in the inner ear using self-assembling peptide amphiphiles

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0190150 ◽  
Author(s):  
Akihiro J. Matsuoka ◽  
Zafar A. Sayed ◽  
Nicholas Stephanopoulos ◽  
Eric J. Berns ◽  
Anil R. Wadhwani ◽  
...  
Keyword(s):  
Stem Cell ◽  
Inner Ear ◽  
Stem Cell Niche ◽  
Peptide Amphiphiles ◽  
Self Assembling ◽  
Cell Niche

scholarly journals Coassembled Cytotoxic and Pegylated Peptide Amphiphiles Form Filamentous Nanostructures with Potent Antitumor Activity in Models of Breast Cancer

ACS Nano ◽  
2012 ◽  
Vol 6 (9) ◽  
pp. 7956-7965 ◽  
Author(s):  
Daniel J. Toft ◽  
Tyson J. Moyer ◽  
Stephany M. Standley ◽  
Yves Ruff ◽  
Andrey Ugolkov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Peptide Amphiphiles

Peptide Amphiphiles with Distinct Supramolecular Nanostructures for Controlled Antibacterial Activities

2018 ◽  
Vol 1 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Ruilian Qi ◽  
Pengbo Zhang ◽  
Jian Liu ◽  
Lingyun Zhou ◽  
Chengcheng Zhou ◽  
...  
Keyword(s):  
Antibacterial Activities ◽  
Peptide Amphiphiles
Sign in / Sign up

Export Citation Format

Share Document