Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor

2021 ◽  
Author(s):  
Huanye Xu ◽  
Zhongxiao Cong ◽  
Yuanyuan Zhang ◽  
Wei Liu ◽  
Xiaojun Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Large Scale ◽  
Scale Expansion

Large scale manufacturing strategy for production of umbilical cord-derived mesenchymal stem cells in support of clinical trials

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S166
Author(s):  
E. Linetsky ◽  
G. Lanzoni ◽  
X. Wang ◽  
C. Lenero ◽  
A. Patel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Clinical Trials ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Large Scale ◽  
Manufacturing Strategy

Rapid Large-Scale Expansion of Functional Mesenchymal Stem Cells from Unmanipulated Bone Marrow Without Animal Serum

2008 ◽  
Vol 14 (3) ◽  
pp. 185-196 ◽  
Author(s):  
Katharina Schallmoser ◽  
Eva Rohde ◽  
Andreas Reinisch ◽  
Christina Bartmann ◽  
Daniela Thaler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Scale Expansion

scholarly journals Large-Scale Expansion of Human Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Muhammad Najib Fathi Bin Hassan ◽  
Muhammad Dain Yazid ◽  
Mohd Heikal Mohd Yunus ◽  
Shiplu Roy Chowdhury ◽  
Yogeswaran Lokanathan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Human Mesenchymal Stem Cells ◽  
Expansion Ratio ◽  
Spinner Flask ◽  
Roller Bottle ◽  
Multipotent Stem Cells ◽  
Cardiovascular Damage ◽  
Scale Expansion

Mesenchymal stem cells (MSCs) are multipotent stem cells with strong immunosuppressive property that renders them an attractive source of cells for cell therapy. MSCs have been studied in multiple clinical trials to treat liver diseases, peripheral nerve damage, graft-versus-host disease, autoimmune diseases, diabetes mellitus, and cardiovascular damage. Millions to hundred millions of MSCs are required per patient depending on the disease, route of administration, frequency of administration, and patient body weight. Multiple large-scale cell expansion strategies have been described in the literature to fetch the cell quantity required for the therapy. In this review, bioprocessing strategies for large-scale expansion of MSCs were systematically reviewed and discussed. The literature search in Medline and Scopus databases identified 26 articles that met the inclusion criteria and were included in this review. These articles described the large-scale expansion of 7 different sources of MSCs using 4 different bioprocessing strategies, i.e., bioreactor, spinner flask, roller bottle, and multilayered flask. The bioreactor, spinner flask, and multilayered flask were more commonly used to upscale the MSCs compared to the roller bottle. Generally, a higher expansion ratio was achieved with the bioreactor and multilayered flask. Importantly, regardless of the bioprocessing strategies, the expanded MSCs were able to maintain its phenotype and potency. In summary, the bioreactor, spinner flask, roller bottle, and multilayered flask can be used for large-scale expansion of MSCs without compromising the cell quality.

Large-scale expansion of mesenchymal stem cells in 3D cultures using xeno-free microcarriers and human platelet lysate

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S110-S111 ◽  
Author(s):  
R.N. Dayment ◽  
C.G. Taylor ◽  
M.Z. Albanna ◽  
K.N. Sarchet ◽  
T.E. Ichim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Human Platelet ◽  
Platelet Lysate ◽  
3D Cultures ◽  
Human Platelet Lysate ◽  
Scale Expansion

Isolation and large-scale expansion of bone marrow-derived mesenchymal stem cells with serum-free media under GMP-compliance

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S111 ◽  
Author(s):  
S.H. Mei ◽  
M. Salkhordeh ◽  
F. Xue ◽  
J. Zhang ◽  
I. Watpool ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Serum Free ◽  
Scale Expansion ◽  
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